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Accumulation, biotransformation, and biochemical responses after exposure to arsenite and arsenate in the estuarine polychaete Laeonereis acuta (Nereididae)

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Abstract

Introduction

This study aimed to analyze antioxidant responses and oxidative damage induced by two inorganic forms of arsenic (As; AsIII and AsV) in an estuarine polychaete species, Laeonereis acuta (Nereididae). The capacity of arsenic biotransformation was also evaluated through the methylation process considering the activity of a key enzyme involved in the metabolization process.

Materials and methods

Worms were exposed to 50 μg (AsIII or AsV)/l during 2 or 7 days, plus a control group. Endpoints analyzed included concentration of reactive oxygen species (ROS), activities of antioxidant enzymes such as glutathione reductase (GR), total glutathione-S-transferase (GST), and omega isoform (GST Ω), glucose-6-phosphate deshydrogenase (G6PDH), levels of the antioxidant glutathione (GSH), and lipid peroxides concentration (TBARS).

Results and discussion

Results showed: (1) GR inhibition after 2-day exposure to both As forms (p < 0.05); (2) GST Ω inhibition after 7-day exposure to AsIII paralleled by an increase in total GST activity (p < 0.05); (3) augmented G6PDH activity after 7-day exposure to both As forms (p < 0.05); (4) no differences in terms of ROS and TBARS; and (5) inhibition of GST Ω activity in AsIII exposed worms, which was concomitant with a lowering of mono- and dymethylated arsenic species.

Conclusion

These results confirm the reactivity of some biochemical variables of L. acuta to As and indicates its importance as a sentinel species in estuarine regions with presence of arsenic.

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Grants, sponsors, and funding sources

The authors wish to thank the Brazilian Agency CNPq that funded a short-time fellowship to J. Ventura-Lima to stay in Dr. Regoli lab. (process 200603/2008-6, SWE program). J.M. Monserrat is a research fellow funded by CNPq (process 304917/2006-0). J. Ventura-Lima is a graduate fellow from the Brazilian Agency CAPES. J. Ventura-Lima acknowledges the post-doctoral fellow from Programa Ciências do Mar - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brasília, DF, Brasil.

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Authors and Affiliations

  1. Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande—FURG, Cx. P. 474, CEP 96.201-900, Rio Grande, RS, Brazil

    Juliane Ventura-Lima, Patrícia Baptista Ramos & José M. Monserrat

  2. Programa de Pós-Graduação em Ciências Fisiológicas—Fisiologia Animal Comparada, Universidade Federal do Rio Grande—FURG, Rio Grande, RS, Brazil

    Juliane Ventura-Lima & José M. Monserrat

  3. Istituto di Biologia e Genetica, Università Politecnica delle Marche, 60100, Ancona, Italy

    Daniele Fattorini & Francesco Regoli

  4. Departamento de Química, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil

    Luis Ferraz & Leandro Machado de Carvalho

Authors
  1. Juliane Ventura-Lima
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  2. Patrícia Baptista Ramos
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  3. Daniele Fattorini
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  4. Francesco Regoli
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  5. Luis Ferraz
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  6. Leandro Machado de Carvalho
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  7. José M. Monserrat
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Corresponding author

Correspondence to José M. Monserrat.

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Responsible editor: Vera Slaveykova

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Ventura-Lima, J., Ramos, P.B., Fattorini, D. et al. Accumulation, biotransformation, and biochemical responses after exposure to arsenite and arsenate in the estuarine polychaete Laeonereis acuta (Nereididae). Environ Sci Pollut Res 18, 1270–1278 (2011). https://doi.org/10.1007/s11356-011-0478-4

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  • DOI: https://doi.org/10.1007/s11356-011-0478-4

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