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Isolation, diversity and acetylcholinesterase inhibitory activity of the culturable endophytic fungi harboured in Huperzia serrata from Jinggang Mountain, China

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Abstract

Huperzia serrata has many important medicinal properties with proven pharmacological potential. Some of these properties may be mediated by its endophytic fungi. To test this hypothesis, in the present study, we provided a first insights into evaluating the species composition and acetylcholinesterase (AChE) inhibitory activity of the culturable endophytic fungi of H. serrata from the regional at Jinggang Mountain in southeastern China. A total number of 885 fungal isolates distributed across 44 genera and 118 putative species were obtained from 1422 fragments of fine H. serrata roots, stems and leaves base on ITS-rDNA sequences BLAST analysis. The endophytic fungi were phylogenetically diverse and species-rich, with high rate of colonization and isolation. The assemble of endophytic fungi consisted mainly of Ascomycota (97.15 %), followed by Basidiomycota (1.92 %) and unknown fungal species (0.90 %). Colletotrichum (64.29 %), Phyllosticta (3.39 %), Hypoxylon (2.81 %), Xylaria (2.25 %) and Nigrospora (2.04 %) were the most abundant genera, whereas the remaining genera were infrequent groups. Although, roots yielded low abundance strains, the diverse and species-rich were both higher than that of stems and leaves. In addition, out of the 247 endophytic fungi strains determinated, 221 fungal extracts showed AChE inhibition activities in vitro. Among them, 22 endophytic fungi strains achieved high inhibitory activity (≥50 %) on AChE which belongs to 13 genera and five incertae sedis strains. Four endophytic fungi designated as JS4 (Colletotrichum spp.), FL14 (Ascomycota spp.), FL9 (Sarcosomataceae spp.) and FL7 (Dothideomycetes spp.) were displayed highly active (≥80 %) against AChE, which the inhibition effects were even more intense than the positive control. Our findings highlight that H. serrata grown in Jinggang Mountain harbors a rich and fascinating endophytic fungus community with potential AChE inhibitory activity, which could further broaden the natural acetylcholinesterase inhibitors resources used for Alzheimer’s disease treatment.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 81260617, 31300051), by the Major Science and technology Subject of Jiangxi Province (No. 2010BSA19200, 2010AZD00307), by the Natural Science Foundation of Jiangxi Province of China (No. 20142BAB214020) and by the Foundation of Jiangxi Educational Committee (No. GJJ14595).

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    1. Key Laboratory of Bioprocess Engineering of Jiangxi Province, College of life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330013, China

      Ya Wang & Du Zhu

    2. Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China

      Zheng Lai, Xi-Xi Li, Ri-Ming Yan, Zhi-Bin Zhang, Hui-Lin Yang & Du Zhu

    3. Science and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330025, China

      Xi-Xi Li

    4. Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Yichun, 336000, China

      Ya Wang

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    Ya Wang and Zheng Lai have contributed equally to this work.

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    Wang, Y., Lai, Z., Li, XX. et al. Isolation, diversity and acetylcholinesterase inhibitory activity of the culturable endophytic fungi harboured in Huperzia serrata from Jinggang Mountain, China. World J Microbiol Biotechnol 32, 20 (2016). https://doi.org/10.1007/s11274-015-1966-3

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