Abstract
Mycoplasma are wall-less bacteria with small genomes, which are thought to have resulted from massive genome reductive processes, during which the ori-ter balance may be disrupted. For technical difficulties, ori and ter have been located only in a few Mycoplasma strains. Using the Z curve method, we were able to locate turning points on the Mycoplasma genomes, with the minimum and maximum points co-locating with ori or ter in the reference genomes. Assuming Z curve correctly located ori and ter, we calculated the distances from ori to ter in both directions on the circular genome and calculated the ori-ter balance status. The Mycoplasma genomes were not balanced, possibly as a result of close association of Mycoplasma with hosts, where there would be no other microbes for Mycoplasma to compete with for nutrients, so fastest possible growth related to balanced genomes might not be needed by Mycoplasma, leading to a relaxing ori-ter balance.
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Supported by the National Natural Science Foundation of China (Grant No. 30370774), the 985 Project of Peking University Health Science Center, and the Discovery Grant from Natural Sciences and Engineering Research Council of Canada (Grant No. 216912-00)
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Zheng, X., Liu, S. The relaxing ori-ter balance of Mycoplasma genomes. Sci. China Ser. C-Life Sci. 51, 182–189 (2008). https://doi.org/10.1007/s11427-008-0017-9
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DOI: https://doi.org/10.1007/s11427-008-0017-9