Skip to main content
SpringerLink
Log in

Genetic diversity of 3′ region of glycoprotein D gene of bovine herpesvirus 1 and 5

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are closely related alphaherpesviruses of cattle. While BoHV-1 is mainly associated with respiratory/genital disease and rarely associated with neurological disease, BoHV-5 is the primary agent of meningoencephalitis in cattle. The envelope glycoprotein D of alphaherpesviruses (BoHV-1/gD1 and BoHV-5/gD5) is involved in the early steps of virus infection and may influence virus tropism and neuropathogenesis. This study performed a sequence analysis of the 3′ region of gD gene (gD3′) of BoHV-1 isolates recovered from respiratory/genital disease (n = 6 and reference strain Cooper) or from neurological disease (n = 7); and from seven typical neurological BoHV-5 isolates. After PCR amplification, nucleotide (nt) sequencing, and aminoacid (aa) sequence prediction; gD3′ sequences were compared, identity levels were calculated, and selective pressure was analyzed. The phylogenetic reconstruction based on nt and aa sequences allowed for a clear differentiation of BoHV-1 (n = 14) and BoHV-5 (n = 7) clusters. The seven BoHV-1 isolates from neurological disease are grouped within the BoHV-1 branch. A consistent alignment of 346 nt revealed a high similarity within each viral species (gD1 = 98.3 % nt and aa; gD5 = 97.8 % nt and 85.8 % aa) and an expected lower similarity between gD1 and gD5 (73.7 and 64.1 %, nt and aa, respectively). The analysis of molecular evolution revealed an average negative selection at gD3′. Thus, the phylogeny and similarity levels allowed for differentiation of BoHV-1 and BoHV-5 species, but not further division in subspecies. Sequence analysis did not allow for the identification of genetic differences in gD3′ potentially associated with the respective clinical/pathological phenotypes, yet revealed a lower level of gD3′ conservation than previously reported.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. B. Roizman, R.C. Desrosiers, B. Fleckenstein, C. Lopez, A.C. Minson, M.J. Studdert, Arch. Virol. 123, 425–449 (1992)

    Article  Google Scholar 

  2. M. Engels, E. Loepfe, P. Wild, E. Schraner, R. Wyler, J. Gen. Virol. 68, 2019–2023 (1987)

    Article  CAS  PubMed  Google Scholar 

  3. G. Delhon, M.P. Moraes, Z. Lu, C.L. Afonso, E.F. Flores, R. Weiblen, G.F. Kutish, D.L. Rock, J. Virol. 77, 10339–10347 (2003)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. E.B. Belknap, J.K. Collins, V.K. Ayers, P.C. Schultheiss, Vet. Pathol. 31, 358–365 (1994)

    Article  CAS  PubMed  Google Scholar 

  5. T.J. Bagust, T.J. J. Comp. Pathol. 82, 365–374 (1972)

    Article  CAS  PubMed  Google Scholar 

  6. R. Weiblen, C.S. de Barros, T.F. Canabarro, I.E. Flores, Vet. Rec. 124, 666–667 (1989)

    Article  CAS  PubMed  Google Scholar 

  7. T. Crook, J. Benavides, G. Russell, J. Gilray, M. Maley, K. Willoughby, J. Vet. Diagn. Invest. 24, 662–670 (2012)

    Article  PubMed  Google Scholar 

  8. H. Guarino, A. Nunez, M.V. Repiso, A. Gil, D.A. Dargatz, Prev. Vet. Med. 85, 34–40 (2008)

    Article  CAS  PubMed  Google Scholar 

  9. S.C. Salvador, R.A.A. Lemos, F. Riet-Correa, P.M. Roehe, A.L.A.R. Osório, Braz. J. Vet. Res. 18, 75–82 (1998)

    Google Scholar 

  10. R.C. D’Arce, R.S. Almeida, T.C. Silva, A.C. Franco, F. Spilki, P.M. Roehe, C.W. Arns, Vet. Microbiol. 88, 315–324 (2002)

    Article  PubMed  Google Scholar 

  11. A.E. Metzler, A.A. Schudel, M. Engels, Arch. Virol. 87, 205–217 (1986)

    Article  CAS  PubMed  Google Scholar 

  12. F.A. Rijsewijk, M.J. Kaashoek, J.P. Langeveld, R. Meloen, J. Judek, K. Bienkowska-Szewczyk, M.A. Maris-Veldhuis, J.T. van Oirschot, J. Gen. Virol. 80, 1477–1483 (1999)

    CAS  PubMed  Google Scholar 

  13. F.R. Spilki, P.A. Esteves, A.D. da Silva, A.C. Franco, F.A. Rijsewijk, P.M. Roehe, J. Virol. Methods 129, 191–193 (2005)

    Article  CAS  PubMed  Google Scholar 

  14. C.K. Traesel, M.S. Silva, F.R. Spilki, R. Weiblen, E.F. Flores, Res. Vet. Sci. 94, 178–185 (2013)

    Article  CAS  PubMed  Google Scholar 

  15. M.S. Silva, M.C. Brum, E.L. Loreto, R. Weiblen, E.F. Flores, Virus Res. 129, 191–199 (2007)

    Article  CAS  PubMed  Google Scholar 

  16. R.W. Ely, J.M. d’Offay, A.H. Ruefer, C.Y. Cash, J. Vet. Diagn. Invest. 8, 487–492 (1996)

    Article  CAS  PubMed  Google Scholar 

  17. H. Furuoka, N. Izumida, M. Horiuchi, S. Osame, T. Matsui, Acta Neuropathol. 90, 565–571 (1995)

    Article  CAS  PubMed  Google Scholar 

  18. C.D. Penny, F. Howie, P.F. Nettleton, N.D. Sargison, A. Schock, Vet. Rec. 151, 89–91 (2002)

    Article  CAS  PubMed  Google Scholar 

  19. S. Roels, G. Charlier, C. Letellier, G. Meyer, F. Schynts, P. Kerkhofs, E. Thiry, E. Vanopdenbosch, Vet. Rec. 146, 586–588 (2000)

    Article  CAS  PubMed  Google Scholar 

  20. D.R. Rissi, F. Pierezan, M.S. e Silva, E.F. Flores, C.S. de Barros, J. Vet. Diagn. Invest. 20, 346–349 (2008)

    Article  PubMed  Google Scholar 

  21. S.K. Tikoo, D.R. Fitzpatrick, L.A. Babiuk, T.J. Zamb, J. Virol. 64, 5132–5142 (1990)

    CAS  PubMed Central  PubMed  Google Scholar 

  22. E. Gabev, K. Tobler, C. Abril, M. Hilbe, C. Senn, M. Franchini, G. Campadelli-Fiume, C. Fraefel, M. Ackermann, J. Virol. 84, 5583–5593 (2010)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. O.Y. Abdelmagid, H.C. Minocha, J.K. Collins, S.I. Chowdhury, Virology 206, 242–253 (1995)

    Article  CAS  PubMed  Google Scholar 

  24. S.K. Tikoo, T.J. Zamb, L.A. Babiuk, J. Virol. 67, 2103–2109 (1993)

    CAS  PubMed Central  PubMed  Google Scholar 

  25. K.M. Izumi, J.G. Stevens, J. Exp. Med. 172, 487–496 (1990)

    Article  CAS  PubMed  Google Scholar 

  26. G. Campadelli-Fiume, M. Amasio, E. Avitabile, A. Cerretani, C. Forghieri, T. Gianni, L. Menotti, Rev. Med. Virol. 17, 313–326 (2007)

    Article  CAS  PubMed  Google Scholar 

  27. P.G. Spear, Cell Microbiol. 6, 401–410 (2004)

    Article  CAS  PubMed  Google Scholar 

  28. S.A. Connolly, J.J. Whitbeck, A.H. Rux, C. Krummenacher, S. van DrunenLittel, van den Hurk, G.H. Cohen, R.J. Eisenberg, Virology 280, 7–18 (2001)

    Article  CAS  PubMed  Google Scholar 

  29. X. Liang, C. Pyne, Y. Li, L.A. Babiuk, J. Kowalski, Virology 207, 429–441 (1995)

    Article  CAS  PubMed  Google Scholar 

  30. S.K. Tikoo, M. Campos, L.A. Babiuk, Adv. Virus Res. 45, 191–223 (1995)

    Article  CAS  PubMed  Google Scholar 

  31. P.G. Spear, R. Longnecker, J. Virol. 77, 10179–10185 (2003)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  32. C. Ros, S. Belak, J Clin. Microbiol. 37, 1247–1253 (1999)

    CAS  PubMed Central  PubMed  Google Scholar 

  33. S.E. Ashbaugh, K.E. Thompson, E.B. Belknap, P.C. Schultheiss, S. Chowdhury, J.K. Collins, J. Vet. Diagn. Invest. 9, 387–394 (1997)

    Article  CAS  PubMed  Google Scholar 

  34. E.F. Flores, R.O. Donis, Virology 208, 565–575 (1995)

    Article  CAS  PubMed  Google Scholar 

  35. R. Staden, Mol. Biotechnol. 5, 233–241 (1996)

    Article  CAS  PubMed  Google Scholar 

  36. S.F. Altschul, T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller, D.J. Lipman, Nucleic Acids Res. 25, 3389–3402 (1997)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  37. T.A. Hall, Nucleic Acids Symp. Ser. 41, 95–98 (1999)

    CAS  Google Scholar 

  38. J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucleic Acids Res. 25, 4876–4882 (1997)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  39. D. Posada, K.A. Crandall, Bioinformatics 14, 817–818 (1998)

    Article  CAS  PubMed  Google Scholar 

  40. K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731–2739 (2011)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  41. M. Nei, S. Kumar, Molecular evolution and phylogenetics ((Oxford University Press, New York, 2000)

    Google Scholar 

  42. N. Saitou, M. Nei, Mol. Biol. Evol. 4, 406–425 (1987)

    CAS  PubMed  Google Scholar 

  43. J. Felsenstein, Evolution 39, 783–791 (1985)

    Article  Google Scholar 

  44. S. Kumar, K. Tamura, M. Nei, Brief. Bioinform. 5, 150–163 (2004)

    Article  CAS  PubMed  Google Scholar 

  45. P.A. Esteves, O.A. Dellagostin, L.S. Pinto, A.D. Silva, F.R. Spilki, J.R. Ciacci-Zanella, S.O. Hubner, R. Puentes, J. Maisonnave, A.C. Franco, F.A. Rijsewijk, H.B. Batista, T.F. Teixeira, D. Dezen, A.P. Oliveira, C. David, C.W. Arns, P.M. Roehe, Virus Res. 131, 16–22 (2008)

    Article  CAS  PubMed  Google Scholar 

  46. P.D. Giovine, E.C. Settembre, A.K. Bhargava, M.A. Luftig, H. Lou, G.H. Cohen, R.J. Eisenberg, C. Krummenacher, A. Carfi, Plos Pathog. 7, 1–13 (2011)

    Article  Google Scholar 

  47. J.F. Crow, M. Kimura, An introduction to population genetics theory (Harper and Row, New York, 1970)

    Google Scholar 

  48. L.M. Smith, A.R. McWhorter, G.R. Shellam, A.J. Redwood, Virology 435, 258–268 (2013)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). E. F. Flores, R. Weiblen, and F. R. Spilki are CNPq research fellows.

Author information

Authors and Affiliations

  1. Laboratoire des Maladies Infectieuses Virales Vétérinaires (LMIVV), Département de Pathologie et Microbiologie, Faculté de Médicine Vétérinaire, Université de Montréal, 3200 rue Sicotte, Saint Hyacinthe, QC, J2S 7C6, Canada

    Carolina Kist Traesel

  2. United States Department of Agriculture – Agricultural Research Service (USDA-ARS), Southeast Poultry Research Laboratory, 934 College Station Road, Athens, GA, 30605, USA

    Mariana Sá e Silva

  3. Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria (UFSM), Av. Roraima, n.1000, Prédio 20, Sala 4200, Santa Maria, RS, 97105-900, Brazil

    Marcelo Weiss, Rudi Weiblen & Eduardo Furtado Flores

  4. Laboratório de Microbiologia Molecular, Universidade Feevale, RS-239, n.2755, Novo Hamburgo, RS, 93352-000, Brazil

    Fernando Rosado Spilki

Authors
  1. Carolina Kist Traesel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mariana Sá e Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marcelo Weiss
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fernando Rosado Spilki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rudi Weiblen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Eduardo Furtado Flores
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Carolina Kist Traesel.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Traesel, C.K., Sá e Silva, M., Weiss, M. et al. Genetic diversity of 3′ region of glycoprotein D gene of bovine herpesvirus 1 and 5. Virus Genes 48, 438–447 (2014). https://doi.org/10.1007/s11262-014-1040-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-014-1040-5

Keywords

Search

Navigation