Abstract
Bacillus sp. CL18 was investigated to propose a bioprocess for protease production using feathers as organic substrate. In feather broth (FB), containing feathers as sole organic substrate (1–100 g l−1), maximal protease production was observed at 30 g l−1 (FB30) after 6 days of cultivation, whereas increased feather concentrations negatively affected protease production and feather degradation. Protease production peaks were always observed earlier during cultivations than maximal feather degradation. In FB30, 80% of initial feathers mass were degraded after 7 days. Addition of glucose, sucrose, starch, yeast extract (2 g l−1), CaCl2, or MgCl2 (10 mmol l−1) to FB30 decreased protease production and feather degradation. FB30 supplementation with NH4Cl (1 g l−1) resulted in less apparent negative effects on protease production, whereas peptone (2 g l−1) increased protease yields earlier during cultivations (3 days). Through a central composite design employed to investigate the effects of peptone and NH4Cl (0.5–4.5 g l−1) on protease production and feather degradation, FB30 supplementation with peptone and NH4Cl (0.5–1.1 g l−1) increased protease production within a shorter cultivation time (5 days) and hastened complete feather degradation (6 days). Feather bioconversion concurs with sustainable production of value-added products.
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Authors thank the “Programa Institucional de Iniciação Científica da Universidade Federal da Fronteira Sul (PRO-ICT/UFFS)” and “Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (PROBIC/FAPERGS).”
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Responsible editor: Santiago V. Luis
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Sobucki, L., Ramos, R.F. & Daroit, D.J. Protease production by the keratinolytic Bacillus sp. CL18 through feather bioprocessing. Environ Sci Pollut Res 24, 23125–23132 (2017). https://doi.org/10.1007/s11356-017-9876-6
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DOI: https://doi.org/10.1007/s11356-017-9876-6