Abstract
Narcissus tazetta (Amaryllidaceae) is a medicinal plant widely used for cut flowers and potted ornamental plant in Tunisia flora. The current study evaluated the phenolic composition and antioxidant properties of its flower extracts and investigated its potential protective activity against cadmium chloride (CdCl2)–induced hepatotoxicity in mice. Mice were divided into six groups of six each: group 1, serving as negative controls, received by intraperitoneal way only distilled water; group 2 received by intraperitoneal way CdCl2 (0.16 mg/kg bw); groups 3 and 4 received CdCl2 at the same dose of group 2 and 100 or 200 mg/kg bw of Narcissus tazetta flower extracts via oral route; groups 5 and 6, serving as positive controls, received only Narcissus tazetta flower extracts. Polyphenolic compounds of the extract were analyzed by colorimetric and high-performance liquid chromatography-mass spectrometry (HPLC-MS) methods. Total antioxidant activity and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging potential of the extract were estimated using colorimetric method. Results indicated that ethanolic flower extract contained high levels of total phenolic and flavonoid along with a strong total antioxidant and DPPH free radical scavenging activities. HPLC-MS analysis identified eight phenolic compounds, including rutin, kaempferol glycosides, and chlorogenic acids. The extract also exhibited marked hepatoprotective effects against CdCl2 toxicity by reducing hepatic levels of malondialdehyde, advanced oxidation protein products, hydrogen peroxide, metallothioneins, and DNA degradation. Additionally, co-administration of Narcissus tazetta flower extracts lowered the plasma activities of transaminases, gamma glutamyl transpeptidase, and lactate dehydrogenase and increased hepatic levels of reduced glutathione, nonprotein thiols, vitamin C, and catalase activity. The hepatoprotective effects of the extract were demonstrated by histopathological improvement of liver disorders. The current study provided ethnopharmacological application of Narcissus tazetta flower extracts against CdCl2-induced oxidative stress, suggesting its chemoprevention role of its phenolic compounds as a natural antioxidant.
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Abbreviations
- AOPP:
-
Advanced oxidation protein products
- AST:
-
Aspartate aminotransferase
- ALT:
-
Alanine aminotransferase
- CdCl2 :
-
Cadmium chloride
- CAT:
-
Catalase
- DPPH:
-
2,2-Diphenyl-1-picrylhydrazyl
- γGT:
-
Gamma glutamyl transpeptidase
- GPx:
-
Glutathione peroxidase
- HPLC:
-
High-performance liquid chromatograph
- H2O2 :
-
Hydrogen peroxide
- LDH:
-
Lactate dehydrogenase
- MDA:
-
Malondialdehyde
- MS:
-
Mass spectrometry
- MTs:
-
Metallothioneins
- NPSH:
-
Nonprotein thiols
- ROS:
-
Reactive oxygen species
- GSH:
-
Reduced glutathione
- SOD:
-
Superoxide dismutase
- Vit C:
-
Vitamin C
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Acknowledgements
The current work was supported by the Ministry of Higher Education and Laboratory of Plant Productivity and Environmental Constraints (LR18ES04). The authors are grateful to Dr. Abdelali Hannoufa, research scientist-adjunct professor at Agriculture and Agri-Food Canada, for critical reading of the manuscript and Mr. Mejri Hssan for his skillful technical assistance.
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This work received financial assistance from the Ministry of Higher Education and Scientific Research of Tunisia.
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SBA and MS were responsible for most of the work; NS contributed to data analysis, AH contributed to the biochemical assays and data analysis; SB contributed to the molecular assays; SBA wrote the manuscript draft; MEL and NKB edited and revised the manuscript and supervised the research project. All authors read and approved the final manuscript.
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Ben-Abdallah, ., Sefi, M., Soudani, N. et al. Potential antioxidant effects of Narcissus tazetta phenolic compounds against cadmium chloride–induced hepatotoxicity in Swiss albino mice. Environ Sci Pollut Res 28, 66193–66205 (2021). https://doi.org/10.1007/s11356-021-15497-8
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DOI: https://doi.org/10.1007/s11356-021-15497-8