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Isolation and Characterization of A Metal-resistant Pseudomonas Aeruginosa Strain

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Summary

The use of microorganisms to remove heavy metals from industrial effluent is an area of extensive research and development. Attempts have been made to isolate and characterize metal-resistant microorganisms from treated oil mill industry effluent wastewater samples. The metal-resistant organisms that showed values of minimum inhibitory concentration towards metals (Cd, Cr, Ni and Pb) ranging from 100 to 800 ppm level were screened. A potent metal-resistant organism, isolate BC15 from the wastewater samples was tentatively identified as Pseudomonas sp. Detailed analysis of morphological, biochemical and 16S rDNA sequence of the isolate revealed that it is closely related to Pseudomonas aeruginosa (94%). Pseudomonas BC15 was capable of absorbing 93% Ni, 65% Pb, 50% Cd and 30% Cr within 48 h from the medium containing 100 mg of each heavy metal per liter. The multiple metal tolerance of this strain was also associated with resistance to antibiotics such as ampicillin, tetracycline, chloramphenicol, erythromycin, kanamycin and streptomycin.

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Abbreviations

LB:

Luria–Bertani

AFM:

Atomic force microscopy

ppm:

Parts per million

AAS:

Atomic absorption spectrophotometer

rpm:

Revolution per minute

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Authors and Affiliations

  1. Department of Biochemistry, Center for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, 625 021, Madurai, India

    Chelliah Edward Raja, Kolandaswamy Anbazhagan & Govindan Sadasivam Selvam

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  1. Chelliah Edward Raja
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  2. Kolandaswamy Anbazhagan
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  3. Govindan Sadasivam Selvam
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Correspondence to Govindan Sadasivam Selvam.

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Raja, C.E., Anbazhagan, K. & Selvam, G.S. Isolation and Characterization of A Metal-resistant Pseudomonas Aeruginosa Strain. World J Microbiol Biotechnol 22, 577–585 (2006). https://doi.org/10.1007/s11274-005-9074-4

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  • DOI: https://doi.org/10.1007/s11274-005-9074-4

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