Skip to main content

Advertisement

SpringerLink
Log in

A basic in vitro study on effective conservative combined therapy for malignant tumors

  • Original Article
  • Published:
Oral Radiology Aims and scope Submit manuscript

Abstract

Objectives

The study aimed to clarify the antitumor effects of radiotherapy, chemotherapy, hyperthermia, and combinations thereof in an effort to devise a highly effective noninvasive regimen.

Methods

The human KB cell line, derived from an epidermal carcinoma of the mouth, was used for the experiments. The antitumor effects of monotherapy—with radiotherapy of 6-MV X-rays, 43°C hyperthermia, and chemotherapy with the taxoid antineoplastic drug docetaxel—as well as combinations of these therapies were evaluated using a colony assay.

Results

Compared with monotherapy of 6-MV X-rays, 43°C heat, and docetaxel, radiation combined with 43°C heat showed a slight increase in sensitization; radiation or 43°C heat combined with docetaxel showed stronger sensitization. Furthermore, more pronounced sensitization was observed with a three-way combination of radiation, 43°C heat, and docetaxel.

Conclusions

These results suggest that the combined use of radiation, 43°C heat, and docetaxel will improve the therapeutic efficacy against malignant tumors by increasing sensitization to the antitumor effects of each therapy. The results also suggest that it is possible to reduce the dosage of the individual therapies in combination therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Rao W, Deng ZS, Liu J. A review of hyperthermia combined with radiotherapy/chemotherapy on malignant tumors. Crit Rev Biomed Eng. 2010;38:101–16.

    PubMed  Google Scholar 

  2. Seagren SL. Recent advances in radiation therapy of head and neck cancer. Head Neck Surg. 1982;4:227–32.

    Article  PubMed  Google Scholar 

  3. Maehara Y, Kuwano H, Kitamura K, Matsuda H, Sugimachi K. Hyperthermochemoradiotherapy for esophageal cancer. Anticancer Res. 1992;12:805–10.

    PubMed  Google Scholar 

  4. Yonemura Y, Hashimoto T, Miyata R, Takegawa S, Kamata T, Katayama K, et al. A case of gastric cancer successfully treated with hyperthermo-chemo-radiotherapy. Gan To Kagaku Ryoho. 1985;12:1491–5. (in Japanese).

    PubMed  Google Scholar 

  5. Day FL, Leong T, Ngan S, Thomas R, Jefford M, Zalcberg JR, et al. Phase I trial of docetaxel, cisplatin and concurrent radical radiotherapy in locally advanced oesophageal cancer. Br J Cancer. 2011;104:265–71.

    Article  PubMed  Google Scholar 

  6. Dillman RO. Cancer immunotherapy. Cancer Biother Radiopharm. 2011;26:1–64.

    Article  PubMed  Google Scholar 

  7. Kolev M, Towner L, Donev R. Complement in cancer and cancer immunotherapy. Arch Immunol Ther Exp. 2011;59:407–19.

    Article  Google Scholar 

  8. Cáceres W, Cruz-Chacón A. Renal cell carcinoma: molecularly targeted therapy. P R Health Sci J. 2011;30:73–7.

    PubMed  Google Scholar 

  9. Goerner M, Seiwert TY, Sudhoff H. Molecular targeted therapies in head and neck cancer—an update of recent developments. Head Neck Oncol. 2010;2:8.

    Article  PubMed  Google Scholar 

  10. Vaupel PW. The influence of tumor blood flow and microenvironmental factors on the efficacy of radiation, drugs and localized hyperthermia. Klin Pädiatr. 1997;209:243–9.

    Article  PubMed  Google Scholar 

  11. Chaplin DJ. The effect of therapy on tumour vascular function. Int J Radiat Biol. 1991;60:311–25.

    Article  PubMed  Google Scholar 

  12. Thrall DE. Biologic basis of radiation therapy. Vet Clin North Am Small Anim Pract. 1997;27:21–35.

    PubMed  Google Scholar 

  13. Hildebrandt B, Wust P, Ahlers O, Dieing A, Sreenivasa G, Kerner T, et al. The cellular and molecular basis of hyperthermia. Crit Rev Oncol Hematol. 2002;43:33–56.

    Article  PubMed  Google Scholar 

  14. Miller ML, Ojima I. Chemistry and chemical biology of taxane anticancer agents. Chem Rec. 2001;1:195–211.

    Article  PubMed  Google Scholar 

  15. Ganansia-Leymarie V, Bischoff P, Bergerat JP, Holl V. Signal transduction pathways of taxanes-induced apoptosis. Curr Med Chem Anticancer Agents. 2003;3:291–306.

    Article  PubMed  Google Scholar 

  16. Hernández-Vargas H, Palacios J, Moreno-Bueno G. Telling cells how to die: docetaxel therapy in cancer cell lines. Cell Cycle. 2007;6:780–3.

    Article  PubMed  Google Scholar 

  17. Sanofi-Aventis. Total product information summary. Paris: Sanofi-Aventis; 2006. p. 86 (in Japanese)

  18. Rose PG. Chemoradiotherapy for cervical cancer. Eur J Cancer. 2002;38:270–8.

    Article  PubMed  Google Scholar 

  19. Dahl O, Mella O. Referee: hyperthermia alone or combined with cisplatin in addition to radiotherapy for advanced uterine cervical cancer. Int J Hyperth. 2002;18:25–30.

    Article  Google Scholar 

  20. Blasiak J, Widera K, Pertyński T. Hyperthermia can differentially modulate the repair of doxorubicin-damaged DNA in normal and cancer cells. Acta Biochim Pol. 2003;50:191–5.

    PubMed  Google Scholar 

  21. Falk MH, Issels RD. Hyperthermia in oncology. Int J Hyperth. 2001;17:1–18.

    Article  Google Scholar 

  22. Liang H, Zhan HJ, Wang BG, Pan Y, Hao XS. Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice. World J Gastroenterol. 2007;13:4365–71.

    PubMed  Google Scholar 

  23. Wust P, Hildebrandt B, Sreenivasa G, Rau B, Gellermann J, Riess H, et al. Hyperthermia in combined treatment of cancer. Lancet Oncol. 2002;3:487–97.

    Article  PubMed  Google Scholar 

  24. Sanofi-Aventis. Total product information summary. Paris: Sanofi-Aventis; 2006. p. 85 (in Japanese)

  25. Sanofi-Aventis. Total product information summary. Paris: Sanofi-Aventis; 2006. p. 88 (in Japanese)

  26. Owen MR, Alarcón T, Maini PK, Byrne HM. Angiogenesis and vascular remodelling in normal and cancerous tissues. J Math Biol. 2009;58:689–721.

    Article  PubMed  Google Scholar 

  27. Li J, Huang S, Armstrong EA, Fowler JF, Harari PM. Angiogenesis and radiation response modulation after vascular endothelial growth factor receptor-2 (VEGFR2) blockade. Int J Radiat Oncol Biol Phys. 2005;62:1477–85.

    Article  PubMed  Google Scholar 

  28. Song CW. Effect of local hyperthermia on blood flow and microenvironment: a review. Cancer Res. 1984;44(10 Suppl):4721s–30s.

    PubMed  Google Scholar 

  29. Wouters BG, Weppler SA, Koritzinsky M, Landuyt W, Nuyts S, Theys J, et al. Hypoxia as a target for combined modality treatments. Eur J Cancer. 2002;38:240–57.

    Article  PubMed  Google Scholar 

  30. Sonveaux P. Provascular strategy: targeting functional adaptations of mature blood vessels in tumors to selectively influence the tumor vascular reactivity and improve cancer treatment. Radiother Oncol. 2008;86:300–13.

    Article  PubMed  Google Scholar 

  31. Sivridis E, Giatromanolaki A, Anastasiadis P, Georgiou L, Gatter KC, Harris AL, et al. Angiogenic co-operation of VEGF and stromal cell TP in endometrial carcinomas. J Pathol. 2002;196:416–22.

    Article  PubMed  Google Scholar 

  32. Giatromanolaki A, Sivridis E, Koukourakis MI. Tumour angiogenesis: vascular growth and survival. APMIS. 2004;112:431–40.

    Article  PubMed  Google Scholar 

  33. Giatromanolaki A, Koukourakis MI, Stathopoulos GP, Kapsoritakis A, Paspatis G, Kakolyris S, et al. Angiogenic interactions of vascular endothelial growth factor, of thymidine phosphorylase, and of p53 protein expression in locally advanced gastric cancer. Oncol Res. 2000;12:33–41.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Radiology, Department of Maxillofacial Diagnostic Science, Kanagawa Dental College, 82 Inaoka, Yokosuka, Kanagawa, 238-8580, Japan

    Takashi Sakurai, Masahiro Hara, Ryota Kawamata, Yusuke Kozai & Hisashi Innami

Authors
  1. Takashi Sakurai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masahiro Hara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ryota Kawamata
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yusuke Kozai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hisashi Innami
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takashi Sakurai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sakurai, T., Hara, M., Kawamata, R. et al. A basic in vitro study on effective conservative combined therapy for malignant tumors. Oral Radiol 28, 48–54 (2012). https://doi.org/10.1007/s11282-012-0081-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11282-012-0081-5

Keywords

Search

Navigation