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Soshiho-tang protects LPS-induced acute liver injury by attenuating inflammatory response

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Abstract

Soshiho-tang (SSHT) has traditionally been used to treat gastrointestinal disorders. In this experiment, we investigated the protective effect of SSHT on inflammatory liver injury in lipopolysaccharide (LPS)-sensitized mice. Male C57BL/6J mice aged 6 weeks were randomly placed in 6 groups (n = 5): normal mice (CTR), LPS-sensitized mice (LPS), LPS-sensitized mice treated with dexamethasone (DEX) and LPS-sensitized mice treated with 0.05, 0.55, and 5.55 g/kg of SSHT (SSHT 0.05, SSHT 0.55, and SSHT 5.55). Various doses of SSHT was given once a day for 7 days. After 2 h of LPS injection, the liver tissue was collected. SSHT pretreatment recovered hemorrhage of liver tissues in LPS-induced acute liver injury. The expressions of MAP Kinase, NF-κB, IκBα, p-IκBα, COX-2, and iNOS protein levels were markedly decreased by SSHT-treated liver tissues. Additionally, SSHT pretreatment significantly regulated the expressions of MCP-1, TNF-α, and IL-6 cytokines. These results suggest the potential of SSHT on the protection of acute liver injury.

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Acknowledgements

This work was supported by a grant of the National Development Institute of Korean Medicine (NIKOM) funded by the Korean Ministry of Health and Welfare (MOHW), Republic of Korea, and a National Research Foundation of Korea Grant funded by the Korean government (NRF-2019R1I1A2A01063598).

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Authors and Affiliations

  1. Department of Convergence Korean Medical Science, Graduate School, College of Korean Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea

    Seong Chul Jin, Mi Hye Kim, Si Yeon Jo, La Yoon Choi, Haesu Lee & Woong Mo Yang

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  1. Seong Chul Jin
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  2. Mi Hye Kim
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Jin, S.C., Kim, M.H., Jo, S.Y. et al. Soshiho-tang protects LPS-induced acute liver injury by attenuating inflammatory response. J Nat Med 74, 788–795 (2020). https://doi.org/10.1007/s11418-020-01421-w

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