Abstract
Biopesticides are recognized as an efficient alternative to synthetic pesticides for pest and disease crop management. However, their commercial production processes use grains, generating large amounts of organic waste, even when agriculture waste or byproducts are the feedstock of choice. Frequently, these organic wastes are rich in nutrients that, after adequate treatment, can be used as nitrogen and carbohydrate sources for secondary metabolite production produced by microorganisms during submerged fermentation. In this sense, this study aimed to prove the concept that biopesticides could be produced under a full biorefinery process, using the entire biomass of an underexplored agroindustrial waste—damaged bean—as the main feedstock. A combination of sequential processes, including solid state fermentation, hydrolysis, and submerged fermentation, were designed for the production of two biopesticides (conventional—fungal conidia and second-generation secondary metabolite—cerulenin) from a high potential biological control agent strain Sarocladium oryzae BRM 59907. The combined processes, using damaged common bean grain as the main feedstock, provided biopesticides and organic fertilizer production that successfully controlled common bean root rot disease. This work proved to be possible the biopesticide production using a full biorefinery concept, inside the same productive chain, contributing to a sustainable environment and economy, together with animal and human health safety.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Data availability
All data generated or analyzed during this study are included in this published article and its supplementary information files.
References
Bills GF, Platas G, Gams W (2004) Conspecificity of the cerulenin and helvolic acid producing `Cephalosporium caerulens’, and the hypocrealean fungus Sarocladium oryzae. Mycol Res 108:1291–1300. https://doi.org/10.1017/S0953756204001297
Blum B, Nicot PC, Kohl J, Ruocco M (2011) Identified difficulties and conditions for field success of biocontrol. Economic aspects: cost analysis. In: Classical and augmentative biological control against diseases and pests: critical status and review of factors influencing their success. IOBC/WPRS, INRA, France, pp 58–61
Bonaterra A, Badosa E, Cabrefiga J, Francés J, Montesinos E (2012) Prospects and limitations of microbial pesticides for control of bacterial and fungal pomefruit tree diseases. Trees (berl West) 26:215–226. https://doi.org/10.1007/s00468-011-0626-y
Bradford MM (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Cerda A, Mejias L, Rodríguez P, Rodríguez A, Artola A, Font X, Gea T, Sánchez A (2019) Valorisation of digestate from biowaste through solid-state fermentation to obtain value added bioproducts: a first approach. Bioresour Technol 271:409–416. https://doi.org/10.1016/j.biortech.2018.09.131
Chaudhary S, Anderson TR, Park SJ, Yu K (2006) Comparison of screening methods or resistance to fusarium root rot in common beans. J Phytopath 154:303–308. https://doi.org/10.1111/j.1439-0434.2006.01097.x
Companhia Nacional de Abastecimento (Conab) https://www.conab.gov.br. Accessed November 2020 (in Portuguese)
Côrtes MVCB, Guimaraes RA, Freire DMG, Prabhu AS, Silva-Lobo VL (2021a) An overview of the virulence factors and the biocontrol potential of Sarocladium oryzae. Fungal Biol Rev 37:1–7. https://doi.org/10.1016/j.fbr.2021.04.001
Côrtes MVCB, Oliveira MIS, Mateus JR, Seldin L, Silva-Lobo VL, Freire DMG (2021b) A pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens. Biol Control 152:104460. https://doi.org/10.1016/j.biocontrol.2020.104460
Das MM, Abdulhameed S (2020) Agro-processing residues for the production of fungal bio-control agents. In: Zakaria Z, Aguilar C, Kusumaningtyas R, Binod P (eds) Valorisation of agro-industrial residues – Volume II: Non-biological approaches. Applied Environmental Science and Engineering for a Sustainable Future. Springer, Cham. https://doi.org/10.1007/978-3-030-39208-6_5
Dimou C, Kopsahelis N, Papadaki A, Papanikolaou S, Kookos IK, Mandala I, Koutinas AA (2015) Wine lees valorization: biorefinery development including production of a generic fermentation feedstock employed for poly(3-hydroxybutyrate) synthesis. Int Food Res J 73:81–87. https://doi.org/10.1016/j.foodres.2015.02.020
Embrapa (2012) Manual de Classificação do Feijão: Procedimentos para classificação do feijão. Instrução normativa Numero 12 de 20 de março de 2008
Froes LO (2012) Emprego da farinha de bandinha de feijão carioca extrusada na formulação de misturas para bolo sem gluten contend farinha de quirera de arroz. Dissertation, Universidade Federal de Goiás (in Portuguese)
Garcia DM, Diaz CH, Artiles YC, Ramos RA, Rubi JA (2003) Characterization of the proteinases secreted by Sarocladium oryzae. Biotecnol Apl 20:170–172
Guimarães RA, Barbosa ET, Silva-Lobo VL, Côrtes MVCB (2018) Formulated Sarocladium oryzae suppress common bean root rot caused by Rhizoctonia solani. Curr Res Environ Appl Mycol 8:69–74. https://doi.org/10.5943/cream/8/1/5
Hanson LE (2003) Biological control of damping-off in sugar beet seedlings with Trichoderma species. Int Instit Beet Res Am Soc Sugar Beet Technol Congr San Ant 1:889–893
Horwitz W (1997) Official methods of analysis of the association of official analytical chemists, 16 ed. Geithersbourg, AOAC
Iwai Y, Awaya J, Kesado T, Yamada H, Ōmura S, Hata T (1973) Selective production of cerulenin by Cephalosporium caerulens KF-140. J Ferment Technol 51:575–581
Lie S (1972) The EBC-ninhydrin method for determination of free alpha amino nitrogen. J I Brewing 79:37–41. https://doi.org/10.1002/j.2050-0416.1973.tb03495.x
Lopes LCM (2010) Determinação das melhores condições de extrusão e caracterização de farinha de feijão para utilização como ingrediente de alimentos instantâneos. Dissertation, Universidade Federal de Goiás (in Portuguese)
Magalhães ALR (2005) Residue from common bean (Phaseolus vulgaris L.) processing in the rations of cattle. Thesis, Universidade Federal de Viçosa (in Portuguese)
Maity SK (2015) Opportunities, recent trends and challenges of integrated biorefinery: Part I. Renew Sust Energ Rev 43:1427–1445. https://doi.org/10.1016/j.rser.2014.11.092
Michaud D, Faye L, Yelle S (1993) Electrophoretic analysis of plant cysteine and serine proteinases using gelatin-containing polyacrylamide gels and class-specific proteinases inhibitors. Electrophoresis 14:783–787. https://doi.org/10.1002/elps.1150140117
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428. https://doi.org/10.1021/ac60147a030
Perret D (2007) From ‘protein’ to the beginnings of clinical proteomics. Proteomics Clin Appl 1:720–738. https://doi.org/10.1002/prca.200700525
Pham TA, Kim JJ, Kim SG, Kim K (2009) Production of blastospore of entomopathogenic Beauveria bassiana in a submerged batch culture. Mycobiology 37(3):218–224. https://doi.org/10.4489/MYCO.2009.37.3.218
Posada-Flórez FJ (2008) Production of Beauveria bassiana fungal spores on rice to control the coffee berry borer, Hypothenemus hampei, in Colombia. J Insect Sci 26:1–13. https://doi.org/10.1673/031.008.4101
Schoonhoven A and Pastor-Corrales MA (1987) Sistema estándar para la evaluacíon de germoplasma de frijol. Centro Internacional de Agricultura Tropical, Cali, Colômbia, 56p. (in Spanish)
Silva JN, Mascarin GM, Gomes ICS, Tinôco RS, Quintela ED, Castilho LR, Freire DMG (2018) New cost-effective bioconversion process of palm kernel cake into bioinsecticides based on Beauveria bassiana and Isaria javanica. Appl Microbiol Biotech 102:2595–2606. https://doi.org/10.1007/s00253-018-8805-z
Trindade DR, Poltronieri LS, Albuquerque FC, Poltronieri MC (1997) Ocorrência do fungo Scytalidium lignicola agente causal da podridão negra do caule e da raiz de mandioca no estado do Pará. Trop Plant Pathol 22:316–331
Tsakona S, Kopsahelis N, Chatzifragkou A, Papanikolaou S, Kookos IK, Koutinas AA (2014) Formulation of fermentation media from flour-rich waste streams for microbial lipid production by Lipomyces starkeyi. J Biotechnol 189:36–45. https://doi.org/10.1016/j.jbiotec.2014.08.011
Tsouko E, Kachrimanidou V, Santos AF, Lima MENV, Papanikolaou S, Castro AM, Freire DMG, Koutinas AA (2017) Valorization of by-products from palm oil mills for the production of generic fermentation media for microbial oil synthesis. Appl Biochem Biotechnol 181:1241–1256. https://doi.org/10.1007/s12010-016-2281-7
Zabaniotou A, Kamaterou P, Kachrimanidou V, Vlysidis A, Koutinas AA (2018) Taking a reflexive TRL3-4 approach to sustainable use of sunflower meal for the transition from a mono-process pathway to a cascade biorefinery in the context of Circular Bioeconomy. J Clean Prod 72:4119–4129. https://doi.org/10.1016/j.jclepro.2017.01.151
Acknowledgements
The authors wish to acknowledge Elder Tadeu Barbosa and Ronair José Pereira for technical support.
Funding
This study was supported by Embrapa—Brazilian Agricultural Research Corporation and Conselho Nacional de Desenvolvimento Científico e Tecnológico – Ministério da Ciência e Tecnologia—Brasil (National Council for Scientific and Technological Development – Ministry of Science and Technology—Brazil) Grant number: CNPq 428610/2018–8.
Author information
Authors and Affiliations
Contributions
Marcio Vinicius de Carvalho Barros Cortes, Valacia Lemes da Silva-Lobo and Denise Maria Guimaraes Freire contributed to the study’s conception and design. Marcio Vinicius de Carvalho Barros Cortes and Maysa Silva Barreto performed the material preparation and data collection. Marcio Vinicius de Carvalho Barros Cortes analyzed the data. The first draft of the manuscript was written by Marcio Vinicius de Carvalho Barros Cortes and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Ethics approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Competing interests
The authors declare no competing interests.
Additional information
Responsible Editor: Ta Yeong Wu
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
de Carvalho Barros Cortes, M.V., Barreto, M.S., da Silva-Lobo, V.L. et al. Sustainable production of biopesticides for common bean root rot control within the biorefinery approach: a Technology Readiness Level 3 experimental proof of concept. Environ Sci Pollut Res 30, 28831–28846 (2023). https://doi.org/10.1007/s11356-022-24252-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-022-24252-6