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Heterologous expression of a hydrogenase gene in Enterobacter aerogenes to enhance hydrogen gas production

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Abstract

The hydrogenase gene from Enterobacter cloacae (IIT-BT 08) was amplified and inserted into a prokaryotic expression vector to create a recombinant plasmid (pGEX-4T-2-Cat/hydA). The recombinant plasmid was transformed into a hydrogen-producing strain of Enterobacter aerogenes (ATCC13408). SDS–PAGE and western blot analysis confirmed the successful expression of the GST-tagged hydA protein. Anaerobic fermentation for the production of hydrogen from glucose was investigated using E. aerogenes ATCC13408 and the recombinant strain. The results showed that the hydrogen yield markedly increased, from 442.82 ± 22.61 ml/g glucose in the ATCC13408 strain to 864.02 ± 36.8 ml/g glucose in the recombinant. The maximum rate of hydrogen production was found to be 53.49 ± 3.34 ml l−1 h−1 using 1% (w/v) glucose as the substrate at pH 6.0 and a reaction temperature of 37°C.

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Acknowledgments

This project was supported by the National Program of High-tech Research and Development of China (863 High-Tech Program, No. 2006AAO5Z122).

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Authors and Affiliations

  1. College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, China

    Jin-Fang Zhao & Chuan-Xi Zhang

  2. State Key Lab of Clean Energy Utilization, Zhejiang University, 310027, Hangzhou, China

    Wen-Lu Song & Jun Cheng

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  1. Jin-Fang Zhao
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  2. Wen-Lu Song
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  3. Jun Cheng
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  4. Chuan-Xi Zhang
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Correspondence to Chuan-Xi Zhang.

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Zhao, JF., Song, WL., Cheng, J. et al. Heterologous expression of a hydrogenase gene in Enterobacter aerogenes to enhance hydrogen gas production. World J Microbiol Biotechnol 26, 177–181 (2010). https://doi.org/10.1007/s11274-009-0139-7

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  • DOI: https://doi.org/10.1007/s11274-009-0139-7

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