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Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane

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Abstract

Sixty-three sugarcane leaf samples were collected from fifty-eight sugarcane varieties, evolved from eleven major sugarcane growing states in India, Australia, South Africa and USA. In RT-PCR, using gene specific primers for sugarcane streak mosaic virus (SCSMV)-CP, 58 of 63 sugarcane samples were found positive to the virus infection and rest of the five samples were negative. Partial CP gene sequences of 42 SCSMV isolates including an isolate from aphid colony (Melanaphis indosacchari) infested on sugarcane variety from this study were characterized after cloning and sequencing for selective isolates represented by at least one isolate from each location. The new sequences identified in the study were named as SCSMV-CB isolates. Fifty two sequences including the 10 database sequences (complete CP cds) deposited earlier from this institute were compared with each other as well as GenBank database sequences of Potyviridae members viz., Rymovirus, Potyvirus, Ipomovirus, Tritimovirus and eight sequences of SCSMV reported from elsewhere. Among the SCSMV-CB isolates sequenced in the study, 85.7–100% (nucleotide) and 89.9–100% (amino acid) sequence identities were observed and with the other data base sequences of SCSMV, the respective identities were 82.2–97.5 and 89.7–98.6%. Grouping of the isolates by the maximum likelihood with molecular clock model, distributed 60 SCSMV sequences including the eight database sequences deposited by other SCSMV working groups from India and USA in 16 different phylogenetic groups. Although the isolates of SCSMV were relatively close to Ipomovirus and Tritimovirus, they were sandwiched between Rymovirus and Ipomovirus. The sequence comparison and phylogenetic studies revealed that the relatedness of SCSMV with the potyviral related genera was comparatively low to consider it as a member of earlier described potyviral genera, hence the genus “Susmovirus” (sugarcane streak mosaic virus) has been proposed, with SCSMV as the sole species to be included. The 52 SCSMV-CB isolates from this institute were distributed in 14 phylogenetic groups and the grouping pattern revealed that the virus isolates could not be grouped based on geographical origin of the host varieties or longevity of the host variety.

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Acknowledgements

The authors wish to express sincere thanks to Dr. N. Vijayan Nair, Director of the Institute for the encouragement and facilities. Financial support received from ICAR, New Delhi as part of Network Project on Developing Diagnostics of Emerging Plant Viruses is gratefully acknowledged.

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  1. Plant Pathology Section, Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, 641007, India

    R. Viswanathan, M. Balamuralikrishnan & R. Karuppaiah

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  1. R. Viswanathan
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  2. M. Balamuralikrishnan
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  3. R. Karuppaiah
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Correspondence to R. Viswanathan.

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Viswanathan, R., Balamuralikrishnan, M. & Karuppaiah, R. Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane. Virus Genes 36, 553–564 (2008). https://doi.org/10.1007/s11262-008-0228-y

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