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5-Aminolevulinic acid ameliorates cadmium-induced morphological, biochemical, and ultrastructural changes in seedlings of oilseed rape

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Abstract

Due to its prolific growth, oilseed rape (Brassica napus L.) can be grown successfully for phytoremediation of cadmium (Cd)-contaminated soils. Nowadays, use of plant growth regulators against heavy metals stress is one of the major objectives of researchers. The present study evaluates the ameliorate effects of 5-aminolevulinic acid (ALA, 0, 0.4, 2, and 10 mg/l) on the growth of oilseed rape (B. napus L. cv. ZS 758) seedlings under Cd stress (0, 100, and 500 μM). Results have shown that Cd stress hampered the seedling growth by decreasing the radical and hypocotyls length, shoot and root biomass, chlorophyll content, and antioxidants enzymes. On the other hand, Cd stress increased the level of malondialdehyde (MDA) and production of H2O2 and accumulation of Cd in the shoots. The microscopic study of leaf mesophyll cells showed that toxicity of Cd totally destroyed the whole cell structure, and accumulation of Cd also appeared in micrographs. Application of ALA at lower dosage (2 mg/l) enhanced the seedling growth and biomass. The results showed that 2 mg/l ALA significantly improved chlorophyll content under Cd stress and decreased the level of Cd contents in shoots. Application of ALA reduced the MDA and H2O2 levels in the cotyledons. The antioxidants enzymes (ascorbate peroxidase, peroxidase, catalase, glutathione reductase, and superoxide dismutase) enhanced their activities significantly with the application of 2 mg/l ALA under Cd stress. This study also indicated that higher dosage of ALA (10 mg/l) imposed the negative effect on the growth of oilseed rape. Microscopic study showed that application of ALA alleviated the toxic effects of Cd in the mesophyll cell and improved the cell structure. Use of 2 mg/l ALA under 500 μM Cd was found to be more effective, and under this dosage, cell structure was clear, with obvious cell wall and cell membrane as well as a big nucleus, which was found with well-developed two or more nucleoli. Chloroplast was almost round in shape and contained thylakoids membranes and grana, but starch grains were not found in chloroplast comparatively to other treatments. On the basis of our results, we can conclude that ALA has a promotive effect which could improve plant survival under Cd stress.

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Acknowledgements

This work was supported by the National High Technology Research and Development Program of China (2011AA10A206), the National Key Science & Technology Supporting Program of China (2010BAD01B01, 2010BAD01B04), the National Natural Science Foundation of China (31000678, 31071698), and the Science and Technology Department of Zhejiang Province (2012C12902-1, 2011R50026-5).

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Authors and Affiliations

  1. Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China

    Basharat Ali, C. R. Huang, M. K. Daud, X. X. Geng, H. B. Liu & W. J. Zhou

  2. Agricultural Experiment Station, Zhejiang University, Hangzhou, 310058, China

    Z. Y. Qi & W. J. Zhou

  3. Department of Environmental Sciences, Government College University, Faisalabad, 38000, Pakistan

    Shafaqat Ali

  4. Department of Biotechnology & Genetic Engineering, Kohat University of Science & Technology, Kohat, 26000, Pakistan

    M. K. Daud

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  1. Basharat Ali
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  2. C. R. Huang
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Correspondence to W. J. Zhou.

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Communicated by Elena Maestri

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Ali, B., Huang, C.R., Qi, Z.Y. et al. 5-Aminolevulinic acid ameliorates cadmium-induced morphological, biochemical, and ultrastructural changes in seedlings of oilseed rape. Environ Sci Pollut Res 20, 7256–7267 (2013). https://doi.org/10.1007/s11356-013-1735-5

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