Skip to main content
SpringerLink
Log in

Static magnetic field exposure–induced oxidative response and caspase-independent apoptosis in rat liver: effect of selenium and vitamin E supplementations

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

In the present study, we investigated the implication of oxidative stress and apoptosis under static magnetic field (SMF) in the brain and liver. Moreover, we estimated the protective role of selenium and vitamin E in rat tissues against disorders induced by SMF. Exposure of rats to SMF (128 mT, 1 h/day during five consecutive days) increased the activity of catalase (CAT) (+24 %) in the liver but not in the brain. By contrast, the same treatment failed to alter malondialdehyde (MDA) concentration in the brain and liver. Exposure to SMF also induced hepatocyte apoptosis through a caspase-independent pathway involving mitochondrial apoptosis-inducing factor (AIF) but not in the brain. Selenium and vitamin E supplementations to SMF-exposed rats restored liver CAT activity but failed to minimize liver apoptosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Abdelmelek H, Molnar A, Servais S, Cottet-Emard JM, Pequignot JM, Favier R, Sakly M (2006) Skeletal muscle HSP72 and norepinephrine response to static magnetic field in rat. J Neural Transm 113:821–827

    Article  CAS  Google Scholar 

  • Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121

    Article  CAS  Google Scholar 

  • Amara S, Adbelmelek H, Mohsen S (2004) Effects of acute exposure to magnetic field on ionic composition of frog sciatic nerve. Pak J Med Sci 20:91–96

    Google Scholar 

  • Amara S, Abdelmelek H, Garrel C, Guiraud P, Douki T, Ravanat JL, Favier A, Sakly M, Ben Rhouma K (2007) Zinc supplementation ameliorates static magnetic field-induced oxidative stress in rat tissues. Environ Toxicol Pharmacol 23:193–197

    Article  CAS  Google Scholar 

  • Amara S, Douki T, Garel C, Favier A, Sakly M, Rhouma KB, Abdelmelek H (2009) Effects of static magnetic field exposure on antioxidative enzymes activity and DNA in rat brain. Gen Physiol Biophys 28:260–265

    Article  CAS  Google Scholar 

  • Brydon L, Petit L, Delagrange P, Strosberg AD, Jockers R (2001) Functional expression of MT2 (Mel1b) melatonin receptors in human PAZ6 adipocytes. Endocrinology 142:4264–4271

    Article  CAS  Google Scholar 

  • Chater S, Abdelmelek H, Couton D, Joulin V, Sakly M, Ben Rhouma K (2004) Effects of sub-acute exposure to magnetic field on synthesis of plasma corticosterone and liver metallothionein levels in female rats. Pak J Med Sci 20:219–223

    Google Scholar 

  • Chater S, Abdelmelek H, Couton D, Joulin V, Sakly M, Ben Rhouma K (2005) Sub-acute exposure to magnetic field induced apoptosis in thymus female rats. Pak J Med Sci 21:292–297

    Google Scholar 

  • Cregan SP, Fortin A, MacLaurin JG, Callaghan SM, Cecconi F, Yu SW, Dawson TM, Dawson VL, Park DS, Kroemer G, Slack RS (2002) Apoptosis-inducing factor is involved in the regulation of caspase-independent neuronal cell death. J Cell Biol 158:507–517

    Article  CAS  Google Scholar 

  • Dini L, Dwikat M, Panzarini E, Vergallo C, Tenuzzo B (2009) Morphofunctional study of 12-O-tetradecanoyl-13-phorbol acetate (TPA)-induced differentiation of U937 cells under exposure to a 6 mT static magnetic field. Bioelectromagnetics 30:352–364

    Article  CAS  Google Scholar 

  • Ghodbane S, Amara S, Arnaud J, Garrel C, Faure H, Favier A, Sakly M, Abdelmelek H (2011a) Effect of selenium pre-treatment on plasma antioxidant vitamins A (retinol) and E (alpha-tocopherol) in static magnetic field-exposed rats. Toxicol Ind Health 27:949–955

    Article  CAS  Google Scholar 

  • Ghodbane S, Amara S, Garrel C, Arnaud J, Ducros V, Favier A, Sakly M, Abdelmelek H (2011b) Selenium supplementation ameliorates static magnetic field-induced disorders in antioxidant status in rat tissues. Environ Toxicol Pharmacol 31:100–106

    Article  CAS  Google Scholar 

  • Goodman R, Blank M (2002) Insights into electromagnetic interaction mechanisms. J Cell Physiol 192:16–22

    Article  CAS  Google Scholar 

  • Jajte J, Grzegorczyk J, Zmyslony M, Rajkowska E (2002) Effect of 7 mT static magnetic field and iron ions on rat lymphocytes: apoptosis, necrosis and free radical processes. Bioelectrochemistry 57:107–111

    Article  CAS  Google Scholar 

  • Joubert V, Bourthoumieu S, Leveque P, Yardin C (2008) Apoptosis is induced by radiofrequency fields through the caspase-independent mitochondrial pathway in cortical neurons. Radiat Res 169:38–45

    Article  CAS  Google Scholar 

  • Kula B, Sobczak A, Kuska R (2000) Effects of static and ELF magnetic fields on free-radical processes in rat liver and kidney. Electro Magnetobiol 19:99–105

    Article  CAS  Google Scholar 

  • Loeffler M, Kroemer G (2000) The mitochondrion in cell death control: certainties and incognita. Exp Cell Res 256:19–26

    Article  CAS  Google Scholar 

  • Martinho A, Goncalves I, Santos CR (2013) Glucocorticoids regulate metallothionein-1/2 expression in rat choroid plexus: effects on apoptosis. Mol Cell Biochem 376:41–51

    Article  CAS  Google Scholar 

  • Mates JM, Sanchez-Jimenez FM (2000) Role of reactive oxygen species in apoptosis: implications for cancer therapy. Int J Biochem Cell Biol 32:157–170

    Article  CAS  Google Scholar 

  • Mathiasen IS, Jaattela M (2002) Triggering caspase-independent cell death to combat cancer. Trends Mol Med 8:212–220

    Article  CAS  Google Scholar 

  • Miyakoshi J (2005) Effects of static magnetic fields at the cellular level. Prog Biophys Mol Biol 87:213–223

    Article  CAS  Google Scholar 

  • Okano H (2008) Effects of static magnetic fields in biology: role of free radicals. Front Biosci 13:6106–6125

    Article  CAS  Google Scholar 

  • Placer ZA, Cushman LL, Johnson BC (1966) Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem 16:359–364

    Article  CAS  Google Scholar 

  • Politanski P, Rajkowska E, Pawlaczyk-Luszczynska M, Dudarewicz A, Wiktorek-Smagur A, Sliwinska-Kowalska M, Zmyslony M (2010) Static magnetic field affects oxidative stress in mouse cochlea. Int J Occup Med Environ Health 23:377–384

    Article  Google Scholar 

  • Potenza L, Martinelli C, Polidori E, Zeppa S, Calcabrini C, Stocchi L, Sestili P, Stocchi V (2010) Effects of a 300 mT static magnetic field on human umbilical vein endothelial cells. Bioelectromagnetics 31:630–639

    Article  CAS  Google Scholar 

  • Shin SJ (2003) High levels of apoptosis induced by total body irradiation in mice fed a low protein-low vitamin E diet. Food Chem Toxicol 41:665–670

    Article  CAS  Google Scholar 

  • Susin SA, Zamzami N, Castedo M, Hirsch T, Marchetti P, Macho A, Daugas E, Geuskens M, Kroemer G (1996) Bcl-2 inhibits the mitochondrial release of an apoptogenic protease. J Exp Med 184:1331–1341

    Article  CAS  Google Scholar 

  • Tenuzzo B, Vergallo C, Dini L (2009) Effect of 6mT static magnetic field on the bcl-2, bax, p53 and hsp70 expression in freshly isolated and in vitro aged human lymphocytes. Tissue Cell 41:169–179

    Article  CAS  Google Scholar 

  • Zhadin MN (2001) Review of Russian literature on biological action of DC and low-frequency AC magnetic fields. Bioelectromagnetics 22:27–45

    Article  CAS  Google Scholar 

  • Zmyślony M, Jajte J, Rajkowska E, Szmigielski S (1998) Weak (5 mT) static magnetic field stimulates lipid peroxidation in isolated rat liver microsomes in vitro. Electro Magnetobiol 17:109–113

    Google Scholar 

Download references

Conflict of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.

Author information

Authors and Affiliations

  1. Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, Université de Carthage, 7021, Jarzouna, Tunisia

    Soumaya Ghodbane, Mohamed Ammari, Aida Lahbib, Mohsen Sakly & Hafedh Abdelmelek

  2. Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis el Manar, Tunis, Tunisia

    Mohamed Ammari

Authors
  1. Soumaya Ghodbane
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mohamed Ammari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aida Lahbib
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohsen Sakly
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hafedh Abdelmelek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Soumaya Ghodbane.

Additional information

Responsible editor: Philippe Garrigues

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ghodbane, S., Ammari, M., Lahbib, A. et al. Static magnetic field exposure–induced oxidative response and caspase-independent apoptosis in rat liver: effect of selenium and vitamin E supplementations. Environ Sci Pollut Res 22, 16060–16066 (2015). https://doi.org/10.1007/s11356-015-4802-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-015-4802-2

Keywords

Search

Navigation