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Comparison Between New Wild Plant Trifolium repens and Vicia faba on their Sensitivity in Detecting the Genotoxic Potential of Heavy Metal Solutions and Heavy Metal-Contaminated Soils

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Abstract

The DNA damage and cytotoxicity induced by CdCl2 solutions and soils anthropogenically contaminated with heavy metals were studied using the micronucleus (MN) test. Vicia faba, a plant model widely used in liquid exposure assays, was adapted for direct exposure to a solid phase. In addition, the MN assay was adapted to a new wild plant system, the white clover (Trifolium repens). The results obtained after exposure to CdCl2 solutions confirmed that V. faba root cells were a sensitive model and revealed that T. repens root cells were not appropriate for the detection of micronuclei (although they were highly sensitive to the cytotoxic effect of CdCl2). Concerning the results observed after direct exposure to contaminated soils (solid-phase exposure), the MN frequency scores in V. faba root cells were increased in a statistically significant and dose-related manner compared to the control plants. Regarding T. repens root cells, this solid phase exposure confirmed that this model is not appropriate for use in the micronucleus assay.

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Acknowledgements

We gratefully thank the French government (ANR funds) for their financial support for this research program, which was also subsidised by the European Regional Development fund. We express our sincere thanks to Mike Howsam for significant revision of the English.

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Authors and Affiliations

  1. Institut Lillois d’Ingénierie de la Santé (ILIS), Université Droit et Santé de Lille, 42 rue Ambroise Paré, 59120, Loos, France

    Nicolas Manier & Annabelle Deram

  2. Département de Botanique, Université Droit et Santé de Lille, EA 2690, B.P. 83, 59006, Lille cedex, France

    Nicolas Manier & Annabelle Deram

  3. Département Toxicologie-Santé Publique-Environnement, Université Droit et Santé de Lille, EA 2690, B.P. 83, 59006, Lille cedex, France

    Frank Le Curieux & Daniel Marzin

  4. Laboratoire de Toxicologie Génétique, Institut Pasteur de Lille, EA 2690, 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France

    Frank Le Curieux & Daniel Marzin

  5. Institut Lillois d’Ingénierie de la Santé (ILIS), Université de Lille 2, 42, rue Ambroise Paré, 59120, Loos, France

    Nicolas Manier

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  1. Nicolas Manier
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Correspondence to Nicolas Manier.

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Manier, N., Deram, A., Le Curieux, F. et al. Comparison Between New Wild Plant Trifolium repens and Vicia faba on their Sensitivity in Detecting the Genotoxic Potential of Heavy Metal Solutions and Heavy Metal-Contaminated Soils. Water Air Soil Pollut 202, 343–352 (2009). https://doi.org/10.1007/s11270-009-9981-3

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  • DOI: https://doi.org/10.1007/s11270-009-9981-3

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