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Imidacloprid degrading efficiency of Pseudomonas plecoglossicida MBSB-12 isolated from pesticide contaminated tea garden soil of Assam

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Abstract

Long-term use of toxic pesticides in agricultural grounds has led to adverse effects on the environment and human health. Microbe-mediated biodegradation of pollutants is considered an effective strategy for the removal of contaminants in agricultural and environmental sustainability. Imidacloprid, a neonicotinoid class of pesticides, was widely applied insecticide in the control of pests in agricultural fields including the tea gardens of Assam. Here, native bacteria from imidacloprid contaminating tea garden soils were isolated and screened for imidacloprid degradation efficiency under laboratory conditions. Out of the 30 bacterial isolates, 4 were found to tolerate high concentrations of imidacloprid (25,000 ppm), one of which isolate MBSB-12 showed the highest efficiency for imidacloprid tolerance and utilization as the sole carbon source. Morphological, biochemical, and 16 S ribosomal RNA gene sequencing-based characterization revealed the isolate as Pseudomonas plecoglossicida MBSB-12. The isolate reduced 87% of extractable imidacloprid from the treated soil in 90 days compared to the control soil (without bacterial treatment). High-Resolution Mass Spectrometry (HRMS) analysis indicated imidacloprid breakdown to comparatively less harmful products viz., imidacloprid guanidine olefin [m/z = 209.0510 (M + H)+], imidacloprid urea [m/z = 212.0502 (M + H)+] and a dechlorinated degraded product of imidacloprid with m/z value 175.0900 (M + H)+. Further investigation on the molecular machinery of P. plecoglossicida MBSB-12 involved in the degradation of imidacloprid is expected to provide a better understanding of the degradation pathway.

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Acknowledgements

The authors are grateful to the Department of Agricultural Biotechnology, Assam Agricultural University and DBT - North East Centre for Agricultural Biotechnology, Assam Agricultural University for providing the required facilities and laboratory space for conducting the research works. The authors are also thankful to CSIR-North East Institute of Science and Technology, Jorhat, Assam for providing the instrumentation facility for HRMS analysis. Special thanks to Dr. Rajiv Das Kangabam for his kind help and suggestions.

Funding

SB received scholarship from Department of Biotechnology, Ministry of Science and Technology, Government of India through DBT-North East Centre for Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Faculty of Agriculture, Assam Agricultural University, 785013, Jorhat, Assam, India

    Subangshi Borah, Dibya Jyoti Hazarika, Manjistha Baruah, Robin Chandra Boro & Madhumita Barooah

  2. DBT-North East Centre for Agricultural Biotechnology, Assam Agricultural University, 785013, Jorhat, Assam, India

    Sudipta Sankar Bora

  3. Department of Tea Husbandry and Technology, Assam Agricultural University, 785013, Jorhat, Assam, India

    Manuranjan Gogoi

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  1. Subangshi Borah
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Contributions

MB* conceived the idea; MB*, SB and DJH designed the study; MG supervised the sampling; SB performed the experimental analyses, prepared the draft manuscript and the figures; SB, DJH, MB and SSB performed data analysis and instrumentation support; MB* and RCB provided technical supervision during the study; DJH, SSB, RCB and MB* critically revised the manuscript; All authors have contributed, read and agreed to the content of the of the manuscript.

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Correspondence to Madhumita Barooah.

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Borah, S., Hazarika, D.J., Baruah, M. et al. Imidacloprid degrading efficiency of Pseudomonas plecoglossicida MBSB-12 isolated from pesticide contaminated tea garden soil of Assam. World J Microbiol Biotechnol 39, 59 (2023). https://doi.org/10.1007/s11274-022-03507-x

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