Abstract
Aquatic worms are considered a suitable group to evaluate the effects of contaminants on the environment, although one of the main challenges is to use the species of local occurrence. Recently, Pristina longiseta was suggested to be used in acute bioassays. In this context, this study aimed to establish a chronic exposure for ecotoxicological bioassays using the cosmopolitan species of occurrence in Brazilian freshwater P. longiseta. Firstly, we tested three exposure times (4, 7, and 10 days) under the presence or absence of aeration for reproduction outputs. After determining the best configuration (7 days without aeration), we assessed the effects of the chronic exposures using the standardized reference substance potassium chloride (KCl), the antibiotic sulfamethoxazole (SMX), the flame retardant tetrabromobisphenol A (TBBPA), and the sugarcane vinasse. Our results showed suitability for applying the chronic exposure using P. longiseta and indicated the sensitivity of the offspring to KCl (EC50-7d = 0.51 g/L). Sulfamethoxazole and TBBPA caused a significant decrease in the offspring of P. longiseta (EC50-7d = 59.9 µg/L and < 62.5 µg/L, respectively). Sugarcane vinasse showed high toxicity for the species, and 4.26% of vinasse was calculated as EC50-7d. Therefore, the described protocol was successfully applied as an ecotoxicological bioassay to evaluate the effects of environmental contaminants on the reproduction rate of the freshwater worm P. longiseta.
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We are grateful to the National Council for Scientific and Technological Development (CNPq), the Brazilian Coordination for the Improvement of Higher Education Personnel (CAPES), and the São Paulo Research Foundation (FAPESP) for financing this research through the award of research grants.
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This study was funded by the National Council for Scientific and Technological Development (CNPq), the Brazilian Coordination for the Improvement of Higher Education Personnel (CAPES), and the São Paulo Research Foundation (FAPESP) process numbers 2020/11042–0 and 2020/09912–6.
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Tallyson Souza, research, experiments, writing, data analyzes, and review; Gleyson Castro, research, experiments, writing, and review; Aline Bernegossi, research, writing, and review; Mayara Felipe, experiments, data analyses, and graphing; Fernanda Pinheiro, research, experiments, review, and cultivation of the species in the laboratory; Vanessa Colombo-Corbi, writing and review; Douglas Girolli, collection and species description; Guilherme Gorni, species description and data analyzes; Juliano Corbi, supervision, writing, and final review.
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de Souza, T.T.C., Castro, G.B., Bernegossi, A.C. et al. Pristina longiseta reproduction test: chronic exposure to environmental contaminants. Environ Sci Pollut Res 30, 23578–23588 (2023). https://doi.org/10.1007/s11356-022-23861-5
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DOI: https://doi.org/10.1007/s11356-022-23861-5