Skip to main content
SpringerLink
Log in

Genetically encoded redox biosensor system for H2O2 measurement in four subcellular compartments in acute myeloid leukemia (AML) cells

  • Letter to the Editor
  • Published:
Science China Life Sciences Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Agarwal, R., and Kaye, S.B. (2003). Ovarian cancer: strategies for overcoming resistance to chemotherapy. Nat Rev Cancer 3, 502–516.

    Article  CAS  Google Scholar 

  • Basiouni, S., Fuhrmann, H., and Schumann, J. (2012). High-efficiency transfection of suspension cell lines. Biotechniques 53, 1–4.

    Article  Google Scholar 

  • Belousov, V.V., Fradkov, A.F., Lukyanov, K.A., Staroverov, D.B., Shakhbazov, K.S., Terskikh, A.V., and Lukyanov, S. (2006). Genetically encoded fluorescent indicator for intracellular hydrogen peroxide. Nat Methods 3, 281–286.

    Article  CAS  Google Scholar 

  • Bosc, C., Selak, M.A., and Sarry, J.E. (2017). Resistance is futile: targeting mitochondrial energetics and metabolism to overcome drug resistance in cancer treatment. Cell Metab 26, 705–707.

    Article  CAS  Google Scholar 

  • Bossis, G., Sarry, J.E., Kifagi, C., Ristic, M., Saland, E., Vergez, F., Salem, T., Boutzen, H., Baik, H., Brockly, F., et al. (2014). The ROS/SUMO axis contributes to the response of acute myeloid leukemia cells to chemotherapeutic drugs. Cell Rep 7, 1815–1823.

    Article  CAS  Google Scholar 

  • Irwin, M.E., Rivera-Del Valle, N., and Chandra, J. (2013). Redox control of leukemia: from molecular mechanisms to therapeutic opportunities. Antioxid Redox Signal 18, 1349–1383.

    Article  CAS  Google Scholar 

  • Melo, E.P., Lopes, C., Gollwitzer, P., Lortz, S., Lenzen, S., Mehmeti, I., Kaminski, C.F., Ron, D., and Avezov, E. (2017). TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2. BMC Biol 15, 24.

    Article  Google Scholar 

  • Passos, J.F., Miwa, S., and von Zglinicki, T. (2013). Measuring reactive oxygen species in senescent cells. In: Galluzzi, L., Vitale, I., Kepp, O., and Kroemer, G., eds. Cell Senescence. Methods in Molecular Biology (Methods and Protocols). Totowa: Humana Press. 253–263.

    Chapter  Google Scholar 

  • Provost, C., Choufani, F., Avedanian, L., Bkaily, G., Gobeil, F., and Jacques, D. (2010). Nitric oxide and reactive oxygen species in the nucleus revisited. Can J Physiol Pharmacol 88, 296–304.

    Article  CAS  Google Scholar 

  • Zou, Y., Wang, A., Shi, M., Chen, X., Liu, R., Li, T., Zhang, C., Zhang, Z., Zhu, L., Ju, Z., et al. (2018). Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors. Nat Protoc 13, 2362–2386.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Key Research and Development Program of China (2017YFA0205500 and 2018YFA0902702), the National Natural Science Foundation of China (21890743), and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB29050100). We thank Junying Jia and Shu Meng for technical assistance in FACS analysis (FACS center, Institute of Biophysics (IBP), Chinese Academy of Sciences (CAS)) and Shuoguo Li and Yan Teng (Center for Biological Imaging (CBI), IBP-CAS) for SIM imaging. We gratefully acknowledge Prof. Yu Zhang for Nanoparticles support (Southeast University, Nanjing, China).

Author information

Authors and Affiliations

  1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Mengmeng Cui, Ghulam Abbas, Dianbing Wang, Qian Liu, Rui Gong, Min Li & Xian-En Zhang

  2. Faculty of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China

    Xian-En Zhang

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Ghulam Abbas, Rui Gong & Xian-En Zhang

Authors
  1. Mengmeng Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ghulam Abbas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dianbing Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qian Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rui Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Min Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xian-En Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xian-En Zhang.

Additional information

Compliance and ethics

The author(s) declare that they have no conflict of interest.

Electronic Supplementary Material

11427_2021_2049_MOESM1_ESM.docx

Genetically encoded redox biosensor system for H2O2 measurement in four subcellular compartments in acute myeloid leukemia (AML) cells

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Cui, M., Abbas, G., Wang, D. et al. Genetically encoded redox biosensor system for H2O2 measurement in four subcellular compartments in acute myeloid leukemia (AML) cells. Sci. China Life Sci. 65, 1259–1262 (2022). https://doi.org/10.1007/s11427-021-2049-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11427-021-2049-6

Search

Navigation