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Biomass and lipid production from Nannochloropsis oculata growth in raceway ponds operated in sequential batch mode under greenhouse conditions

  • 4th International Symposium on Environmental Biotechnology and Engineering-2014
  • Published:
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Abstract

The effect of sequential batch cultures of the marine microalgae Nannochloropsis oculata on lipid and biomass production was studied in 200-L raceway ponds for 167 days (nine harvesting cycles) during winter and spring seasons under greenhouse conditions. The highest biomass concentration and productivity were 1.2 g/L and 49.8 mg/L/day on days 73 (5th cycle) and 167 (9th cycle), respectively. The overall interval of lipid production was between 131 and 530 mg/L. Despite the daily and seasonal variations of light irradiance (0–1099 μmol photon/m2 s), greenhouse temperature (2.1–50.7 °C), and culture temperature (12.5–31.4 °C), ANOVA analysis showed no statistical difference (p value > 0.01) on the fatty acid methyl ester (FAMES) composition over the nine harvesting cycles evaluated. The most abundant FAMES were palmitic (C16:0), stearic (C18:0) and palmitoleic (C16:1∆9) acids with 37.1, 28.6, and 8.4 %, respectively. The sequential batch cultures of N. oculata in raceway ponds showed an increasing biomass production in each new cycle while keeping the quality of the fatty acid mixture under daily and seasonal variations of light irradiance and temperature.

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Abbreviations

DCW:

Dry cell weight

FAMES:

Fatty acid methyl esters

SBC:

Sequential batch culture

References

  • Bao Y, Liu M, Wu X, Cong W, Ning Z (2012) In situ carbon supplementation in large-scale cultivations of Spirulina platensis in open raceway pond. Biotechnol Bioprocess Eng 17(1):93–99

    Article  CAS  Google Scholar 

  • Borowitzka M, Moheimani N (2013) Open pond culture systems. In: Borowitzka MA, Moheimani NR (eds) Algae for Biofuels and Energy, vol 5. Developments in Applied Phycology. Springer Netherlands, pp 133–152

  • Boruff BJ, Moheimani NR, Borowitzka MA (2015) Identifying locations for large-scale microalgae cultivation in Western Australia: a GIS approach. Appl Energy 149:379–391

    Article  Google Scholar 

  • Cabello J, Toledo-Cervantes A, Sánchez L, Revah S, Morales M (2015) Effect of the temperature, pH and irradiance on the photosynthetic activity by Scenedesmus obtusiusculus under nitrogen replete and deplete conditions. Bioresour Technol 181:128–135

    Article  CAS  Google Scholar 

  • Capellán-Pérez I, Mediavilla M, de Castro C, Carpintero Ó, Miguel LJ (2014) Fossil fuel depletion and socio-economic scenarios: an integrated approach. Energy 77:641–666

    Article  Google Scholar 

  • Chen M, Tang H, Ma H, Holland TC, Ng KY, Salley SO (2011) Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta. Bioresour Technol 102(2):1649–1655

    Article  CAS  Google Scholar 

  • Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25(3):294–306

    Article  CAS  Google Scholar 

  • Chiu SY, Kao CY, Tsai MT, Ong SC, Chen CH, Lin CS (2009) Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresour Technol 100(2):833–838

    Article  CAS  Google Scholar 

  • Converti A, Casazza AA, Ortiz EY, Perego P, Del Borghi M (2009) Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem Eng Process 48(6):1146–1151

    Article  CAS  Google Scholar 

  • Das P, Aziz SS, Obbard JP (2011) Two phase microalgae growth in the open system for enhanced lipid productivity. Renew Energy 36(9):2524–2528

    Article  CAS  Google Scholar 

  • Fábregas J, Patiño M, Arredondo-Vega BO, Tobar JL, Otero A (1995) Renewal rate and nutrient concentration as tools to modify productivity and biochemical composition of cyclostat cultures of the marine microalga Dunaliella tertiolecta. Appl Microbiol Biotechnol 44(3):287–292

    Article  Google Scholar 

  • García-Ferris C, de los Ríos A, Ascaso C, Moreno J (1996) Correlated biochemical and ultrastructural changes in nitrogen-starved Euglena glacilis. J Phycol 32(6):953–963

    Article  Google Scholar 

  • Gwak Y, Hwang Y, Wang B, Kim M, Jeong J, Lee C, Hu Q, Han D, Jin E (2014) Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis. J Exp Bot 65(15):4317–4334

    Article  CAS  Google Scholar 

  • Hase R, Oikawa H, Sasao C, Morita M, Watanabe Y (2000) Photosynthetic production of microalgal biomass in a raceway system under greenhouse conditions in Sendai city. J Biosci Bioeng 89(2):157–163

    Article  CAS  Google Scholar 

  • He Q, Yang H, Wu L, Hu C (2015) Effect of light intensity on physiological changes, carbon allocation and neutral lipid accumulation in oleaginous microalgae. Bioresour Technol 191:219–228

    Article  CAS  Google Scholar 

  • Ho SH, Lu WB, Chang JS (2012) Photobioreactor strategies for improving the CO2 fixation efficiency of indigenous Scenedesmus obliquus CNW-N: statistical optimization of CO2 feeding, illumination, and operation mode. Bioresour Technol 105:106–113

    Article  CAS  Google Scholar 

  • Ho SH, Chen CNN, Lai YY, Lu WB, Chang JS (2014) Exploring the high lipid production potential of a thermotolerant microalga using statistical optimization and semi-continuous cultivation. Bioresour Technol 163:128–135

    Article  CAS  Google Scholar 

  • Janssen M, Bresser L, Baijens T, Tramper J, Mur LR, Snel JFH, Wijffels RH (2000) Scale-up aspects of photobioreactors: effects of mixing-induced light/dark cycles. J Appl Phycol 12:225–237

    Article  CAS  Google Scholar 

  • Keeney DR, Nelson DW (1982) Nitrogen inorganic forms. In: Black CA (ed) Methods of soil analysis, part 2: agronomy. American Society of Agronomy, Madison, pp 643–698

    Google Scholar 

  • Kliphuis AM, Janssen M, van den End EJ, Martens DE, Wijffels RH (2011) Light respiration in Chlorella sorokiniana. J Appl Phycol 23:935–947

    Article  CAS  Google Scholar 

  • Liu J, Huang J, Sun Z, Zhong Y, Jiang Y, Chen F (2011) Differential lipid and fatty acid profiles of photoautotrophic and heterotrophic Chlorella zofingiensis: assessment of algal oils for biodiesel production. Bioresour Technol 102(1):106–110

    Article  CAS  Google Scholar 

  • Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: a review. Renew Sust Energ Rev 14(1):217–232

    Article  CAS  Google Scholar 

  • Merchuk JC, Ronen M, Giris S, Arad SM (1998) Light/dark cycles in the growth of the red microalga Porphyridium sp. Biotechnol Bioeng 59:705–713

  • Moheimani N, Borowitzka M (2006) The long-term culture of the coccolithophore Pleurochrysis carterae (Haptophyta) in outdoor raceway ponds. J Appl Phycol 18(6):703–712

    Article  Google Scholar 

  • Morais MG, Radmann EM, Andrade MR, Teixeira GG, Brusch LRF, Costa JAV (2009) Pilot scale semicontinuous production of Spirulina biomass in southern Brazil. Aquaculture 294:60–64

    Article  Google Scholar 

  • Moreno J, Vargas MÁ, Rodrıguez H, Rivas J, Guerrero MG (2003) Outdoor cultivation of a nitrogen-fixing marine cyanobacterium, Anabaena sp. ATCC 33047. Biomol Eng 20:191–197

    Article  CAS  Google Scholar 

  • Pawlowski A, Mendoza JL, Guzmán JL, Berenguel M, Acién FG, Dormido S (2015) Selective pH and dissolved oxygen control strategy for a raceway reactor within an event-based approach. Control Eng Pract 44:209–218

    Article  Google Scholar 

  • Radmann EM, Reinehr CO, Costa JAV (2007) Optimization of the repeated batch cultivation of microalga Spirulina platensis in open raceway ponds. Aquaculture 265:118–126

    Article  Google Scholar 

  • Raes EJ, Isdepsky A, Muylaert K, Borowitzka MA, Moheimani NR (2014) Comparison of growth of Tetraselmis in a tubular photobioreactor (Biocoil) and a raceway pond. J Appl Phycol 26(1):247–255

    Article  CAS  Google Scholar 

  • Richardson JW, Johnson MD, Outlaw JL (2012) Economic comparison of open pond raceways to photo bio-reactors for profitable production of algae for transportation fuels in the Southwest. Algal Res 1(1):93–100

    Article  Google Scholar 

  • Ritchie RJ (2006) Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynth Res 89(1):27–41

    Article  CAS  Google Scholar 

  • Rodolfi L, Chini Zittelli G, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102(1):100–112

    Article  CAS  Google Scholar 

  • Selinummi J, Seppala J, Yli-Harja O, Puhakka JA (2005) Software for quantification of labeled bacteria from digital microscope images by automated image analysis. BioTechniques 39(6):859–863

    Article  CAS  Google Scholar 

  • Silva Benavides AM, Torzillo G, Kopecký J, Masojídek J (2013) Productivity and biochemical composition of Phaeodactylum tricornutum (Bacillariophyceae) cultures grown outdoors in tubular photobioreactors and open ponds. Biomass Bioenergy 54:115–122

    Article  CAS  Google Scholar 

  • Singh J, Gu S (2010) Commercialization potential of microalgae for biofuels production. Renew Sust Energ Rev 14(9):2596–2610

    Article  CAS  Google Scholar 

  • Solovchenko AE, Khozin-Goldberg I, Cohen Z, Merzlyak MN (2009) Carotenoid-to-chlorophyll ratio as a proxy for assay of total fatty acids and arachidonic acid content in the green microalga Parietochloris incisa. J Appl Phycol 21(3):361–366

    Article  CAS  Google Scholar 

  • Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Optimization of Nannochloropsis oculata growth using the response surface method. J Chem Technol Biotechnol 81(6):1049–1056

    Article  CAS  Google Scholar 

  • Strickland JDH, Parsons TR (1972) A practical handbook of seawater analysis vol 1977, vol no. 167; no. 1977. Fisheries Research Board of Canada, Ottawa

    Google Scholar 

  • Suh SS, Kim SJ, Hwang J, Park M, Lee TK, Eui-Joon Kil EJ, Lee L (2015) Fatty acid methyl ester profiles and nutritive values of 20 marine microalgae in Korea. Asian Pac J Trop Med 8(3):191–196

    Article  CAS  Google Scholar 

  • Toledo-Cervantes A, Morales M, Novelo E, Revah S (2013) Carbon dioxide fixation and lipid storage by Scenedesmus obtusiusculus. Bioresour Technol 130:652–658

    Article  CAS  Google Scholar 

  • Zhou W et al (2012) Growing wastewater-born microalga Auxenochlorella protothecoides UMN280 on concentrated municipal wastewater for simultaneous nutrient removal and energy feedstock production. Appl Energy 98:433–440

    Article  CAS  Google Scholar 

  • Zhu CJ, Lee YK (1997) Determination of biomass dry weight of marine microalgae. J Appl Phycol 9(2):189–194

    Article  Google Scholar 

Download references

Acknowledgments

The authors are grateful for the financial support provided by the National Science and Technology Council (CONACYT) of Mexico, the Secretary for Research and Postgraduated Studies—Instituto Politécnico Nacional (SIP-IPN, grants 20130388 and 20144620), and the collaboration of Research and Development Center of CARSO. The authors would also like to thank Claudia Guerrero Barajas and Hervé Marie for their participation in reviewing the paper.

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Authors and Affiliations

  1. Departamento de Bioprocesos, Unidad Profesional Interdisciplinaria de Biotecnología - Instituto Politécnico Nacional (UPIBI - IPN), Av. Acueducto s/n Col. Barrio la Laguna Ticomán, 07340, Mexico City, Mexico

    Aarón Millán-Oropeza & Luis Fernández-Linares

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  1. Aarón Millán-Oropeza
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  2. Luis Fernández-Linares
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Correspondence to Luis Fernández-Linares.

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Millán-Oropeza, A., Fernández-Linares, L. Biomass and lipid production from Nannochloropsis oculata growth in raceway ponds operated in sequential batch mode under greenhouse conditions. Environ Sci Pollut Res 24, 25618–25626 (2017). https://doi.org/10.1007/s11356-016-7013-6

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  • DOI: https://doi.org/10.1007/s11356-016-7013-6

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