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Rhizosphere microbiome dynamics in tropical seagrass under short-term inorganic nitrogen fertilization

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Abstract

Rhizosphere microbes are crucial to seagrass meadows because they promote plant growth and heath. However, information concerning the response of rhizosphere microorganisms in seagrass sediment in the presence of different nitrogen sources is lacking. Here, by means of high-throughput sequencing, we investigated how addition of inorganic nitrogen affects the rhizosphere microbiome of the tropical seagrass Thalassia hemperichii. A seagrass culture system was set up to conduct a nitrogen addition (ammonium and nitrate) simulation experiment. We found that the relative abundance of Proteobacteria and Bacteroidetes was increased in inorganic nitrogen-enriched samples, whereas that of Acidobacteria decreased under ammonium enrichment, especially after 35 days. High levels of inorganic nitrogen addition caused a significant decrease in the relative abundance of Desulfobacteraceae, Sulfurovaceae, and Spirochaetes, which are primarily involved in sulfur cycling. Additionally, the abundance of microbes in the seagrass rhizosphere reached the highest after the ammonium-enrichment treatment. Among the analyzed seagrass photosynthetic characteristics, seagrass leaves presented the highest light utility in treatments receiving nitrate, followed by the control groups and ammonium-enrichment groups. Moreover, 16S rRNA gene-predicted functional analysis suggested that some functions related to metabolism of amino acids and signal transduction were enriched in samples receiving high ammonium, whereas nitrate addition enriched predicted functions related to diseases. These findings provide new insights into the response of microbial communities to different types of nitrogen additions in seagrass ecosystems.

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Acknowledgments

The authors would like to thank all of the members of Tropical Marine Biological Research Station in Hainan for their helping work in sample collecting.

Funding

This research was financially supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA13020300), the National Natural Science Foundation of China (41676163, 41406191, 41676107 and 41976147), Pearl River S&T Nova Program of Guangzhou (201806010017), Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0402), National Key Research and Development Program of China (2018YFC1406505, 2017YFC0506301 and 2018FY100105), Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences (ISEE2018ZD02), the Guangdong Province Public Welfare Research and Capacity Building Project (2015A020216016) and the Science and Technology Planning Project of Guangdong Province, China (2017B030314052).

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Authors and Affiliations

  1. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Weiguo Zhou, Junde Dong, Dewen Ding, Lijuan Long, Anning Suo, Xiancheng Lin, Qingsong Yang, Liyun Lin, Yanying Zhang & Juan Ling

  2. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458, Guangdong Province, China

    Weiguo Zhou, Junde Dong, Dewen Ding, Lijuan Long, Anning Suo, Qingsong Yang & Juan Ling

  3. Tropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, Sanya, 572000, China

    Junde Dong & Yanying Zhang

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  1. Weiguo Zhou
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  10. Juan Ling
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Contributions

Weiguo Zhou, Juan Ling conceived and designed the study. Weiguo Zhou collected the sample and set up a seagrass culture system with the help of Qingsong Yang and Xiancheng Lin. Junde Dong, Dewen Ding, Lijuan Long, Anning Suo, Liyun Lin, Yanying Zhang, and Juan Ling contributed to laboratory analysis and interpretation of the results. Weiguo Zhou drafted the manuscript with input from all the authors.

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Correspondence to Juan Ling.

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Zhou, W., Dong, J., Ding, D. et al. Rhizosphere microbiome dynamics in tropical seagrass under short-term inorganic nitrogen fertilization. Environ Sci Pollut Res 28, 19021–19033 (2021). https://doi.org/10.1007/s11356-020-12048-5

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