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Assembly of layer-by-layer films of superoxide dismutase and gold nanorods: A third generation biosensor for superoxide anion

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Abstract

Based on the layer-by-layer self-assembly of positively charged cetyltrimethylammonium bromide (CTAB) wrapped gold nanorods (AuNRs) and negatively charged superoxide dismutase (SOD) from their aqueous solutions on cysteine modified gold electrode (Cys/Au), a third generation electrochemical biosensor ((SOD/AuNRs)2/Cys/Au) for superoxide anion (O2 ·−) was developed. The two layers assembly of SOD/AuNRs can significantly enhance the direct electron transfer between SOD and the electrode. The functional enzymatic activities of the SOD offer an electrochemical approach to the determination of O2 ·−. In the reductive regions, the proposed sensor exhibits excellent analytical performances, such as wide linear range (200 nM to 0.2 mM O2 ·−), low detection limit (100 nM O2 ·−), high sensitivity (22.11 nA cm−2 μM−1), short response time (less than 5 s), good stability and reproducibility, while no obvious interferences are caused by commonly met interfering species including hydrogen peroxide (H2O2), uric acid (UA) and ascorbic acid (AA).

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Authors and Affiliations

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China

    MengDong Wang, YiTao Han, XingXing Liu, Zhou Nie, ManLi Guo & ShouZhuo Yao

  2. College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China

    ChunYan Deng

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  1. MengDong Wang
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  2. YiTao Han
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  3. XingXing Liu
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  4. Zhou Nie
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  5. ChunYan Deng
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  6. ManLi Guo
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  7. ShouZhuo Yao
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Correspondence to Zhou Nie.

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Wang, M., Han, Y., Liu, X. et al. Assembly of layer-by-layer films of superoxide dismutase and gold nanorods: A third generation biosensor for superoxide anion. Sci. China Chem. 54, 1284–1291 (2011). https://doi.org/10.1007/s11426-011-4345-4

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  • DOI: https://doi.org/10.1007/s11426-011-4345-4

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