Abstract
The new in situ seed baiting method using seed packets to assess germination of orchid species within soil provides a means of locating, collecting, and identifying specific fungi that are involved in the lifecycle of orchids in the wild and investigating their relationship with the orchids under natural field conditions. Two isolates (SHH44, SHH53) originating from the seedlings found in the seeds packets in situ were demonstrated to support seed germination and seedling development (in vitro) of two endangered Chinese endemic herbs, Dendrobium officinale and Dendrobium nobile (Orchidaceae). Advanced protocorm development (Stage 3 and greater) in this study of these species of Dendrobium only occurred under the 12/12 h L/D photo-period indicated that illumination may play an important role in seedling recruitment of terrestrial orchid species in their natural environment. The information provided in this study may prove invaluable in the conservation of D. officinale and D. nobile and congener species in China, especially given their rare status.
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Acknowledgments
We would like to thank Thomas Avery Garran for his helpful advice and hard work in the preparation of this manuscript. Financial support was provided by the Chinese National Natural Science Fund (No. 30770068, 30900004), and the National High Technology Research and Development Program of China (No. 2008AA09Z405).
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Wang, H., Fang, H., Wang, Y. et al. In situ seed baiting techniques in Dendrobium officinale Kimuraet Migo and Dendrobium nobile Lindl. : the endangered Chinese endemic Dendrobium (Orchidaceae). World J Microbiol Biotechnol 27, 2051–2059 (2011). https://doi.org/10.1007/s11274-011-0667-9
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DOI: https://doi.org/10.1007/s11274-011-0667-9