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Development and performance evaluation of a culture-independent nanopore amplicon-based sequencing method for accurate typing and antimicrobial resistance profiling in Neisseria gonorrhoeae

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References

  • Antimicrobial Resistance Collaborators. (2022). Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet 399, 629–655.

    Article  Google Scholar 

  • Bissessor, M., Tabrizi, S.N., Fairley, C.K., Danielewski, J., Whitton, B., Bird, S., Garland, S., and Chen, M.Y. (2011). Differing Neisseria gonorrhoeae bacterial loads in the pharynx and rectum in men who have sex with men: implications for gonococcal detection, transmission, and control. J Clin Microbiol 49, 4304–4306.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cabibbe, A.M., Spitaleri, A., Battaglia, S., Colman, R.E., Suresh, A., Up-lekar, S., Rodwell, T.C., and Cirillo, D.M. (2020). Application of targeted next-generation sequencing assay on a portable sequencing platform for culture-free detection of drug-resistant tuberculosis from clinical samples. J Clin Microbiol 58, e00632–20.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Demczuk, W., Sidhu, S., Unemo, M., Whiley, D.M., Allen, V.G., Dillon, J. R., Cole, M., Seah, C., Trembizki, E., Trees, D.L., et al. (2017). Neisseria gonorrhoeae sequence typing for antimicrobial resistance, a novel antimicrobial resistance multilocus typing scheme for tracking global dissemination of N. gonorrhoeae strains. J Clin Microbiol 55, 1454–1468.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu, F., Zhang, Y., Zhang, L., Li, Z., Fang, Q., Gao, R., and Zhang, Z. (2019). Systematic comparative analysis of single-nucleotide variant detection methods from single-cell RNA sequencing data. Genome Biol 20, 242.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Potter, R.F., Ransom, E.M., and Burnham, C.A.D. (2021). The next-generation of Neisseria gonorrhoeae antimicrobial resistance testing. Clin Chem 67, 573–575.

    Article  PubMed  Google Scholar 

  • Priest, D., Ong, J.J., Chow, E.P.F., Tabrizi, S., Phillips, S., Bissessor, M., Fairley, C.K., Bradshaw, C.S., Read, T.R.H., Garland, S., et al. (2017). Neisseria gonorrhoeae DNA bacterial load in men with symptomatic and asymptomatic gonococcal urethritis. Sex Transm Infect 93, 478–481.

    Article  PubMed  Google Scholar 

  • Sereika, M., Kirkegaard, R.H., Karst, S.M., Michaelsen, T.Y., Sørensen, E. A., Wollenberg, R.D., and Albertsen, M. (2022). Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing. Nat Methods 19, 823–826.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yahara, K., Ma, K.C., Mortimer, T.D., Shimuta, K., Nakayama, S., Hirabayashi, A., Suzuki, M., Jinnai, M., Ohya, H., Kuroki, T., et al. (2021). Emergence and evolution of antimicrobial resistance genes and mutations in Neisseria gonorrhoeae. Genome Med 13, 51.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang, C., Xiu, L., Li, Y., Sun, L., Li, Y., Zeng, Y., Wang, F., and Peng, J. (2021). Multiplex PCR and Nanopore sequencing of genes associated with antimicrobial resistance in Neisseria gonorrhoeae directly from clinical samples. Clin Chem 67, 610–620.

    Article  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the CAMS Innovation Fund for Medical Sciences (CIFMS) (2021-I2M-1-037, 2021-I2M-1-038), the National Natural Science Foundation of China (82002193), the National Science and Technology Infrastructure of China (National Pathogen Resource Center-NPRC-32), the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (2019-PT310-029, 2021-PT310-004) and the Fundamental Research Funds for the Central Universities (3332021092). We thank Siyu Zhou for her support with the bioinformatics analysis and script design.

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  1. Contributed equally to this work

Authors and Affiliations

  1. NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China

    Chi Zhang, Lulu Zhang, Liying Sun, Di Wang, Yamei Li, Liqin Wang & Junping Peng

  2. Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences, Beijing, 100730, China

    Chi Zhang, Lulu Zhang, Liying Sun, Di Wang, Yamei Li, Liqin Wang & Junping Peng

  3. Shenzhen Center for Chronic Disease Control, Shenzhen, 518020, China

    Feng Wang & Yaling Zeng

Authors
  1. Chi Zhang
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  2. Lulu Zhang
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  3. Feng Wang
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  4. Yaling Zeng
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  5. Liying Sun
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  6. Di Wang
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  7. Yamei Li
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  8. Liqin Wang
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  9. Junping Peng
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Corresponding author

Correspondence to Junping Peng.

Ethics declarations

Compliance and ethics The authors declare that they have no conflict of interest. This study was performed in accordance with the recommendations of the national ethics regulations and approved by the Institutional Review Board of the Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College (IRB reference number: IPB-2020-12). Clinical samples were collected from the Shenzhen Centre for Chronic Disease Control, and written informed consent was obtained from all participants.

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Development and performance evaluation of a culture-independent nanopore amplicon-based sequencing method for accurate typing and antimicrobial resistance profiling in Neisseria gonorrhoeae

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Zhang, C., Zhang, L., Wang, F. et al. Development and performance evaluation of a culture-independent nanopore amplicon-based sequencing method for accurate typing and antimicrobial resistance profiling in Neisseria gonorrhoeae. Sci. China Life Sci. 67, 421–423 (2024). https://doi.org/10.1007/s11427-022-2382-0

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  • DOI: https://doi.org/10.1007/s11427-022-2382-0

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