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Fungal and Bacterial Co-Bioaugmentation of a Pesticide-Degrading Biomixture: Pesticide Removal and Community Structure Variations during Different Treatments

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Abstract

Biopurification systems (BPS) are employed for the treatment of pesticide-containing wastewaters. In this work, a biomixture (active core of BPS) complemented by the addition of the fungus Trametes versicolor was evaluated for the elimination of a mixture of pesticides under different treatment conditions. The biomixture achieved high removal of all the pesticides assayed after 16 d: atrazine (68.4%, t1/2: 9.6 d), carbendazim (96.7%, t1/2: 3.6 d), carbofuran (98.7%, t1/2: 3.1 d) and metalaxyl (96.7%, t1/2: 3.8 d). Variations in the treatment conditions including addition of the antibiotic oxytetracycline and co-bioaugmentation with a bacterial consortium did not significantly affect the removal performance of the biomixture. Bacterial and fungal community profiles determined by DGGE analyses revealed changes that responded to biomixture aging, and not to antibiotic or pesticide addition. The proposed biomixture exhibits very efficient elimination during simultaneous pesticide application; moreover, the matrix is highly stable during stressful conditions such as the co-application of antibiotics of agricultural use.

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Acknowledgements

The authors acknowledge Vicerrectoría de Investigación, Universidad de Costa Rica (projects 802-B4-503 and 802-B6-137), and the Costa Rican Ministry of Science, Technology and Telecommunications, MICITT (project FI-093-13).

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Authors and Affiliations

  1. Centro de Investigación en Contaminación Ambiental (CICA), Universidad de Costa Rica, San José, 2060, Costa Rica

    Víctor Castro-Gutiérrez, Mario Masís-Mora, Elizabeth Carazo-Rojas & Carlos E. Rodríguez-Rodríguez

  2. Centro de Investigación en Biología Celular y Molecular (CIBCM), Universidad de Costa Rica, San José, 2060, Costa Rica

    Víctor Castro-Gutiérrez & Marielos Mora-López

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  1. Víctor Castro-Gutiérrez
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  2. Mario Masís-Mora
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  5. Carlos E. Rodríguez-Rodríguez
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Castro-Gutiérrez, V., Masís-Mora, M., Carazo-Rojas, E. et al. Fungal and Bacterial Co-Bioaugmentation of a Pesticide-Degrading Biomixture: Pesticide Removal and Community Structure Variations during Different Treatments. Water Air Soil Pollut 230, 247 (2019). https://doi.org/10.1007/s11270-019-4282-y

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