Skip to main content
SpringerLink
Log in

Effect of diosmin on lipid peoxidation and organ damage against subacute deltamethrin exposure in rats

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

The aim of this study was to investigate the protective efficacy of diosmin against subacute deltamethrin exposure. For this purpose, 40 male Wistar albino rats were used. The animals were assigned to the following 4 groups: control group (received corn oil vehicle alone), diosmin-treated group (50 mg/kg bw/day orally), deltamethrin-exposed group (5 mg/kg bw/day, orally) and coadministered group (5 mg/kg bw/day deltamethrin and 50 mg/kg bw/day diosmin, orally) for 28 days. Some lipid peroxidation/antioxidant status/biochemical markers were evaluated in blood/tissue (liver, kidney, brain, heart and testis) samples and the histopathological architecture was assessed. Compared with the control group, no alteration was detected in the parameters and histological findings of the diosmin-treated group. Deltamethrin toxicity was associated with significantly increased plasma, cardiac, hepatic, renal, cerebral and testicular levels of MDA and NO, and significantly decreased GSH levels (p < 0.05). Antioxidant enzyme status (SOD, CAT and GSH-Px activities) displayed either decrease or increase (p < 0.05). Significant increase was detected in AST and ALT activities and urea and creatinine levels (p < 0.05). The values of the group coadministered with deltamethrin and diosmin were similar to the values of the control group. Diosmin ameliorated deltamethrin-induced lymphocytic and histiocytic infiltration and subendocardial oedema in the heart. Combined administration also minimized hepatic, renal, testicular and cerebral histopathological findings. The alterations detected in various toxicological parameters correlated well with the histopathological changes observed in various organs. In conclusion, it is suggested that diosmin could provide protection against deltamethrin-induced toxicity and organ damage in rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

Data availability

The data obtained after analysis in the current study can be obtained from the relevant authors upon reasonable request.

References

  • Abdallah FB, Hamden K, Galeraud-Denis I, El Feki A, Keskes-Ammar L (2010) An in vitro study on reproductive toxicology of deltamethrin on rat spermatozoa. Andrologia 42:254–259

    Google Scholar 

  • Abdel-Daim MM, Abuzead SMM, Halawa SM (2013) Protective role of Spirulina platensis against acute deltamethrin-induced toxicity in rats. PLoS One 8:e72991

    CAS  Google Scholar 

  • Abdel-Daim MN, Abd Eldaim MA, Mahmoud MM (2014) Trigonella foenum-graecum protection against deltamethrin-induced toxic effects on haematological, biochemical, and oxidative stress parameters in rats. Can J Physiol Pharmacol 92:679–685

    CAS  Google Scholar 

  • Abdel-Daim MM, Abdelkhalek NKM, Hassan AM (2015) Antagonistic activity of dietary allicin against deltamethrin-induced oxidative damage in freshwater Nile Tilapia; Oreochromis Niloticus. Ecotoxicol Environ Saf 111:146–152

    CAS  Google Scholar 

  • Abdel-Daim M, El-Bialy BE, Rahman HGA, Radi AM, Hefny HA, Hassan AM (2016) Antagonistic effects of Spirulina platensis against sub-acute deltamethrin toxicity in mice: biochemical and histopathological studies. Biomed Pharmacother 77:79–85

  • Abdel-Daim MM, Khalifa HA, Abushouk AI, Dkhil MA, Al-Quraishy SA (2017) Diosmin attenuates methotrexate-induced hepatic, renal, and cardiac injury: A biochemical and histopathological study in mice. Oxid Med Cell Longev 3281670. Oxid Med Cell Longev 3281670, doi: https://doi.org/10.1155/2017/3281670

  • Abdel-Daim MM, Khalil SR, Awad A, Abu Zeid EH, Abd El-Aziz R, El-Serehy HA (2020) Ethanolic extract of Moringa oleifera leaves influences NF-κB signaling pathway to restore kidney tissue from cobalt-mediated oxidative injury and inflammation in rats. Nutrients 12:1031. https://doi.org/10.3390/nu12041031

  • Abdelhamid FM, Mahgoub HA, Ateya AI (2020) Ameliorative effect of curcumin against lead acetate-induced hemato-biochemical alterations, hepatotoxicity, and testicular oxidative damage in rats. Environ Sci Pollut Res Int 27:10950–10965

    CAS  Google Scholar 

  • Ahmed S, Mundhe N, Borgohain M, Chowdhury L, Kwatra M, Bolshette N, Ahmed A, Lahkar M (2016) Diosmin modulates the NF-kB signal transduction pathways and downregulation of various oxidative stress markers in alloxan-induced diabetic nephropathy. Inflammation 39:1783–1797

    CAS  Google Scholar 

  • Ali SS, Ahsan H, Zia MK, Siddiqui T, Khan FH (2020a) Understanding oxidants and antioxidants: classical team with new players. J Food Biochem 44:e13145

    Google Scholar 

  • Ali TM, Abo-Salem OM, El Esawy BH, El Askary A (2020b) The potential protective effects of diosmin on streptozotocin induced diabetic cardiomyopathy in rats. Am J Med Sci 359:32–41

    Google Scholar 

  • Arora D, Siddiqui MH, Sharma PK, Singh SP, Tripathi A, Mandal P, Singh US, Singh PK, Shukla Y (2016) Evaluation and physiological correlation of plasma proteomic fingerprints for deltamethrin-induced hepatotoxicity in Wistar rats. Life Sci 160:72–83

    CAS  Google Scholar 

  • Bergqvist D, Hallböök T, Lindblad B, Lindhagen A (1981) A double-blind trial of O-(beta-hydroxyethyl)-rutoside in patients with chronic venous insufficiency. Vasa 10:253–260

    CAS  Google Scholar 

  • Biswas SK (2016) Does the interdependence between oxidative stress and inflammation explain the antioxidant paradox? Oxid Med Cell Longev, https://doi.org/10.1155/2016/5698931

  • Bozdağ M, Eraslan G (2020) The effect of diosmin against lead exposure in rats. Naunyn Schmiedeberg's Arch Pharmacol 393:639–649

    Google Scholar 

  • Camarda L, Di Stefano V, Del Bosco SF, Schillaci D (2007) Antiproliferative activity of Citrus juices and HPLC evaluation of their flavonoid composition. Fitoterapia 78:426–429

    CAS  Google Scholar 

  • Chargui I, Grissa I, Bensassi F, Hrira MY, Haouem S, Haouas Z, Bencheikh H (2012) Oxidative stress, biochemical and histopathological alterations in the liver and kidney of female rats exposed to low doses of deltamethrin (DM): a molecular assessment. Biomed Environ Sci 25:672–683

    CAS  Google Scholar 

  • Chrustek A, Hołyńska-Iwan I, Dziembowska I, Bogusiewicz J, Wróblewski M, Cwynar A, Olszewska-Słonina D (2018) Current research on the safety of pyrethroids used as insecticides. Medicina (Kaunas) 54:61. https://doi.org/10.3390/medicina54040061

    Article  Google Scholar 

  • Croft KD (1998) The chemistry and biological effects of flavonoids and phenolic acids. Ann N Y Acad Sci 854:435–442

    CAS  Google Scholar 

  • Dubey N, Khan AM, Raina R (2013) Sub-acute deltamethrin and fluoride toxicity induced hepatic oxidative stress and biochemical alterations in rats. Bull Environ Contam Toxicol 91:334–338

    CAS  Google Scholar 

  • Ensley SM (2012) Pyrethrins and Pyretroids. In: Gupta RC (ed) Veterinary toxicology, basic and clinical principles, 2nd edn. Academic Press, San Diego, pp 591–595

    Google Scholar 

  • Eraslan G, Sarıca ZS, Bayram LÇ, Tekeli MY, Kanbur M, Karabacak M (2017) The effects of diosmin on aflatoxin-induced liver and kidney damage. Environ Sci Pollut Res Int 24:27931–27941

    CAS  Google Scholar 

  • Fairbanks VF, Klee GG (1987) Biochemical aspect of hematology. In: Tietz NW (ed) Fundamentals of clinical chemistry, 3rd Edn. WB Saunders, Philadelphia, pp 803–806

    Google Scholar 

  • Fujita T (2002) Formation and removal of reactive oxygen species, lipid peroxides and free radicals, and their biological effects. Yakugaku Zasshi 122:203–218

    CAS  Google Scholar 

  • Gibson-Corley KN, Olivier AK, Meyerholz DK (2013) Principles for valid histopathologic scoring in research. Vet Pathol 50:1007–1015

    CAS  Google Scholar 

  • Gutteridge JMC, Halliwell B (2018) Mini-Review: Oxidative stress, redox stress or redox success? Biochem Biophys Res Commun 502:183–186

    CAS  Google Scholar 

  • Halliwell B (2012) Free radicals and antioxidants: updating a personal view. Nutr Rev 70:257–265

    Google Scholar 

  • Issam C, Samir H, Zohra H, Monia Z, Hassen BC (2009) Toxic responses to deltamethrin (DM) low doses on gonads, sex hormones and lipoperoxidation in male rats following subcutaneous treatments. J Toxicol Sci 34:663–670

    CAS  Google Scholar 

  • Khalil SR, Khalif HA, Abdel-Motal SM, Mohammed HH, Elewad YHA, Mahmou HA (2018a) Spirulina platensis attenuates the associated neurobehavioral and inflammatory response impairments in rats exposed to lead acetate. Ecotoxicol Environ Saf 157:255–265

    CAS  Google Scholar 

  • Khalil SR, Elhady WM, Elewa YHA, El-Hameed NEA, Ali SA (2018b) Possible role of Arthrospira platensis in reversing oxidative stress-mediated liver damage in rats exposed to lead. Biomed Pharmacother 97:1259–1268

    CAS  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Lu Q, Sun Y, Ares I, Anadón A, Martínez M, Martínez-Larrañaga MR, Yuan Z, Wang X, Martínez MA (2019) Deltamethrin toxicity: a review of oxidative stress and metabolism. Environ Res 170:260–281

    CAS  Google Scholar 

  • Luck H (1965) Catalase. In: Bergmeyer H (ed) Methods of enzymatic analysis. Academic Press, New York, pp 885–894

    Google Scholar 

  • Mahgoub S, Sallam AO, Sarhan HKA, Ammar AAA, Soror SH (2020) Role of diosmin in protection against the oxidative stress induced damage by gamma-radiation in Wistar albino rats. Regul Toxicol Pharmacol 113:104622. https://doi.org/10.1016/j.yrtph.2020.104622

  • Mann PC, Vahle J, Keenan CM, Baker JF, Bradley AE, Goodman DG, Harada T, Herbert R, Kaufmann W, Kellner R, Nolte T, Rittinghausen S, Tanaka T (2012) International harmonization of toxicologic pathology nomenclature: an overview and review of basic principles. Toxicol Pathol 40:7S–13S

    Google Scholar 

  • Miller GL (1959) Protein determination for large numbers of samples. Anal Chem 31:964–964

    CAS  Google Scholar 

  • Mirzaee S, Mansouri E, Shirani M, Zeinvand-Lorestani M, Khodayar MJ (2019) Diosmin ameliorative effects on oxidative stress and fibrosis in paraquat-induced lung injury in mice. Environ Sci Pollut Res Int 26:36468–36477

    CAS  Google Scholar 

  • Mohafrash SMM, Mossa ATH (2020) Herbal syrup from chicory and artichoke leaves ameliorate liver damage induced by deltamethrin in weanling male rats. Environ Sci Pollut Res Int 27:7672–7682

  • Mohammadi H, Ghassemi-Barghi N, Malakshah O, Ashari S (2019) Pyrethroid exposure and neurotoxicity: a mechanistic approach. Arh Hig Rada Toksikol 70:74–89

    CAS  Google Scholar 

  • Ncir M, Saoudi M, Sellami H, Rahmouni F, Lahyani A, Ayadi FM, El Feki A, Allagui MS (2018) In vitro and in vivo studies of Allium sativum extract against deltamethrin-induced oxidative stress in rats brain and kidney. Arch Physiol Biochem 124:207–217

  • Ohkawa H, Ohishi N, Yagi K (1978) Reaction of linoleic acid hydroperoxide with thiobarbituric acid. J Lipid Res 19:1053–1057

    CAS  Google Scholar 

  • Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    CAS  Google Scholar 

  • Peduto VA, D'Uva R, Piga M (1996) Carbamate and organophosphate poisoning. Minerva Anestesiol 62:33–54

    CAS  Google Scholar 

  • Poljsak B, Šuput D, Milisav I (2013) Achieving the balance between ros and antioxidants: when to use the synthetic antioxidants. Oxid Med Cell Longev, 956792, doi: https://doi.org/10.1155/2013/956792

  • Ray DE, Forshaw PJ (2000) Pyrethroid insecticides: poisoning syndromes, synergies, and therapy. J Toxicol Clin Toxicol 38:95–101

    CAS  Google Scholar 

  • Rehman H, Mohan A, Tabassum H, Ahmad F, Rahman S, Parvez S, Raisuddin S (2011) Deltamethrin increases Candida albicans infection susceptibility in mice. Scand J Immunol 73:459–464

    CAS  Google Scholar 

  • Rjeibi I, Ben Saad A, Hfaiedh N (2016) Oxidative damage and hepatotoxicity associated with deltamethrin in rats: the protective effects of Amaranthus spinosus seed extract. Biomed Pharmacother 84:853–860

    CAS  Google Scholar 

  • Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205

    CAS  Google Scholar 

  • Shalkami AS, Hassan M, Bakr AG (2018) Anti-inflammatory, antioxidant and anti-apoptotic activity of diosmin in acetic acid-induced ulcerative colitis. Hum Exp Toxicol 37:78–86

    CAS  Google Scholar 

  • Sharma P, Singh R, Jan M (2014) Dose-dependent effect of deltamethrin in testis, liver, and kidney of Wistar rats. Toxicol Int 21:131–139

    Google Scholar 

  • Sharmila Queenthy S, Stanely Mainzen Prince P, John B (2018) Diosmin prevents isoproterenol-induced heart mitochondrial oxidative stress in rats. Cardiovasc Toxicol 18:120–130

    CAS  Google Scholar 

  • Silambarasan T, Raja B (2012) Diosmin, a bioflavonoid reverses alterations in blood pressure, nitric oxide, lipid peroxides and antioxidant status in DOCA-salt induced hypertensive rats. Eur J Pharmacol 679:81–89

    CAS  Google Scholar 

  • Slaninova A, Smutna M, Modra H, Svobodova Z (2009) A review: oxidative stress in fish induced by pesticides. Neuro Endocrinol Lett 30:2–12

    CAS  Google Scholar 

  • Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500

  • Tahir M, Rehman MU, Lateef A, Khan R, Khan AQ, Qamar W, Ali F, O'Hamiza O, Sultana S (2013) Diosmin protects against ethanol-induced hepatic injury via alleviation of inflammation and regulation of TNF-α and NF-κB activation. Alcohol 47:131–139

    CAS  Google Scholar 

  • Tanrikulu Y, Şahin M, Kismet K, Kilicoglu SS, Devrim E, Tanrikulu CS, Erdemli E, Erel S, Bayraktar K, Akkus MA (2013) The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental study. Bosn J Basic Med Sci 13:218–224

    Google Scholar 

  • Tewari A, Banga HS, Gill J (2018) Sublethal chronic effects of oral dietary exposure to deltamethrin in Swiss albino mice. Toxicol Ind Health 34:423–432

    CAS  Google Scholar 

  • Tracey WR, Tse J, Carter G (1995) Lipopolysaccharide-induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. J Pharmacol Exp Ther 272:1011–1015

    CAS  Google Scholar 

  • Tuzmen N, Candan N, Kaya E, Demiryas N (2008) Biochemical effects of chlorpyrifos and deltamethrin on altered antioxidative defense mechanisms and lipid peroxidation in rat liver. Cell Biochem Funct 26:119–124

    CAS  Google Scholar 

  • Uchendu C, Ambali SF, Ayo JO, Esievo KAN, Umosen AJ (2014) Erythrocyte osmotic fragility and lipid peroxidation following chronic co-exposure of rats to chlorpyrifos and deltamethrin, and the beneficial effect of alpha-lipoic acid. Toxicol Rep 1:373–378

  • Varol S, Özdemir HH, Çevik MU, Altun Y, Ibiloğlu I, Ekinci A, Ibiloğlu AO, Balduz M, Arslan D, Tekin R, Aktar F, Aluçlu MU (2016) Protective effects of L-glutamine against toxicity of deltamethrin in the cerebral tissue. Neuropsychiatr Dis Treat 12:1005–1011

    CAS  Google Scholar 

  • Villa P, Cova D, De Francesco L, Guaitani A, Palladini G, Perego R (1992) Protective effect of diosmetin on in vitro cell membrane damage and oxidative stress in cultured rat hepatocytes. Toxicology 73:179–189

    CAS  Google Scholar 

  • Wagner SL (1997) Diagnosis and treatment of organophosphate and carbamate intoxication. Occup Med 12:239–249

    CAS  Google Scholar 

  • WHO (2017) Deltamethrin. WHO specifications for public health pesticides. WHO Specification 333/TC, 1–147

  • Winterbourn CC, Hawkins RE, Brian M, Carrell RW (1975) The estimation of red cell superoxide dismutase activity. J Lab Clin Med 85:337–341

    CAS  Google Scholar 

  • Yoshioka T, Kawada K, Shimada T, Mori M (1979) Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol 135:372–376

  • Yousef MI, Awad TI, Mohamed EH (2006) Deltamethrin-induced oxidative damage and biochemical alterations in rat and its attenuation by vitamin E. Toxicology 227:240–247

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey

    Muhammet Yasin Tekeli & Gökhan Eraslan

  2. Department of Pathology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey

    Latife Çakır Bayram

  3. Hakan Çetinsaya Experimental and Clinical Research Center, Erciyes University, Kayseri, Turkey

    Zeynep Soyer Sarıca

Authors
  1. Muhammet Yasin Tekeli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gökhan Eraslan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Latife Çakır Bayram
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zeynep Soyer Sarıca
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Gökhan Eraslan and Muhammet Yasin Tekeli planned to work by performing literature reviews. The animal experiment stage of the study was carried out by Zeynep Soyer Sarıca and Muhammet Yasin Tekeli. Lipid peroxidation/antioxidant status parameters analysis of the samples taken at the end of the experiment was carried out by Muhammet Yasin Tekeli and Gökhan Eraslan. Histopathological evaluations were performed by Latife Çakır Bayram. The manuscript was prepared by Gökhan Eraslan. All authors have read and approved the manuscript.

Corresponding author

Correspondence to Gökhan Eraslan.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The animal testing procedures followed in this study were approved by the Animal Experiments Board of Erciyes University (decision date: 10.January.2018 and decision no: 18/014).

Consent to participate

All authors contributed voluntarily to this study.

Consent to publish

All authors have consent for the publication of the manuscript.

Additional information

Responsible Editor: Mohamed M. Abdel-Daim

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tekeli, M.Y., Eraslan, G., Çakır Bayram, L. et al. Effect of diosmin on lipid peoxidation and organ damage against subacute deltamethrin exposure in rats. Environ Sci Pollut Res 28, 15890–15908 (2021). https://doi.org/10.1007/s11356-020-11277-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-020-11277-y

Keywords

Search

Navigation