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Evaluation of microbial population dynamics in the co-composting of cow manure and rice straw using high throughput sequencing analysis

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Abstract

Microbial population dynamics in co-composting of cow manure and rice straw were evaluated using 16S high throughput sequencing technology. Physicochemical factors, including temperature, pH, nitrogen contents, the ratio of carbon and nitrogen, and germination index, were also determined in this study. 16S high throughput sequencing results showed that bacterial community structure and composition significantly varied in each phase of composting. The major phyla included Bacteroidetes, Proteobacteria, Firmicutes, Actinobacteria and Planctomycetes, respectively. Bacteroidetes and Proteobacteria were the most abundant phyla in all phases, and Actinobacteria was just dominant in the mesophilic phase, while Firmicutes and Planctomycetes were ubiquitous. At the genus level, Simiduia, Flavobacterium, unclassified Chitinophagaceae and Flexibacter notably changed in each phase of composting. Bacterial community diversity in the mesophilic phase was higher than that in others based on the Shannon–Wiener index and Simpson diversity index. The ratio of carbon and nitrogen and germination index indicated that the co-composting of cow manure and rice straw reached maturation. The result of nitrogen contents showed that nitrogen loss mainly occurred in the thermophilic phase. In addition, the differences in the distributions of key OTUs between in the late thermophilic phase and the cooling and maturation phase were unobvious compared with other phase’s base on the principal component analysis. Redundancy analysis revealed that the changes of nitrogen played a predominant role in the distributions of OTUs during the composting process.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (31272484 and 31372351).

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Authors and Affiliations

  1. Resources and Environmental College, Northeast Agricultural University, Harbin, 150030, China

    Guangming Ren, Xiuhong Xu, Juanjuan Qu, Liping Zhu & Tingting Wang

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  1. Guangming Ren
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  2. Xiuhong Xu
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Correspondence to Xiuhong Xu.

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Ren, G., Xu, X., Qu, J. et al. Evaluation of microbial population dynamics in the co-composting of cow manure and rice straw using high throughput sequencing analysis. World J Microbiol Biotechnol 32, 101 (2016). https://doi.org/10.1007/s11274-016-2059-7

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