Summary
Process conditions for cell cultures derived from conchocelis of female red macroalga Porphyra haitanensis were optimized in an illuminated 0.3-l bubble-column photobioreactor, using CO2 in air as the sole carbon source during a 20-day cultivation period. It reached the highest growth rate when the initial cell density was 700 mg l−1 (dry weight), the optional aeration rate was 1.2 v/v/min, inorganic nitrate concentration was 15 mM and inorganic phosphate concentration was 0.6 mM. This is the first reported bioreactor cultivation study of cell cultures derived from conchocelis of Porphyra haitanensis.
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Acknowledgments
This work was supported by the grant from the Key Innovative Project of the Chinese Academy of Sciences (No. KZCX3-SW-215). The authors also thank Dr. JiangPeng for helpful discussions and Dr. YangRui for providing Porphyra haitanensis female conchocelis cells in this research.
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Zhang, W., Gao, JT., Zhang, YC. et al. Optimization of conditions for cell cultivation of Porphyra haitanensis conchocelis in a bubble-column bioreactor. World J Microbiol Biotechnol 22, 655–660 (2006). https://doi.org/10.1007/s11274-005-9050-z
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DOI: https://doi.org/10.1007/s11274-005-9050-z