Summary
A Chlamydomonas reinhardtii (C. reinhardtii) chloroplast expression vector, papc-B, containing the apc-B gene that encodes the beta subunit of the light-harvesting antenna protein allophycocyanin (APC) of cyanobacteria, was constructed and transferred to the chloroplast genome of C. reinhardtii by the biolistic method. The transformants were identified by Southern blot, Western blot and ELISA assays after selection on resistant medium. The recombinant APC beta subunit was expressed in the C. reinhardtii chloroplast and accounted for up to 2–3% (w/w) of the total soluble protein (TSP), suggesting a promising prospect of using C. reinhardtii chloroplasts to produce functional plant-derived proteins.
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References
Bateman J.M., Purton S., 2000 Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker Molecular Genetics and Genomics 263: 404–410
Franklin S.E., Mayfield S.P., 2004 Prospects for molecular farming in the green alga Chlamydomonas reinhardtii Current Opinion in Plant Biology 7: 159–165
Goldschmidt-Clermont M., 1991 Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker for site-directed transformation of Chlamydomonas Nucleic Acids Research 19: 4083–4089
Harris E., 1989 The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use. Academic Press San Diego pp. 31–52 ISBN 012326880X
Kindle K.L., Richards K.L., Stem D.B., 1991 Engineering the chloroplast genome: techniques and capabilities for chloroplast transformation in Chlamydomonas reinhardtii Proceedings of the National Academy of Sciences of the United States of America 88: 1721–1725
Maccoll R., 1998 Cyanobacterial phycobilisomes Journal of Structure Biology 124: 311–334
Qin S., 1998 Cloning and sequencing of the allophycocyanin genes from Spirulina maxima (Cyanophyta) Chinese Journal of Oceanology and Limnology 16(Suppl): 6–11
Sun M., Qian K.X., Su N., Chang C.H., Liu J.X., Shen G.F., 2003 Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast Biotechnology Letters 25: 1087–1092
Tang Z.H., Zhao F.Q., Qin S., Wu S.J., Gao J.T., Lin L.P., 2004 The production of rAPC and the study of its inhibition effect on H22 tumor High Technology Letters (Chinese) 3: 83–86
Zhao F.Q., Tang Z.H., Lin F., Bao L., Qin S., Dou C.G., 2003 High Cell Density Fermentation of a Recombinant E. coli Strain P1 Producing an Anti-S180 Tumor Recombinant Protein (rAPC) High Technology Letters (Chinese) 2: 29–33
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This research was supported by grants from Key Innovative Project (KZCX-3-SW-215).
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Su, Z., He, D., Qian, K. et al. The recombination and expression of the allophycocyanin beta subunit gene in the chloroplast of Chlamydomonas reinhardtii . World J Microbiol Biotechnol 22, 101–103 (2006). https://doi.org/10.1007/s11274-005-9001-8
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DOI: https://doi.org/10.1007/s11274-005-9001-8