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Effect of physical sediments reworking on hydrocarbon degradation and bacterial community structure in marine coastal sediments

  • DECAPAGE Project: Hydrocarbon degradation in coastal sediments*
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Abstract

The present study aimed to examine whether the physical reworking of sediments by harrowing would be suitable for favouring the hydrocarbon degradation in coastal marine sediments. Mudflat sediments were maintained in mesocosms under conditions as closer as possible to those prevailing in natural environments with tidal cycles. Sediments were contaminated with Ural blend crude oil, and in half of them, harrowing treatment was applied in order to mimic physical reworking of surface sediments. Hydrocarbon distribution within the sediment and its removal was followed during 286 days. The harrowing treatment allowed hydrocarbon compounds to penetrate the first 6 cm of the sediments, and biodegradation indexes (such as n-C18/phytane) indicated that biodegradation started 90 days before that observed in untreated control mesocosms. However, the harrowing treatment had a severe impact on benthic organisms reducing drastically the macrofaunal abundance and diversity. In the harrowing-treated mesocosms, the bacterial abundance, determined by 16S rRNA gene Q-PCR, was slightly increased; and terminal restriction fragment length polymorphism (T-RFLP) analyses of 16S rRNA genes showed distinct and specific bacterial community structure. Co-occurrence network and canonical correspondence analyses (CCA) based on T-RFLP data indicated the main correlations between bacterial operational taxonomic units (OTUs) as well as the associations between OTUs and hydrocarbon compound contents further supported by clustered correlation (ClusCor) analysis. The analyses highlighted the OTUs constituting the network structural bases involved in hydrocarbon degradation. Negative correlations indicated the possible shifts in bacterial communities that occurred during the ecological succession.

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Acknowledgments

We acknowledge the support of the French programme ANR DECAPAGE (project ANR-CESA-2011-006 01). We would like to thank all partners of the DECAPAGE project and MELODY group for their useful discussions. We acknowledge the Regional Platform for Environmental Microbiology PREMICE supported by the Aquitaine Regional Government Council (France) and the urban community of Pau-Pyrénées (France). We thank the Direction Générale de l’Armement (Edgar) and the Aquitaine Regional Government Council (France) for financial support to JA. We acknowledge B. Bouyssiere, S. Guasco, M.S. Goñi-Urriza and J. Gury for technical assistance during sampling campaign.

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Authors and Affiliations

  1. Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l’Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France

    Robert Duran, Claire Gassie, Fanny Terrisse, Justine Abella, Christine Cagnon & Cristiana Cravo-Laureau

  2. Aix Marseille Université, CNRS, IRD, MIO UM 110, 13288, Marseille, France

    Patricia Bonin, Cecile Militon, Valérie Michotey & Philippe Cuny

  3. Centre de Documentation, de Recherche et d’Expérimentations sur les pollutions accidentelles des Eaux, 715 rue Alain Colas, CS 41836, Brest, France

    Ronan Jezequel & Karine Dubosc

  4. Université de Toulouse; INP, UPS; EcoLab (Laboratoire écologie fonctionnelle et environnement), 118 Route de Narbonne, 31062, Toulouse, France

    Franck Gilbert

  5. CNRS; EcoLab, 31062, Toulouse, France

    Franck Gilbert

  6. Université de Pau et des Pays de l’Adour, Bâtiment IBEAS, BP 1155, 64013, Pau Cedex, France

    Robert Duran

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  1. Robert Duran
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  2. Patricia Bonin
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  3. Ronan Jezequel
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  4. Karine Dubosc
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  8. Christine Cagnon
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  11. Franck Gilbert
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  13. Cristiana Cravo-Laureau
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Correspondence to Robert Duran.

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Duran, R., Bonin, P., Jezequel, R. et al. Effect of physical sediments reworking on hydrocarbon degradation and bacterial community structure in marine coastal sediments. Environ Sci Pollut Res 22, 15248–15259 (2015). https://doi.org/10.1007/s11356-015-4373-2

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