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Recent advances in the preparation of Fmoc-SPPS-based peptide thioester and its surrogates for NCL-type reactions

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Abstract

Solid phase peptide synthesis (SPPS) based on Fmoc chemistry has become a commonly used technique in peptide chemistry, as it can be easily conducted using automated machine, and not requiring highly toxic HF in comparison to Boc-SPPS. With the fast development in the emerging field of protein chemical synthesis, many efforts have been endeavored aiming to find more efficient methods for preparing peptide fragments required in ligation reactions. This review briefly summarizes recent advances in the engineering and modification of Fmoc-SPPS-derived peptides, which can be used as the N-terminal fragments in a native chemical ligation (NCL) or NCL-type ligation reactions.

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Acknowledgments

This work was supported by the Peking University Health Science Center (BMU20130354), State Key Laboratory of Natural and Biomimetic Drugs, the National Recruitment Program of Global Youth Experts (1000 Plan), and the National Natural Science Foundation of China (21502005).

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  1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China

    Hongxing Li & Suwei Dong

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  1. Hongxing Li
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Correspondence to Suwei Dong.

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Li, H., Dong, S. Recent advances in the preparation of Fmoc-SPPS-based peptide thioester and its surrogates for NCL-type reactions. Sci. China Chem. 60, 201–213 (2017). https://doi.org/10.1007/s11426-016-0381-1

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