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Assessment of actinobacteria use in the elimination of multidrug-resistant bacteria of Ibn Tofail hospital wastewater (Marrakesh, Morocco): a chemometric data analysis approach

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Abstract

The efficiency of the treatment of hospital wastewater by actinobacteria was investigated using two chemometric data analysis methods. Six strains of multi-resistant bacteria isolated from Marrakesh hospital wastewater and four strains of antagonistic actinobacteria isolated from Moroccan marine environment were characterized by fatty acids released as methyl esters by thermochemolysis-GC/MS. The hierarchical cluster analysis (HCA) and the principal component analysis (PCA) were used to correlate fatty acids (FA) distributions within strains. HCA allowed to discriminate between bacteria and actinobacteria. A lower Euclidean distance is noted for bacteria. With PCA, linear and branched-chained FAs correlated with bacteria whereas mono unsaturated FAs correlated more specifically with Gram (−) bacteria. Terminally branched-chained FAs correlated most likely with actinobacteria. A co-culture of actinobacteria and bacteria monitored during 15 days demonstrated the efficiency of the biological treatment for 2 of the 4 studied actinobacteria. The effect is more important on Gram-negative bacteria. Antagonistic actinobacteria seem to be poorly efficient against Gram-positive bacteria.

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Data availability

The datasets used and analyzed during the current study are available from the corresponding authors, upon request.

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Funding

This project was financially supported by the CAMPUS FRANCE (PHC TOUBKAL 2017 (French-Morocco bilateral program, Grant Number: 12345AB)).

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Authors and Affiliations

  1. Laboratory of Microbial Biotechnologies, Agrosciences and Environment (BioMAgE), Faculty of Sciences Semlalia, Cadi Ayyad University, PO Box 2390, Marrakesh, Morocco

    Wassila Korichi & Yedir Ouhdouch

  2. Laboratory of Microbiology and Virology, Faculty of Medicine and Pharmacy, Cadi Ayyad University, PO Box 7010, Marrakesh, Morocco

    Wassila Korichi & Souad Loqman

  3. Institut de Chimie des Milieux et Matériaux de Poitiers (IC2MP - CNRS UMR 7285), Université de Poitiers, 4 rue Michel Brunet – TSA 51106, 86073, Poitiers Cedex 9, France

    Wassila Korichi, Khaled Younes & Laurent Lemée

  4. Laboratoire de Chimie Organique et Analytique, FST, Université Sultan Moulay Slimane, Beni Mellal, Morocco

    Manar Ibrahimi

  5. Agro Bio Sciences Program, Mohammed VI Polytechnic University (UM6P), 43150, Benguerir, Morocco

    Yedir Ouhdouch

  6. College of Engineering and Technology, American University of the Middle East (AUM), Kuwait, Kuwait

    Khaled Younes

Authors
  1. Wassila Korichi
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  2. Manar Ibrahimi
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  3. Souad Loqman
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  4. Yedir Ouhdouch
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  5. Khaled Younes
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  6. Laurent Lemée
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Contributions

WK: strain isolation and performing the molecular analysis. MI: assisting in strain isolation. SL: assisting in strain isolation and reviewing the article. YO: assisting in strain isolation and reviewing the article. KY: interpreting and discussing results and writing the manuscript. LL: assisting in the molecular analysis, reviewing the article, and coordinating the project.

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Correspondence to Khaled Younes or Laurent Lemée.

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Responsible Editor: Diane Purchase

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Korichi, W., Ibrahimi, M., Loqman, S. et al. Assessment of actinobacteria use in the elimination of multidrug-resistant bacteria of Ibn Tofail hospital wastewater (Marrakesh, Morocco): a chemometric data analysis approach. Environ Sci Pollut Res 28, 26840–26848 (2021). https://doi.org/10.1007/s11356-021-12445-4

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  • DOI: https://doi.org/10.1007/s11356-021-12445-4

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