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Cytotoxicity of liver targeted drug-loaded alginate nanoparticles

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Abstract

In this study, novel liver targeted doxorubicin (DOX) loaded alginate (ALG) nanoparticles were prepared by CaCl2 crosslinking method. Glycyrrhetinic acid (GA, a liver targeted molecule) modified alginate (GA-ALG) was synthesized in a heterogeneous system, and the structure of GA-ALG and the substitution degree of GA were analyzed by 1H NMR, FT-IR and elemental analysis. The drug release profile under the simulated physiological condition and cytotoxicity experiments of drug-loaded GA-ALG nanoparticles were carried out in vitro. Transmission electron micrographs (TEM) and dynamic light scattering (DLS) analysis showed that drug-loaded GA-ALG nanoparticles have spherical shape structure with the mean hydrodynamic diameter around 214 ± 11 nm. The drug release was shown to last 20 days, and the MTT assay suggested that drug-loaded GA-ALG nanoparticles had a distinct killing effect on 7703 hepatocellular carcinoma cells.

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Correspondence to Zhi Yuan.

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Supported by the National Natural Science Foundation of China (Grant No. 20634030), Key Natural Science Fund of Tianjin (Grant No. 07JCZDJC00700) and State Key Lab of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Science Funds

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Zhang, C., Wang, W., Wang, C. et al. Cytotoxicity of liver targeted drug-loaded alginate nanoparticles. Sci. China Ser. B-Chem. 52, 1382–1387 (2009). https://doi.org/10.1007/s11426-009-0178-9

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  • DOI: https://doi.org/10.1007/s11426-009-0178-9

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