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A new approach in tandem mass spectrometric simultaneous determination of perchlorate, thiocyanate, and iodide using their ternary complexes with tris(1,10-phenanthroline)iron(II) chelate

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Abstract

A rapid and sensitive method was developed for simultaneous determination of perchlorate (ClO4 −), thiocyanate (SCN−), and iodide (I−). The ions were reacted with tris(1,10-phenanthroline)iron(II) chelating cation, [Fe(phen)3]2+, to produce their ternary complexes, [Fe(phen)2ClO4]+, [Fe(phen)2SCN]+, and [Fe(phen)2I]+ that were extracted together with nitrobenzene. Five microliters of the extract was injected directly into an electrospray ionization tandem mass spectrometer. Quantification of ClO4 −, SCN−, and I− was performed by selected reaction monitoring of the product ion [Fe(phen)O2]+ at m/z 268 that derived from the precursor ion [Fe(phen)2ClO4]+ at m/z 515, the product ion [Fe(phen)SCN]+ at m/z 294 that derived from precursor ion [Fe(phen)2SCN]+ at m/z 474, and the product ion [Fe(phen)I]+ at m/z 363 that derived from precursor ion [Fe(phen)2I]+ at m/z 543, respectively. Perchlorate, SCN−, and I− were measured in the range of 0.1–10 μM with the limits of detection at 0.03 μM by using a 50-μl sample aliquot containing the three ions within 10 min. The SCN− level in gastric fluid of a victim of NaCN poisoning was 137 ± 23 μM, which is slightly higher than the results from control subjects (n = 7), 72 ± 39 μM.

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Authors and Affiliations

  1. Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan

    Kayoko Minakata, Hideki Nozawa, Itaru Yamagishi, Kunio Gonmori, Koutaro Hasegawa, Amin Wurita, Kanako Watanabe & Osamu Suzuki

  2. Research Equipment Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan

    Masako Suzuki

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  1. Kayoko Minakata
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  2. Hideki Nozawa
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  3. Itaru Yamagishi
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  4. Kunio Gonmori
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  5. Koutaro Hasegawa
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  6. Masako Suzuki
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  7. Amin Wurita
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  8. Kanako Watanabe
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  9. Osamu Suzuki
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Correspondence to Kayoko Minakata.

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Minakata, K., Nozawa, H., Yamagishi, I. et al. A new approach in tandem mass spectrometric simultaneous determination of perchlorate, thiocyanate, and iodide using their ternary complexes with tris(1,10-phenanthroline)iron(II) chelate. Forensic Toxicol 30, 156–161 (2012). https://doi.org/10.1007/s11419-012-0140-y

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  • DOI: https://doi.org/10.1007/s11419-012-0140-y

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