Skip to main content
SpringerLink
Log in

Molecular characterization and pathogenicity of tomato yellow leaf curl virus in China

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Several tomato production regions in China were surveyed for tomato yellow leaf curl disease (TYLCD), and 31 tomato leaf samples showing TYLCD-like symptoms were collected. The partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (TYLCV) was detected in Shanghai, Zhejiang, Jiangsu Shandong and Hebei provinces of China. The TYLCV isolates found in China share high sequence identity (>98%) and have more than 97% sequence identity with TYLCV-IL[IL:Reo] (X15656). Phylogenetic relationship analysis reveals that although with little genetic variability, they can form two groups and all the TYLCV isolates in China belong to the group I. An infectious clone of TYLCV-[CN:SH2] (AM282874) was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum, Petunia hybrida, Cucumis sativus, Gossypium hirsutum, S. melongena, and Capsicum annuum. TYLCV-[CN:SH2] can induce severe leaf curling and stunting symptoms in these plants except C. sativus, G. hirsutum, S. melongena and C. annuum. We verified that TYLCV can trans-replicate tomato yellow leaf curl China virus DNA-β in N. benthamiana and S. lycopersicum and induced more severe symptoms with distortion and yellow vein.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. C.M. Fauquet, R.W. Briddon, J.K. Brown, E. Moriones, J. Stanley, M. Zerbini, X. Zhou, Arch. Virol. 153, 783–821 (2008)

    Article  CAS  PubMed  Google Scholar 

  2. C.M. Fauquet, D.M. Bisaro, R.W. Briddon, J.K. Brown, B.D. Harrison, E.P. Rybicki, D.C. Stenger, J. Stanley, Arch. Virol. 148, 405–421 (2003)

    Article  CAS  PubMed  Google Scholar 

  3. A.S. Moffat, Science 286, 1835–1837 (1999)

    Article  CAS  Google Scholar 

  4. B.D. Harrison, D.J. Robinson, Annu. Rev. Phytopathol. 37, 369–398 (1999)

    Article  CAS  PubMed  Google Scholar 

  5. I.B. Dry, J.E. Rigden, L.R. Krake, P.M. Mullineaux, M.A. Rezaian, J. Gen. Virol. 74, 147–151 (1993)

    Article  CAS  PubMed  Google Scholar 

  6. N. Navot, E. Pichersky, M. Zeidan, D. Zamir, H. Czosnek, Virology 185, 151–161 (1991)

    Article  CAS  PubMed  Google Scholar 

  7. Y. Antignus, S. Cohen, Phytopathology 84, 707–712 (1994)

    Article  CAS  Google Scholar 

  8. H. Czosnek, H. Laterrot, Arch. Virol. 142, 1391–1406 (1997)

    Article  CAS  PubMed  Google Scholar 

  9. H. Delatte, D.P. Martin, F. Naze, R. Goldbach, B. Reynaud, M. Peterschmitt, J.M. Lett, J. Gen. Virol. 86, 1533–1542 (2005)

    Article  CAS  PubMed  Google Scholar 

  10. B. Pico, M.J. Diez, F. Nuez, Sci. Hortic. (Amsterdam) 67, 151–196 (1996)

    Article  Google Scholar 

  11. J.E. Polston, P.K. Anderson, Plant. Dis. 81, 1358–1369 (1997)

    Article  PubMed  Google Scholar 

  12. J.B. Wu, F.M. Dai, X.P. Zhou, Plant. Dis. 90, 1359 (2006)

    Article  CAS  PubMed  Google Scholar 

  13. R.B. Mughra, S.S. Liu, X. Zhou, J. Phytopathol. 156, 217–221 (2008)

    Article  Google Scholar 

  14. Y. Xie, X.P. Zhou, Z.H. Li, Z.K. Zhang, G.X. Li, Chin. Sci. Bull. 47, 1273–1276 (2002)

    Article  CAS  Google Scholar 

  15. X.P. Zhou, Y. Xie, Z.K. Zhang, Arch. Virol. 146, 1599–1606 (2001)

    Article  CAS  PubMed  Google Scholar 

  16. J. Rozas, J.C. Sanchez-DelBarrio, X. Messeguer, R. Rozas, Bioinformatics 19, 2496–2497 (2003)

    Article  CAS  PubMed  Google Scholar 

  17. F.A. Vanengelen, J.W. Molthoff, A.J. Conner, J.P. Nap, A. Pereira, W.J. Stiekema, Transgenic. Res. 4, 288–290 (1995)

    Article  CAS  Google Scholar 

  18. X.P. Zhou, Y. Xie, X.R. Tao, Z.K. Zhang, Z.H. Li, C.M. Fauquet, J. Gen. Virol. 84, 237–247 (2003)

    Article  CAS  PubMed  Google Scholar 

  19. Y. Xie, T. Jiang, X.P. Zhou, J. Eur. Plant Pathol. 115, 369–375 (2006)

    Article  Google Scholar 

  20. Z.H. Li, X.P. Zhou, X. Zhang, Y. Xie, Arch. Virol. 149, 1721–1732 (2004)

    CAS  PubMed  Google Scholar 

  21. Q.Y. Yin, H.Y. Yang, Q.H. Gong, H.Y. Wang, Y.L. Liu, Y.G. Hong, P. Tien, Virus Res. 81, 69–76 (2001)

    Article  CAS  PubMed  Google Scholar 

  22. Y. Xie, X.P. Zhou, Arch. Virol. 148, 2047–2054 (2003)

    Article  CAS  PubMed  Google Scholar 

  23. Y.M. Zubiaur, I. Zabalgogeazcoa, C. DeBlas, F. Sanchez, E.L. Peralta, J. Romero, F. Ponz, J. Phytopathol. 144, 277–279 (1996)

    Article  Google Scholar 

  24. J. Navas-Castillo, S. Sanchez-Campos, J.A. Diaz, E. Saez-Alonso, E. Moriones, Plant Dis. 83, 29–32 (1999)

    Article  PubMed  Google Scholar 

  25. S. Duffy, E.C. Holmes, Appl. Environ. Microbiol. 73, 7114–7117 (2007)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. M. Onuki, Nougyou Engei 75, 108–113 (2000)

    Google Scholar 

  27. R.W. Briddon, S.E. Bull, S. Mansoor, I. Amin, P.G. Markham, Mol. Biotechnol. 20, 315–318 (2002)

    Article  CAS  PubMed  Google Scholar 

  28. J. Jose, R. Usha, Virology 305, 310–317 (2003)

    Article  CAS  PubMed  Google Scholar 

  29. K. Saunders, I.D. Bedford, R.W. Briddon, P.G. Markham, S.M. Wong, J. Stanley, Proc. Natl Acad. Sci. USA 97, 6890–6895 (2000)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grants No. 30530520) and the Zhejiang Agricultural Science and Technology Key Research Projects (2007C12054).

Author information

Authors and Affiliations

  1. Key Laboratory of Molecular Biology of Crop Pathogens and Insects of MOA, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, People’s Republic of China

    Hui Zhang, Huanran Gong & Xueping Zhou

  2. Horticultural Research Institute, Shanghai Academy of Agriculture Sciences, 35 Nanhua Rd, Shanghai, 201106, People’s Republic of China

    Hui Zhang

Authors
  1. Hui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Huanran Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xueping Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xueping Zhou.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, H., Gong, H. & Zhou, X. Molecular characterization and pathogenicity of tomato yellow leaf curl virus in China. Virus Genes 39, 249–255 (2009). https://doi.org/10.1007/s11262-009-0384-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-009-0384-8

Keywords

Search

Navigation