Skip to main content
SpringerLink
Log in

Mixed alkaline phosphatase/sphingomyelin monolayer at the air-buffer interface: phase behavior and morphology

  • Articles
  • Published:
Science China Chemistry Aims and scope Submit manuscript

Abstract

The glycosylphosphatidyl inositol (GPI)-anchored proteins are localized on the outer of the plasma membrane and clustered in membrane microdomain known as lipid rafts. Among them, mammalian alkaline phosphatase (AP) is an enzyme widely distributed. So, it has important biological significance to study the combination of AP with lipid monolayer. In our work, the interaction between AP and sphingomyelin has been studied at the air-buffer interface as a biomimetic membrane system by the Langmuir film technique and atomic force microscopy. The surface pressure-area isotherm for the mixed alkaline phosphatase/sphingomyelin monolayer shown the presence of a transition from a liquid-expanded phase to the liquid-expanded/liquid-condensed coexist phase. And the surface compressional modulus suggested the mixed alkaline phosphatase/sphingomyelin monolayer has larger compressibility compared with the pure sphingomyelin monolayer. Besides, according to the micrographs, we inferred when combined with lipid monolayer at the air-buffer interface, the AP molecules formed polymer not multilayer or micelle. And, according to the limiting molecules area of AP, we inferred that 12 AP molecules formed a hexagon polymer unit.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Referemces

  1. Alexandra M, Christine K, Megan SV, Judith VH, Martin S. Selective and programmed cleavage of GPI-anchored proteins from the surface membrane by phospholipase C. Biochimica et Biophysica Acta, 2012, 1818: 117–124

    Article  Google Scholar 

  2. Ronzon F, Desbat B, Chauvet JP, Roux B. Behavior of a GPI-anchored protein in phospholipid monolayers at the air-water interface. Biochimica et Biophysica Acta, 2002, 1560: 1–13

    Article  CAS  Google Scholar 

  3. Calvez P, Bussieres S, Demers E, Salesse C. Parameters modulating the maximum insertion pressure of proteins and peptides in lipid monolayers. Biochimie, 2009, 91: 718–733

    Article  CAS  Google Scholar 

  4. Yasuko T, Yuko N, Hironobu H, Yoshiaki N. Studies directed toward the synthesis of protein-bound GPI anchor. Tetrahedron, 2003, 59: 4059–4067

    Article  Google Scholar 

  5. Morihisa F, Taroh K. GPI-anchor remodeling: potential functions of GPI-anchors in intracellular trafficking and membrane dynamics. Biochimica et Biophysica Acta, 2012, 1821:1050–1058

    Article  Google Scholar 

  6. Christoph M, Brian S, Walter H, John A. Danger field rafts anchors and viruses: a role for glycosylphosphatidyl inositol anchored proteins in the modification of enveloped viruses and viral vectors. Virology, 2008, 382: 125–131

    Article  Google Scholar 

  7. Calvez P, Bussieres S, Demers E, Salesse C. Parameters modulating the maximum insertion pressure of proteins and peptides in lipid monolayers. Biochimie, 2009, 91: 718–733

    Article  CAS  Google Scholar 

  8. Fernando LC, Katia RPD, Prislaine PM, João MP, Pietro C. Construction of an alkaline phosphatase-liposome system: a tool for biomineralization study. Int J Biochem Cell B, 2002, 34: 1091–1101

    Article  Google Scholar 

  9. Bolean M, Simão AMS, Favarin BZ, Millán JL, Ciancaglini P. Thermodynamic properties and characterization of proteoliposomes rich in microdomains carrying alkaline phosphatase. Biophys Chem, 2011, 158: 111–118

    Article  CAS  Google Scholar 

  10. Bolean M, Simão AMS, Favarin BZ, Millán JL, Ciancaglini P. The effect of cholesterol on the reconstitution of alkaline phosphatase into liposomes. Biophys Chem, 2010, 152: 74–79

    Article  CAS  Google Scholar 

  11. Daniela FI, Joao MP, Pietro C. Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineral ization studies. Biochimica et Biophysica Acta, 2002, 1567: 183–192

    Article  Google Scholar 

  12. Caseli L, Zaniquelli MED, Furriel RPM, Leone FA. Enzymatic activity of alkaline phosphatase adsorbed on dimyristoylphosphatidic acid Langmuir-Blodgett films. Colloid Surface B, 2002, 25: 119–128

    Article  CAS  Google Scholar 

  13. Caseli L, Maria DZ, Furriel RPM, Leone FA, Zaniquelli MED, Orbulescu J, Leblanc RM. Rat osseous plate alkaline phosphatase as Langmuir monolayer: an infrared study at the air-water interface. J Colloid Interf Sci, 2008, 320: 476–482

    Article  CAS  Google Scholar 

  14. Agnes PG, Stephanie G, Loic JB. Enzyme association with lipidic Langmuir-Blodgett films: interests and applications in nanobioscience. Adv Colloid Interfac Sci, 2005, 116: 205–225

    Article  Google Scholar 

  15. Luciano C, Douglas CM, Rosa PMF, Francisco AL, Maria EDZ. Influence of the glycosylphosphatidylinositol anchor in the morphology and roughness of Langmuir-Blodgett films of phospholipids containing alkaline phosphatases. Thin Solid Films, 2007, 515: 4801–4807

    Article  Google Scholar 

  16. Frederic R, Bernard D, Jean-Paul C, Bernard R. Penetration of a GPI-anchored protein into phospholipid monolayers spread at the air/water interface. Colloid Surface B, 2002, 23: 365–373

    Article  Google Scholar 

  17. Achraf K, Francoise B. GPI-alkaline phosphatase insertion into phosphatidylcholine monolayers: phase behavior and morphology changes. Biochem Bioph Res Co, 2005, 333: 1315–1321

    Article  Google Scholar 

  18. Luciano C, Douglas CM, Rosa PMF, Francisco AL, Maria EDZ. Incorporation conditions guiding the aggregation of a glycosylphosphatidylinositol (GPI)-anchored protein in Langmuir monolayers. Colloid Surface B, 2005, 46: 248–254

    Article  Google Scholar 

  19. Zheludeva S, Novikova N, Stepina N, Yurieva E, Konovalov O. Molecular organization in protein-lipid film on the water surface studied by x-ray standing wave measurements under total external reflection. Spectrochim Acta B, 2008, 63: 1399–1403

    Article  Google Scholar 

  20. Luciano C, Frank NC, Thatyane MN, Maria EDZ, Valtencir Z, Osvaldo Jr NO. Using phospholipid Langmuir and Langmuir-Blodgett films as matrix for urease immobilization. J Colloid Interf Sci, 2008, 319: 100–108

    Article  Google Scholar 

  21. Peng JB, Barnes GT, Gentle IR. The structures of Langmuir-Blodgett films of fatty acids and their salts. Adv Colloid Interface Sci, 2001, 91: 163–219

    Article  CAS  Google Scholar 

  22. Gerhard S, Jiayun Zh. Chain length dependence of lipid partitioning between the air/water interface and its subphase. Chem Phys Lipids, 2001, 110: 35–45

    Article  Google Scholar 

  23. Hao CC, Sun RG, Zhang J, Chang YG, Niu CL. Behavior of sulfatide/cholesterol mixed monolayers at the air/water interface. Colloid Surface B, 2009, 69: 201–206

    Article  CAS  Google Scholar 

  24. Zakanda FN, Laurent P, Paquot M, Lelo GM, Deleu M. Alkylbetainate chlorides: synthesis and behavior of monolayers at the-air-water interface. Thin Solid Films, 2011, 520: 344–350

    Article  CAS  Google Scholar 

  25. Amiya Kumar P, Vasilev K, Orgeig S, Clive AP. Thermodynamic and structural studies of mixed monolayers: mutual mixing of DPPC and DPPG with DTAP at the air-water interface. Mater Sci Eng C, 2010, 30: 542–548

    Article  Google Scholar 

  26. Karen S, Juha-Pekka M, Paavo KJK. Interfacial behavior of cholesterol, ergosterol, and lanosterol in mixtures with DPPC and DMPC. Biophys J, 2008, 95: 2340–2355

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Biophysics and Biomedicine, College of Physics and Information Technology, Shaanxi Normal University, Xi’an, 710062, China

    Juan Wang, RunGuang Sun & ChangChun Hao

Authors
  1. Juan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. RunGuang Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. ChangChun Hao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to RunGuang Sun or ChangChun Hao.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, J., Sun, R. & Hao, C. Mixed alkaline phosphatase/sphingomyelin monolayer at the air-buffer interface: phase behavior and morphology. Sci. China Chem. 57, 1538–1543 (2014). https://doi.org/10.1007/s11426-014-5124-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11426-014-5124-9

Keywords

Search

Navigation