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Biodegradation of kraft lignin by newly isolated Klebsiella pneumoniae, Pseudomonas putida, and Ochrobactrum tritici strains

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Abstract

Bacterial systems have drawn an increasing amount of attention on lignin valorization due to their rapid growth and powerful environmental adaptability. In this study, Klebsiella pneumoniae NX-1, Pseudomonas putida NX-1, and Ochrobactrum tritici NX-1 with ligninolytic potential were isolated from leaf mold samples. Their ligninolytic capabilities were determined by measuring (1) the cell growth on kraft lignin as the sole carbon source, (2) the decolorization of kraft lignin and lignin-mimicking dyes, (3) the micro-morphology changes and transformations of chemical groups in kraft lignin, and (4) the ligninolytic enzyme activities of these three isolates. To the best of our knowledge, this is the first report that Ochrobactrum tritici species can depolymerize and metabolize lignin. Moreover, laccase, lignin peroxidase, and Mn-peroxidase showed high activities in P. putida NX-1. Due to their excellent ligninolytic capabilities, these three bacteria are important supplements to ligninolytic bacteria library and could be valuable in lignin valorization.

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Acknowledgements

This work was supported by the National Key R&D Program of China (grant number 2016YFE0105400), National Natural Science Foundation of China (grant number 21606132), Natural Science Foundation of Jiangsu Province (grant numbers BK20160823 and BK20170829), and Fundamental Research Funds for the Central Universities (grant numbers 30916011202 and 30917011307).

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  1. School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China

    Zhaoxian Xu, Ling Qin, Mufeng Cai, Wenbo Hua & Mingjie Jin

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  1. Zhaoxian Xu
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  2. Ling Qin
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  3. Mufeng Cai
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  4. Wenbo Hua
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  5. Mingjie Jin
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Correspondence to Mingjie Jin.

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Xu, Z., Qin, L., Cai, M. et al. Biodegradation of kraft lignin by newly isolated Klebsiella pneumoniae, Pseudomonas putida, and Ochrobactrum tritici strains. Environ Sci Pollut Res 25, 14171–14181 (2018). https://doi.org/10.1007/s11356-018-1633-y

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