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Toxicity assessments of acrylamide in aquatic environment using two algae Nitzschia closterium and Scenedesmus quadricauda

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Abstract

The expanding production and widespread application of acrylamide caused inevitable release to aquatic ecosystems. Contrary to its extensive attention to human and animal health, the hazards of acrylamide to the aquatic primary productivity have been rarely studied. The potential effects of acrylamide on the marine algae (Nitzschia closterium) and the limnetic algae (Scenedesmus quadricauda) were investigated by monitoring cell abundance, total chlorophyll content, maximum photosystem II (PSII) quantum yield (Fv/Fm), and reactive oxygen species (ROS). The growth of two algae was significantly inhibited by acrylamide. The 96 h EC50 of acrylamide on N. closterium and S. quadricauda were 5.50 mg L−1 and 45.3 mg L−1, and no observed effect concentration (NOEC) were 1.07 mg L−1 and 6.97 mg L−1, respectively. After 96 h exposure to 50 mg L−1 of acrylamide, the total chlorophyll content declined to approximate 18% (N. closterium) and 48% (S. quadricauda), and Fv/Fm was observed to be 0.35 and 0.32 for N. closterium and S. quadricauda, respectively. ROS was significantly increased following higher exposure concentrations, and its levels increased around 2.1-fold and 1.4-fold following exposure to 5 mg L−1 of acrylamide. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed the visible cell plasmolysis, rupture of the plasma membrane, cell vacuolization, and disintegration of chloroplasts of the algae caused acrylamide.

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Funding

The study was financially supported by the National Key Research and Development Program (grant number 2016YFC1402101), the National Natural Science Foundation of China (grant number 41876078), the Shandong Provincial Natural Science Foundation of China (grant number ZR2018MD016), and the Special Fund for Basic Scientific Research in Central Colleges and Universities (grant number 201713059).

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Authors and Affiliations

  1. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China

    Ting Zhao & Jiangtao Wang

  2. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, China

    Wenjing Xu, Liju Tan, Xiaolin Zhu & Jiangtao Wang

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  1. Wenjing Xu
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  2. Liju Tan
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  3. Ting Zhao
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  4. Xiaolin Zhu
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Correspondence to Liju Tan or Jiangtao Wang.

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Responsible Editor: Gangrong Shi

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Xu, W., Tan, L., Zhao, T. et al. Toxicity assessments of acrylamide in aquatic environment using two algae Nitzschia closterium and Scenedesmus quadricauda. Environ Sci Pollut Res 27, 20545–20553 (2020). https://doi.org/10.1007/s11356-020-08581-y

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