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Raising the water temperature: consequences in behavior and biochemical biomarkers of the freshwater crab Aegla longirostri (Crustacea, Anomura)

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Abstract

Understanding how temperature alone affects biomarkers commonly used in ecotoxicology studies and biomonitoring programs is important to obtain a more real response in field studies, especially in freshwater. Thus, we analyzed the behavioral responses, the lethality, and the biochemical biomarkers in the freshwater crustacean Aegla longirostri at different water temperatures. Animals were exposed under laboratory conditions, to 18 °C, 21 °C, 24 °C, and 26 °C for 48 h. There were significant changes in biochemical parameters in different tissues (hepatopancreas, gills, and muscle) and in the behavioral tests in A. longirostri. Hepatopancreas was especially affected by the elevation of temperature, as showed by the high levels of carbonyl proteins. The activity of acetylcholinesterase increased in a temperature-dependent manner in muscle. Glutathione S-transferase activity decreased with the elevation of temperature in all tissues sampled. The results obtained in this study indicate that when assessing the health of polluted limnic ecosystems through the use of organisms in situ, the intrinsic effect of abiotic factors, such as temperature, on biomarkers must be considered.

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Acknowledgments

We thank Dr. Luis Esteban Krause Lanés, Dr. Charlene Menezes, and Dr. Bernardo Baldisserotto, by suggestions in the manuscript. We also thank Fernando Cereser, Charles Zarantonelo, and laboratory colleagues for the technical support.

Funding

The Brazilian government financially supported this research through CAPES foundation (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) by the grant to CC and CNPq by the research grant to SS (proc. n° 311142/2014-1).

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Authors and Affiliations

  1. Programa de Pós-Graduação em Biodiversidade Animal, Departamento de Ecologia e Evolução, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Cristina Cerezer, Aline Monique Blank do Amaral, Aline Teixeira Marins, Vania Lucia Loro, Marlise Ladvocat Bartholomei-Santos & Sandro Santos

  2. Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Jossiele Wesz Leitemperger & Vania Lucia Loro

  3. Curso em Ciências Biológicas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Bruna Ceretta Ferreira

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  1. Cristina Cerezer
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  2. Jossiele Wesz Leitemperger
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  3. Aline Monique Blank do Amaral
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Correspondence to Cristina Cerezer.

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Cerezer, C., Leitemperger, J.W., do Amaral, A.M.B. et al. Raising the water temperature: consequences in behavior and biochemical biomarkers of the freshwater crab Aegla longirostri (Crustacea, Anomura). Environ Sci Pollut Res 27, 45349–45357 (2020). https://doi.org/10.1007/s11356-020-10423-w

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