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A novel bluetongue virus serotype 2 strain isolated from a farmed Florida white-tailed deer (Odocoileus virginianus) arose from reassortment of gene segments derived from co-circulating serotypes in the Southeastern USA

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Abstract

Bluetongue disease is a reportable animal disease that affects wild and farmed ruminants, including white-tailed deer (WTD). This report documents the clinical findings, ancillary diagnostics, and genomic characterization of a novel reassortant bluetongue virus serotype 2 (BTV-2) strain isolated from a dead Florida farmed WTD in 2022. Our analyses support that this BTV-2 strain likely stemmed from the acquisition of genome segments from co-circulating BTV strains in Florida and Louisiana. In addition, our analyses also indicate that genetically uncharacterized BTV strains may be circulating in the Southeastern USA; however, the identity and reassortant status of these BTV strains cannot be determined based on the VP2 and VP5 genome sequences. Hence, continued surveillance based on complete genome characterization is needed to understand the genetic diversity of BTV strains in this region and the potential threat they may pose to the health of deer and other ruminants.

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Data availability

The complete gene coding sequences for all 10 segments of the genomes recovered in this study have been deposited in the NCBI GenBank database and are available under the GenBank accession nos. OR672561-OR672570.

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Funding

This research was funded by the University of Florida, Institute for Food and Agricultural Sciences, Cervidae Health Research Initiative, funded through the Florida State Legislature, grant number LBR2199.

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Authors and Affiliations

  1. Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL, 32610, USA

    Pedro H. O. Viadanna, Austin Surphlis & Kuttichantran Subramaniam

  2. Emerging Pathogens Institute, University of Florida, 2055 Mowry Rd, Gainesville, FL, 32610, USA

    Pedro H. O. Viadanna, Austin Surphlis, Kristen N. Wilson, Zoe S. White, Emily DeRuyter, Tracey D. Logan, John A. Lednicky, Samantha M. Wisely & Kuttichantran Subramaniam

  3. Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL, 32610, USA

    An-Chi Cheng, Catherine E. Dixon, Sarah Meisner & Juan M. C. Krauer

  4. Department of Wildlife Ecology and Conservation, University of Florida, 110 Newins-Ziegler Hall, Gainesville, FL, 32611, USA

    Kristen N. Wilson, Zoe S. White & Samantha M. Wisely

  5. Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, 1225 Center Dr, Gainesville, FL, 32610, USA

    Emily DeRuyter, Tracey D. Logan & John A. Lednicky

Authors
  1. Pedro H. O. Viadanna
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Contributions

Conceptualization: PHOV, JAL, SMW, KS. Methodology: PHOV, JMCK, JAL, SMW, KS. Formal analysis: PHOV, AS, ACC, CED, SM, KNW, ZSW, ED, TDL, JMCK. Investigation: PHOV, AS, ACC, CED, SM, KNW, ZSW, ED, TDL, KS. Resources: JMCK, JAL, SMW, KS. Writing—original draft: PHOV, AS, JAL, KS. Writing—review & editing: PHOV, AS, ACC, CED, SM, KNW, ZSW, ED, TDL, JMCK, JAL, SMW, KS. Visualization: PHOV, AS, KS. Supervision: KS. Project administration: SMW, KS. Funding acquisition: SMW, KS.

Corresponding author

Correspondence to Kuttichantran Subramaniam.

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The authors declare that they have no competing interests.

Ethical approval

This work was approved by the University of Florida Institutional Animal Care and Use Committee (IACUC) Protocol Numbers 201609390 and 201909390.

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Edited by Juergen Richt.

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Viadanna, P.H.O., Surphlis, A., Cheng, AC. et al. A novel bluetongue virus serotype 2 strain isolated from a farmed Florida white-tailed deer (Odocoileus virginianus) arose from reassortment of gene segments derived from co-circulating serotypes in the Southeastern USA. Virus Genes 60, 100–104 (2024). https://doi.org/10.1007/s11262-023-02047-2

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