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Characterization of an enantioselective amidase with potential application to asymmetric hydrolysis of (R, S)-2, 2-dimethylcyclopropane carboxamide

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Abstract

Amidase is a promising synthesis tool for chiral amides and related derivatives. In the present study, the biochemical properties of the Delftia tsuruhatensis CCTCC M 205114 enantioselective amidase were determined for its potential application in chiral amides synthesis. D. tsuruhatensis CCTCC M 205114 amidase was purified 105.2 fold with total activity recovery of 4.26%. The enzyme is a monomer with a subunit of approximately 50 kDa by analytical gel filtration HPLC and SDS–PAGE. It had a broad substrate spectrum and displayed high enantioselectivity against R-2, 2-dimethylcyclopropane carboxamide and R-mandelic amide. The amidase was applied to enantioselective hydrolysis of R-2, 2-dimethylcyclopropane carboxamide from racemic (R, S)-2, 2-dimethylcyclopropane carboxamide to accumulate S-2, 2-dimethylcyclopropane carboxamide. This enzyme did not require metal ions for the hydrolysis reaction. Its optimal pH and temperature were 8.0 and 35°C, respectively. The K m and V max of the amidase for R-2, 2-dimethylcyclopropane carboxamide were 2.54 mM and 8.37 μmol min−1 mg protein−1, respectively. After 60 min of the reaction, R-2, 2-dimethylcyclopropane carboxamide was completely hydrolyzed, generating S-2, 2-dimethylcyclopropane carboxamide with a yield of 45.9% and an e.e. of above 99%. Therefore, this amidase can serve as a promising producer for S-2, 2-dimethylcyclopropane carboxamide and other amides.

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Acknowledgments

We would like to thank Elise A. Lamonta (Department of Veterinary Population Medicine, University of Minnesota) for reviewing and valuable suggestions. This work was supported by the Major Basic Research Development Program of China (No. 2011CB710800) and the Natural Scientific Foundation of Zhejiang (Y407225 and Y4080334).

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Authors and Affiliations

  1. Institute of Bioengineering, Zhejiang University of Technology, No. 18, Chaowang Road, 310032, Hangzhou, Zhejiang Province, People’s Republic of China

    Yuan-Shan Wang, Feng Cheng, Ren-Chao Zheng, Ya-Jun Wang & Yu-Guo Zheng

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  1. Yuan-Shan Wang
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  2. Feng Cheng
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  3. Ren-Chao Zheng
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Correspondence to Yu-Guo Zheng.

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Wang, YS., Cheng, F., Zheng, RC. et al. Characterization of an enantioselective amidase with potential application to asymmetric hydrolysis of (R, S)-2, 2-dimethylcyclopropane carboxamide. World J Microbiol Biotechnol 27, 2885–2892 (2011). https://doi.org/10.1007/s11274-011-0769-4

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