Abstract
After injecting VP16, MEL cells and MEL-TF19 cells into the body of mice, with those injected with the same dose of saline as the control group, we observed the mice for their blood pictures, histological changes of the liver and spleen, and the hemorhelogical indexes within 4 weeks. The results indicated that after injecting MEL cells, the mice entered into a pathological status similar to erythroleukemia, which had the following exhibitions: the tissue structures of the liver and spleen were damaged, a mass of proerythroblasts, basophil erythroblasts and polychromatophilic erythroblasts could be observed on the smears of the bone marrow and spleen, and the deformability and orientation ability of erythrocytes were both depressed. The pathogenicity of MEL-TF19 cells carrying TFAR19 gene was obviously lower than that of MEL cells, and the MEL-TF19 cells even lost their faintish pathogenicity under the apoptosis-inducing effect of the chemotherapeutic reagent. The outcome from the animal experiments suggests that the TFAR19 gene suppresses the pathogenicity of MEL cells to the mice, and the effect may be better exerted with the synergy of the chemotherapeutic reagent.
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References
Evan G, Little Wood T. A matter of life and cell death. Science, 1998, 281: 1317–1321
Ashkenazi A, Dixit V M. Death receptors: Signal and modulation. Science, 1998, 281: 1305–1308
Yao W J, Liang Y, Chen K, et al. Changes of biophysical behavior of k562 cells for p16 gene transfer. Clinical Hemorheology and Micro-circulation, 2002, 27: 177–183
Chen K, Li D, Yao W J, et al. Influence of TRAIL gene on biophysical cell line Jurkat. Cell Res, 2004, 14(2): 161–168
Sun D G, Yao W J, Wang J H, et al. Tumorigenesis of murine erythroleukemia cell line transfected with exogenous p53 gene. Clinical Hemorheology and Microcirculation, 2004, 30(2): 117–126
Yao W J, Chen K, Wang X J, et al. Influence of TRAIL gene on biomechanics properties of the human leukemic cell line Jurkat. J Biomech, 2002, 35: 1659–1663
Liu H, Wang Y, Zhang Y, et al. Study of cDNA cloning, expression and function of a novel apoptosis related gene TFAR19. High Technol Lett, 1998, 8(5): 6–10
Liu H, Wang Y, Zhang Y, et al. TFAR19, a novel apoptosis related gene cloned from human Leukemia cell line TF21, could enhance apoptosis of some tumor cells induced of growth factor withdrawal. Biochem Biophys Res Commun, 1999, 254(1): 203–210
Gu L, Jiang Y H, Wang Y, et al. TFAR19 gene changes biophysical behavior of murine erythroleukemia cells. Cell Biochem Biophys, 2005, 43: 355–363
Gu L, Yao W J, Xie L D, et al. Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL. Sci China Ser C-Life Sci (in Chinese), 2002, 32(5): 447–453
Gu L, Xie L D, Yao W J, et al. FTIR spectroscopy studies on the apoptosis-promoting effect of TFAR19 on the erythroleukem in cell line MEL. J Biomed Eng (in Chinese), 2004, 21(3): 449–452
Lin K C, Wu B J. Medical Biophysics (in Chinese). Beijing: Beijing Medical University & Peking Union Medical College Union Press, 1996. 130
Wu Z Z, Zhang G, Long M, et al. Comparison of the viscoelastic properties of normal hepatocytes and hepatocellular carcinoma cells under cytoskeletal perturbation,. Biorheology, 2000, 37: 279–90
Wen Z Y, Tao Z L, Ma W Y, et al. The influence of surface charge of RBC membrane upon its orientation in a shear flow field of low viscosity. Clinical Hemorheology, 1994, 14: 279–283
Yao W J, Wen Z Y, Yan Z Y, et al. Low viscosity Ektacytometry and its validation tested by flow chamber. J Biomech, 2001, 34: 1501–1509
Wen Z Y, Yao W J, Xie L D, et al. Influence of neuraminidase on the characteristics of microrheology of red blood cells. Clinical Hemorheology and Microcirculation, 2000, 23: 51–57
Wen Z Y, Yan Z Y, Gao T, et al. A study of effects of WGA and ConA on RBC membrane receptors using a new ektacytometric method. Clinical Hemorheology and Microcirculation, 1997, 17: 467–478
Friend C. Cell-free transmission in adult Swiss mice of a disease having the character of leukemia. J Exp Med, 1957, 105: 307–318
Rauscher F J. A virus-induced disease of mice characterized by erythrocytopoiesis and lymphoid leukemia. J Natn Cancer Inst, 1962, 29: 515–543
Itoh K, Ono K, Swada H, et al. Establishment and characterization of a transplantable erythroblastic leukemia in C3H. Leukemia Res, 1988, 12: 471–478
Ding S L, Zhu J X, Zhao J M, et al. Establishment and biological characterization of a transplantable BALB/c mouse erythroblastic leukemia model. Chin J Hematol (in Chinese), 1998, 19(12): 638–641
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Supported by the National Natural Science Foundation of China (Grant Nos. 30270355 & 10572007)
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Gu, L., Tang, Z., He, D. et al. Effects of TFAR19 gene on the in vivo biorheological properties and pathogenicity of mouse erythroleukemia cell line MEL. SCI CHINA SER C 50, 111–119 (2007). https://doi.org/10.1007/s11427-007-0007-3
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DOI: https://doi.org/10.1007/s11427-007-0007-3