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Enzyme linked immunosorbent assay for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei, a Thai herb for menopause remedy

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Abstract

Miroestrol and deoxymiroestrol are the most potent phytoestrogens of Pueraria candollei var. mirifica, having been proved as an effective herb for menopausal symptoms in folk medicines and clinical trials. To ensure efficacy and safety of P. candollei var. mirifica involved in nutraceutical products being available worldwide, the content of potent phytoestrogens as active ingredients should be specified. Therefore, in this study, we produced a monoclonal antibody for total analysis of potent estrogenic miroestrol and deoxymiroestrol, for which an analytical method was developed using a procedure for an indirect competitive enzyme-linked immunosorbent assay. The antibody exhibited equal reactivity against miroestrol and deoxymiroestrol. The sensitivity of determination was in the range of 31.3–500 ng/ml with high precision. The analytical parameters, such as accuracy (99.6–106% recovery) and high correlation with a HPLC–UV method, indicated the reliability of analysis. This method is of high performance, and it is cheap to control optimal miroestrol and deoxymiroestrol doses of P. candollei var. mirifica nutraceutical products.

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Acknowledgements

This work was supported by the Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand. This work has received a scholarship under the Post-Doctoral Training Program from Research Affairs and Graduate School, Khon Kaen University, Thailand (Grant No. 58439). The authors thank Dr. Chaiyo Chaichantipyuth, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, for providing miroestrol and deoxymiroestrol.

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Authors and Affiliations

  1. Drug and Cosmetics Excellence Center, Walailak University, Thaiburi, Thasala, Nakhon Si Thammarat, 80160, Thailand

    Gorawit Yusakul

  2. School of Pharmacy, Walailak University, Thaiburi, Thasala, Nakhon Si Thammarat, 80160, Thailand

    Gorawit Yusakul

  3. Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Rajthevi, Bangkok, 10400, Thailand

    Tharita Kitisripanya

  4. Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand

    Thaweesak Juengwatanatrakul

  5. Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812–8582, Japan

    Seiichi Sakamoto & Hiroyuki Tanaka

  6. Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Waraporn Putalun

  7. Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, 40002, Thailand

    Waraporn Putalun

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  1. Gorawit Yusakul
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  2. Tharita Kitisripanya
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  3. Thaweesak Juengwatanatrakul
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Correspondence to Waraporn Putalun.

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Yusakul, G., Kitisripanya, T., Juengwatanatrakul, T. et al. Enzyme linked immunosorbent assay for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei, a Thai herb for menopause remedy. J Nat Med 72, 641–650 (2018). https://doi.org/10.1007/s11418-018-1194-x

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  • DOI: https://doi.org/10.1007/s11418-018-1194-x

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