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Inoculating exogenous bacterium Brevibacillus laterosporus ZR-11 at maturity stage accelerates composting maturation by regulating physicochemical parameters and indigenous bacterial community succession

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Abstract

Brevibacillus laterosporus ZR-11, a bio-control strain, was innovatively inoculated at maturity stage of composting to clarify its effect on physicochemical parameters and indigenous bacterial community structure in compost pile. Results revealed that ZR-11 inoculum rapidly increased pile temperature to 52 ºC and raised germination index (GI) value to beyond 85% on day 3, thereby achieving higher pile temperature and GI in the inoculated group than the non-inoculated group almost along maturity stage, and also decreased C/N ratio of the inoculated group to below 20 by composting end (day 8). Also, ZR-11 succeeded in colonizing compost pile along maturity stage. These suggested that ZR-11 as inoculum at maturity stage could accelerate compost maturation and have a potential to participate in bio-fertilizer production. High-throughput sequencing indicated that bacterial community structure experienced substantial succession in the inoculated and non-inoculated groups, and Firmicutes, Proteobacteria, and Actinobacteria were the dominant phyla in the two groups during maturity stage, with their abundances higher in the inoculated group. Saccharomonospora and Ammoniibacillus abundance increased on day 3 while Actinomadura abundance increased on day 6 in the inoculated group. As verified statistically, pile temperature and pH were key factors closely linked to dominant genera abundance, where Saccharomonospora and Ammoniibacillus abundance were positively correlated to pile temperature, while Actinomadura abundance was positively correlated to pile pH. Thus, it was inferred that ZR-11 inoculum could improve parameters such as temperature and pH to modify dominant genera abundance, thus regulating indigenous bacterial community succession, which might in turn promote compost maturation.

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Funding

This research was funded by the National Key Research and Development Program of China (Grant number: 2018YFD0500202).

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Authors and Affiliations

  1. College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China

    Li Ren, Jieming Li, Zhonghui Guo & Ji Li

  2. Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, 100193, China

    Li Ren, Jieming Li, Zhonghui Guo & Ji Li

  3. Bluestar Lehigh Engineering Institute Co., Ltd, Lianyungang, 222000, China

    Huifen Li

  4. College of Land Science and Technology, China Agricultural University, Beijing, 100193, China

    Yizhong Lv

Authors
  1. Li Ren
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  2. Jieming Li
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  4. Zhonghui Guo
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  5. Ji Li
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Contributions

Li Ren conducted the formal analysis and investigation and contributed to the writing of the original draft. Jieming Li contributed to the conceptualization, formal analysis, investigation, resource allocation, writing the original draft, reviewing and editing, visualization, supervision, and securing funding. Huifen Li contributed to the formal analysis. Zhonghui Guo, Ji Li, and Yizhong Lv were involved in the formal analysis.

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Correspondence to Jieming Li.

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Ren, L., Li, J., Li, H. et al. Inoculating exogenous bacterium Brevibacillus laterosporus ZR-11 at maturity stage accelerates composting maturation by regulating physicochemical parameters and indigenous bacterial community succession. Environ Sci Pollut Res 30, 110888–110900 (2023). https://doi.org/10.1007/s11356-023-30091-w

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