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Cloning and identification of a novel RNF6 transcriptional splice variant Spg2 in human development

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Abstract

RING finger proteins are zinc finger proteins containing the RING motifs. They act mainly as E3 ubiquitin ligases, bind the ubiquitin E2 conjugating enzyme and promote degradation of targeted proteins, Many novel genes have been isolated and differentially expressed in human adult and embryo testis by a testis cDNA-array differential display technique. A novel RING finger cDNA is highly expressed in adult testis and at low level in fetal testis. It was named Spg2. It contains a 2055 nucleotide ORF, encodes a 685-amino-acid RNF6 protein, and has a RING finger in its C terminal. NCBI Blast shows that the gene is located on chromosome 13 and contains five exons. A multiple tissue expression profile also indicates that it is highly expressed in human testis, so we speculate that it may be associated with human spermatogenesis by virtue of the action of its RING domain.

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Authors and Affiliations

  1. Lab of Reproductive Medicine, Department of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing, 210029, China

    Li Zhu, Jie Chen, Yun Wang, SaiQun Wang, MuZhi Zhao, JianMin Li & JianHua Ma

  2. Department of Endocrinology, Nanjing First Hospital, The Third Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China

    JianHua Ma

  3. Dapartment of Obstetrics and Gynecology, Nanjing Maternity and Child Health Care Hospital, Nanjing, 210029, China

    GuoQin Tong

Authors
  1. Li Zhu
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  2. GuoQin Tong
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  3. Jie Chen
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  4. Yun Wang
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  5. SaiQun Wang
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  6. MuZhi Zhao
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  7. JianMin Li
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  8. JianHua Ma
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Corresponding authors

Correspondence to JianMin Li or JianHua Ma.

Additional information

Contributed equally to this work

Supported by the National Basic Research Program (973 Program) of China (Grant No. G1999055901) and National Natural Science Foundation of China (Grant No. 30000088)

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Zhu, L., Tong, G., Chen, J. et al. Cloning and identification of a novel RNF6 transcriptional splice variant Spg2 in human development. SCI CHINA SER C 51, 302–307 (2008). https://doi.org/10.1007/s11427-008-0047-3

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  • DOI: https://doi.org/10.1007/s11427-008-0047-3

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