Abstract
Chernevaya taiga of Western Siberia, Russia, is a unique ecosystem characterized by fertile soil, exceptionally large herbaceous plant sizes, and extraordinarily rapid rates of plant residue degradation. We expected that growing crops on soil collected from Chernevaya taiga, which has never been used for agricultural purposes before, would result in a distinct rhizospheric fungal community. This community could potentially yield novel, potent biostimulators and biocontrol fungi for modern agriculture. To check this idea, we used high-throughput ITS sequencing to examine the microbial communities in the rhizosphere of spring wheat and radish grown in greenhouse experiments on Chernevaya and control soils. Additionally, representative fungal strains were isolated and assessed for their ability to promote growth in wheat seedlings. The study revealed that the most abundant phyla in the rhizospheric fungal community were Mortierellomycota, primarily consisting of Mortierella species, and Ascomycota. Mucor and Umbelopsis comprised the majority of Mucoromycota in the control soils. Fusarium and Oidiodendron, two potentially plant-pathogenic fungi, were only found in the rhizosphere of crops grown in the control soil. Conversely, Chernevaya soil contained a diverse range of potential biocontrol fungi for plants. Tested novel fungal isolates showed a stimulating effect on the development of wheat seedlings and positively affected their rate of biomass accumulation. The results of the study demonstrate that the soil of Chernevaya taiga do indeed contain fungi with prominent potential to stimulate agricultural plants growth.
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The sequence data presented in this study are openly available in Figshare at https://doi.org/10.6084/m9.figshare.22639870.v1 (accessed on 14 April 2023). All other data generated or analysed during this study are included in this article.
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Acknowledgements
The study was carried out using the equipment of the resource center “Genomic Technologies, Proteomics and Cell Biology” of ARRIAM and core facilities “Bioengineering” and “UNIQEM collection” of Research Center of biotechnology RAS. The authors are extremely grateful to Prof. Dmitrii Vlasov, Saint Petersburg State University, Department of Botany, for reading and discussing this manuscript and for insightful remarks.
Funding
This research is funded in part by the Saint Petersburg State University (grant ID 94030965). The data analysis was carried out using computational resources provided by the Resource Center “Computer Center of SPbU”. This work was funded by the Russian Science Foundation, grant number 19-16-00049 (field work, 16S rRNA genes amplification, sequencing), by Saint Petersburg State University, grant ID PURE 93023187 (DNA sequencing analyses, phylogenetic analysis) and by the Ministry of Science and Higher Education of the Russian Federation, state task registration number 122040800164-6 (microbiological analyses, pot experiments, isolation, and analysis of fungal strains).
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All authors contributed to the study concept and design. Experimentation was performed by IK, ET, AT. The first draft was written by IK, MR, SS and AL, and all authors commented on the previous version of the manuscript. All authors read and approved the final manuscript.
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Kravchenko, I., Rayko, M., Sokornova, S. et al. Analysis of rhizosphere fungal community of agricultural crops cultivated in laboratory experiments on Chernevaya taiga soil. World J Microbiol Biotechnol 40, 27 (2024). https://doi.org/10.1007/s11274-023-03827-6
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DOI: https://doi.org/10.1007/s11274-023-03827-6