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Antiviral effects of Yinhuapinggan granule against influenza virus infection in the ICR mice model

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Abstract

Yinhuapinggan granule (YHPG), a Chinese medicine granule based on Ma-Huang-Tang (Ephedra Decoction) and the clinical experience of Professor Wan Haitong, is used in traditional Chinese medicine (TCM) for the treatment of colds, influenza, fever, inflammation and cough. This study investigated the antiviral effects of YHPG on the production of inflammatory cytokines in influenza virus (IFV)-infected mice and evaluated the effect of YHPG on the expression of NF-κB p65 and the level of key signaling molecules in the TLR4 signaling pathway. ICR mice were orally administrated YHPG at doses of 7.5, 15 and 30 g kg−1 day−1 for 2 or 6 days after IFV infection. On days 3 and 7 after infection, YHPG (15 g/kg and 30 g/kg) significantly increased levels of interleukin (IL)-2 and interferon gamma and decreased levels of IL-4, IL-5 and tumor necrosis factor (TNF) in serum compared with the IFV control group. Furthermore, the expression of TLR4, MyD88, TRAF6 and NF-κB p65 at the mRNA and protein level was significantly lower in the YHPG (15 and 30 g/kg) treatment groups than in the IFV control group. These results suggest that YHPG has antiviral effects in IFV-infected mice, which is associated with the inhibition of the TLR4−MyD88−TRAF6 signaling pathway and the expression of NF-κB p65.

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Acknowledgment

This work was supported by a grant from National Science Foundation of China (No. 81573868), Zhejiang Provincial Natural Science Foundation (Nos. LZ14H270001 and LR13H270001), Zhejiang Province Science and Technology Project (No. 2015C37077). We thank Professor Yi-yu Lu for technical help in the animal experiments and helpful discussion regarding this work.

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Authors and Affiliations

  1. Zhejiang Chinese Medical University, Hangzhou, 310053, China

    Xue-qian Peng, Hui-fen Zhou, Yu-yan Zhang & Jie-hong Yang

  2. Institute of Cardio-Cerebrovascular Diseases, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, China

    Hai-tong Wan

  3. College of Pharmaceutical Science, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, China

    Yu He

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  1. Xue-qian Peng
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  2. Hui-fen Zhou
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  4. Jie-hong Yang
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  5. Hai-tong Wan
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Correspondence to Hai-tong Wan or Yu He.

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Peng, Xq., Zhou, Hf., Zhang, Yy. et al. Antiviral effects of Yinhuapinggan granule against influenza virus infection in the ICR mice model. J Nat Med 70, 75–88 (2016). https://doi.org/10.1007/s11418-015-0939-z

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  • DOI: https://doi.org/10.1007/s11418-015-0939-z

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