Abstract
A lead-resistant bacterial strain was isolated from coal mine dump and identified as Acinetobacter junii Pb1 on basis of 16S rRNA (ribosomal ribonucleic acid) gene sequencing. The minimum inhibitory concentration of lead for the strain was 16,000 mg l−1 and it showed antibiotic and multi metal resistance. In aqueous culture, at an initial lead (Pb(II)) concentration of 100 and 500 mg l−1, lead adsorption and accumulation by the isolate was 100 and 60%, at pH 7 at 30 °C after 48 and 120 h, respectively. The two fractions of exopolysaccharide (EPS), loosely associated EPS (laEPS) and bound EPS (bEPS), and whole cells (devoid of EPS) showed high binding affinity towards Pb(II). The binding affinity of laEPS towards Pb(II) (1071 mg Pb g−1) was three times higher than that of bEPS (321.5 mg Pb g−1) and 6.5 times higher than that of whole cells (165 mg Pb g−1). The binding affinity of EPS and whole cells with Pb(II), reported in the current study, is considerably higher as compared to that reported in the literature, till date. SEM analysis, showed an increase in thickness of cells on exposure to Pb(II) and TEM analysis, revealed its accumulation (interior of cell) and its adsorption (with the external cell surface). The isolate was also found to be positive for indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylate (ACC) deaminase production which helps in promoting plant growth. Thus, this study provides a new understanding towards Pb(II) uptake by A. junii Pb1, highlighting its potential on the restoration of Pb(II) contaminated repositories.
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References
Bhattacharya A, Gupta A (2013) Evaluation of Acinetobacter sp. B9 for Cr(VI) resistance and detoxification with potential application in bioremediation of heavy-metals-rich industrial wastewater. Environ Sci Pollut Res 6628-6637
Biolog (2002) Microlog user’s manual, release 4.2. Biolog Inc., Hayward
Dhakephalkar PK, Chopade BA (1994) High levels of multiple metal resistance and its correlation to antibiotic resistance in environmental isolates of Acinetobacter. Biometals 7(1):67–74
Gordon, SA, Weber, RP (1951) Colorimetric estimation of indole acetic acid. Plant Physiol. 26:192–195
Gordon JN, Taylor A, Bennett PN (2002) Lead poisoning: case studies. Br J Clin Pharmacol 53:451–458
Guibaud G, Hullebusch E, Bordas F (2006) Lead and cadmium biosorption by extracellular polymeric substances (EPS) extracted from activated sludges: pH-sorption edge tests and mathematical equilibrium modelling. Chemosphere 64:1955–1962
Honma M, Shimomura T (1978) Metabolism of 1-aminocyclopropane- 1-carboxylic acid. Agri. Biol.Chem. 42:1825–1831
Jaafar R, Al-Sulami A, Al-Taee A, Aldoghachi F, Napes S (2015) Biosorption and bioaccumulation of some heavy metals by Deinococcus radiodurans isolated from soil in Basra governorate—Iraq. J Biotechnol Biomater 5:190
Jaafar R, Al-Sulami A, Al-Taee A (2016) Bioaccumulation of cadmium and lead by Shewanella oneidensis isolated from soil in Basra governorate. Iraq Afr J Microbiol Res 10(12):370–375
Jensen V (1951) Notes on the biology of Azotobacter. Proc Soc Appl Bacteriol 74:98–99
Loaёc M, Olier R, Guezennec J (1997) Uptake of lead, cadmium and zinc by a novel bacterial exopolysaccharide. Water Res 31(5):1171–1179
Kim SU, Cheong YH, Seo DC, Hur JS, Heo JS, Cho JS (2007) Characterisation of heavy metal tolerance and biosorption capacity of bacterium strain CPC4 (Bacillus spp.) Water SciTechnol 55(1–2):105–111
Magaldi S, Mata-Essayag S, De Capriles CH, Perez C, Colella MT, Olaizola C, Ontiveros Y (2004) Well diffusion for antifungal susceptibility testing. Int J Infect Dis 8(1):39–45
Masood F, Malik A (2015) Single and multi-component adsorption of metal ions by Acinetobacter sp. FM4. Sep Sci Technol 50:892–900
Naik MM, Dubey SK (2013) Lead resistant bacteria: lead resistance mechanisms, their applications in lead bioremediation and biomonitoring. Ecotoxicol Environ Saf 98:1–7
Naik MM, Khanolkar D, Dubey SK (2013) Lead-resistant Providencia alcalifaciens strain 2EA bioprecipitates Pb2+ as lead phosphate. Lett Appl Microbiol 56(2):99–104
Park JH, Bolan N, Megharaj M, Naidu R (2011) Concomitant rock phosphate dissolution and lead immobilization by phosphate solubilizing bacteria (Enterobacter sp.) J Environ Manag 92:1115–1120
Pérez JAM, Rafael García-Ribera R, Quesada T, Aguilera M, Ramos-Cormenzana A, Monteoliva-Sánchez M (2008) Biosorption of heavy metals by the exopolysaccharide produced by Paenibacillus jamilae. World J Microbiol Biotechnol 24(11):2699–2704
Rasulov BA, Yili A, Aisa HA (2013) Biosorption of metal ions by exopolysaccharide produced by Azotobacter chroococcum XU1. J Environ Prot 4:989–993
Ray L, Paul S, Bera D, Chattopadhyay P (2006) Bioaccumulation of Pb (II) from aqueous solutions by Bacillus cereus M1 16. Journal of Hazardous Substance Research 5(1):1–1
Rodríguez CE, Quesada A, Rodríguez E (2006) Nickel biosorption by Acinetobacter baumannii and Pseudomonas aeruginosa isolated from industrial wastewater. Braz J Microbiol 37(4):465–467
Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160:47–56
Sheng GP, Xu J, Li WH, Yu HQ (2013) Quantification of the interactions between Ca2+, Hg2+ and extracellular polymeric substances (EPS) of sludge. Chemosphere 93(7):1436–1441
Srivastava S, Thakur IS (2007) Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent. Biodegradation 18:637–646
Tong S, von Schirnding YE, Prapamontol T (2000) Environmental lead exposure: a public health problem of global dimensions. Bull World Health Organ 78:1068–1077
Valgas C, Souza SMD, Smânia EF, Smânia A Jr (2007) Screening methods to determine antibacterial activity of natural products. Braz J Microbiol 38(2):369–380
Watt G, Britton A, Gilmour HG, Moore MR, Murray GD, Robertson SJ (2000) Public health implications of new guidelines for lead in drinking water: a case study in an area with historically high water lead levels. Food Chem Toxicol 381:S73–S79
Yahya SK, Zakaria ZA, Samin J, Raj AS, Ahmad WA (2012) Isotherm kinetics of Cr(III) removal by non-viable cells of Acinetobacter haemolyticus. Colloids Surf B Biointerfaces 94:362–368
Zaidi S, Usmani S, Singh BR, Musarrat J (2006) Significance of Bacillus subtilis strain SJ-101 as a bioinoculant for concurrent plant growth promotion and nickel accumulation in Brassica juncea.Chemosphere. 64(6):991-7
Acknowledgements
The authors are grateful to TEQIP-II and MNNIT, Allahabad for financial support for this research. Ms. Anamika Kushwaha acknowledges TEQIP-II for their financial support in the form of fellowship. The authors are grateful to CMDR, MNNIT, Allahabad for providing facility for BIOLOG. The SEM and TEM imaging facilities provided by SAIF, AIIMS New Delhi are greatly acknowledged.
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Kushwaha, A., Rani, R., Kumar, S. et al. A new insight to adsorption and accumulation of high lead concentration by exopolymer and whole cells of lead-resistant bacterium Acinetobacter junii L. Pb1 isolated from coal mine dump. Environ Sci Pollut Res 24, 10652–10661 (2017). https://doi.org/10.1007/s11356-017-8752-8
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DOI: https://doi.org/10.1007/s11356-017-8752-8