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Molecular characterization and mating type analysis of oyster mushroom (Pleurotus spp.) using single basidiospores for strain improvement

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Abstract

Existence of variability in morphological traits and growth rate of mycelium of homokaryotic single basidiospores can be exploited for the development of inter-strainal hybrids. We isolated 182 single basidiospores from mushroom bodies of P. sajor-caju, P. florida, P. eous and one wild relative of Pleurotus called Hypsizygus ulmaris. The single spores were isolated using a new technique that is less prone to contamination and more efficient than the common techniques used by earlier workers. All the isolates showed a varied range of cultural morphology. Mating types of all the isolates within the species were identified on the basis of hyphal fusion via anastomosis with the tester strains. Four compatible pairs of isolates with well prominent tuft in the contact zone were selected for dikaryon isolation. Dikaryons were used for spawn preparation and mushroom cultivation. The dikaryotic isolates with their replicates were evaluated for spawn run period, yield and biological efficiency. 42 isolates (10 di- and 32 mono-karyotic isolates) were analyzed with RAPD genetic markers. Phenotypic characters and mating types of all the 42 isolates analyzed genetically were correlated with their genetic polymorphism data. The isolates showed very large diversity both at the phenotypic and the genotypic level. Available phenotypic and genotypic data can further help in the selection of monosporous isolates for developing inter-strainal hybrids which can lead to better prospects for genetic improvement in different species of Pleurotus.

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Acknowledgments

We thank all the staff members of mushroom division in IGKVV Raipur (C.G.), India for helping in the spawn preparation and mushroom cultivation.

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Authors and Affiliations

  1. Evolutionary Genomics and Bioinformatics Laboratory, Division of Genomics and Bioinformatics, National Institute of Malaria Research (NIMR), Sector-8, Dwarka, New Delhi, 110 077, India

    Bhavna Gupta

  2. Vector Control Division, National Institute of Malaria Research (NIMR), Sector-8, Dwarka, New Delhi, 110 077, India

    B. P. Niranjan Reddy

  3. Department of Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.), 492006, India

    Anil S. Kotasthane

Authors
  1. Bhavna Gupta
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  2. B. P. Niranjan Reddy
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  3. Anil S. Kotasthane
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Correspondence to Bhavna Gupta.

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Bhavna Gupta and B.P. Niranjan Reddy contributed equally.

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Gupta, B., Niranjan Reddy, B.P. & Kotasthane, A.S. Molecular characterization and mating type analysis of oyster mushroom (Pleurotus spp.) using single basidiospores for strain improvement. World J Microbiol Biotechnol 27, 1–9 (2011). https://doi.org/10.1007/s11274-010-0419-2

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  • DOI: https://doi.org/10.1007/s11274-010-0419-2

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