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Genomic Features of Attenuated Junín Virus Vaccine Strain Candidate

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Abstract

Junin virus strain Candid #1 was developed as a live attenuated vaccine for Argentine haemorrhagic fever. In this paper, we report the nucleotide sequences of L RNA of Candid #1 and examine the relationship to its more virulent ancestors Junin virus XJ#44 and XJ 13 (prototype) and other closely and distantly related arenaviruses. Comparisons of the nucleotide and amino acid sequences of L and Z genes of Candid #1 and its progenitor strains revealed twelve point mutations in the L polypeptide that are unique to the vaccine strain. These changes could be provisionally associated with the attenuated phenotype. In contrast, Z ORF was completely conserved among all strains.

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References

  1. M.H.V. van Regenmortel, C.M. Fauquet, D.H.L. Bishop, E.B. Carstens, M.K. Estes, S.M. Lemon, J. Maniliff, M.A. Mayo, D.J. McGeoch, C.R. Pringle, R.B. Wickner (eds.), Seventh ICTV Report (Academic Press, San Diego, 2000)

  2. C.A. Tidona, G. Darai (eds.), The Springer Index of Viruses (Springer, Heidelberg, Berlin, 2001)

  3. J.B. García, S.P. Morzunov, S. Levis, J. Rowe, G. Calderón, D. Enría, M. Sabattini, M.J. Buchmeier, M.D. Bowen, S.C. St. Jeor, Virology 272, 127–136 (2000)

    Article  PubMed  Google Scholar 

  4. J.C.S. Clegg, M.D. Bowen, M.J. Buchmeier, J-P. Gonzalez, I.S. Lukashevich, C.J. Peters, R. RicoHesse, V. Romanowski, in Arenaviridae in Seventh Report of the International Committee on Taxonomy of Viruses, ed. by M.H.V. van Regenmortel, C.M. Fauquet, D.H.L. Bishop, E.B. Carstens, M.K. Estes, S.M. Lemon, J. Maniliff, M.A. Mayo, D.J. McGeoch, C.R. Pringle, R.B. Wickner, (Academic Press, San Diego, 2000), pp. 633–640

  5. J.I. Maiztegui, J.G. Barrera Oro, M.R. Feuillade, C.J. Peters, D. Vallejos, K.T. McKee, Inoculation of human volunteers with Candid #1, a live attenuated Junín virus vaccine candidate, VIIIth International Congress of Virology (Abstracts Book, Berlin, Germany, 1990) p. 111

  6. A.S. Parodi, D.J. Greenway, H.R. Rugiero, E. Rivero, M.J. Frigerio, N.E. Mettler, F. Garzon, M. Boxaca, L.B. Guerrero, N.R. Nota, Sobre la etiología del brote epidémico en Junín Día Médico (Buenos Aires) 30, 2300–2302 (1958)

    CAS  Google Scholar 

  7. P.D. Ghiringhelli, C.G. Albariño, M. Piboul, V. Romanowski, Am. J. Trop. Med. Hyg. 78, 1605–1610, (1996)

    Google Scholar 

  8. P.D. Ghiringhelli, R.V. Rivera-Pomar, M.E. Lozano, O. Grau, V. Romanowski, J. Gen. Virol. 72, 2129–2141, (1991)

    CAS  PubMed  Google Scholar 

  9. J.A. Cuff, G.J. Barton, Application of multiple sequence alignment profiles to improve protein secondary structure prediction Proteins 40, 502–511, (2000)

    CAS  Google Scholar 

  10. S. Vieth, A.E. Torda, M. Asper, H. Schmitz, S. Günter, Virology 318, 153–168 (2004)

    Article  CAS  PubMed  Google Scholar 

  11. J.M. McAuliffe, S.R. Surman, J.T. Newman, J.M. Riggs, P.L. Collins, B.R. Murphy, M.H. Skiadopoulos, J. Virol. 78(4), 2029–2036 (2004)

    Article  CAS  PubMed  Google Scholar 

  12. E. Fodor, L.J. Mingay, M. Crow, T. Deng, G.G. Brownlee, J. Virol. 77(8), 5017–5020 (2003)

    Article  CAS  PubMed  Google Scholar 

  13. C.G. Albariño, P.D. Ghiringhelli, D.M. Posik, M.E. Lozano, A.M. Ambrosio, A. Sanchez, V. Romanowski, J. Gen. Virol. 78, 1605–1610 (1997)

    PubMed  Google Scholar 

  14. I vanKnippenberg, R. Goldbach, R. Kormelink, Virus Res. 110, 125–131 (2005)

    PubMed  Google Scholar 

  15. M.A. Tortorici, C.G. Albariño, D.M. Posik, P.D. Ghiringhelli, M.E. Lozano, R.V Rivera Pomar, V. Romanowski, Virus Res. 73, 41–55 (2001)

    Article  CAS  PubMed  Google Scholar 

  16. D.D. Pinschewer, M. Perez, J.C. de la Torre, J. Virol. 79(7), 4519–4526 (2005)

    Article  CAS  PubMed  Google Scholar 

  17. D. Garcin, D. Kolakofsky, Virology 66, 1370–1376 (1992)

    CAS  Google Scholar 

  18. B.J. Meyer, J.C. de la Torre, P.J. Southern, Curr. Top. Microbiol. Immunol. 262, 139–157 (2002)

    CAS  PubMed  Google Scholar 

  19. R.N. Charrell, J.J. Lemasson, M. Garbutt, R. Khelifa, P. De Micco, H. Feldmann, X. de Lamballerie, Virology 317, 191–196 (2003)

    Google Scholar 

  20. M.D. Bowen, C.J. Peters, S.T. Nichol, Mol. Phylogenet. Evol. 8, 301–316 (1997)

    CAS  PubMed  Google Scholar 

  21. J.N. Barr, S.P. Whelan, G.W. Wertz, Biochim. Biophys. Acta 1577(2), 337–353 (2002)

    CAS  PubMed  Google Scholar 

  22. A.C. Lowen, A. Boyd, J.K. Fazakerley, R.M. Elliott, J. Virol. 79, 6940–6946 (2005)

    CAS  PubMed  Google Scholar 

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Acknowledgements

We are very grateful to Dr. J. G Barrera Oro from USAMRJID (Frederick, Maryland, USA) who provided us with an XJ#44 seed and to Dr. María del Carmen Saavedra who prepare Candid #1 virions. This work was supported by PICT 6654 from ANPCYT and PIP 1076 from CONICET (Argentina). S. G. had a research fellowship from ANPCYT and now holds a research fellowship from CONICET, J. I. holds a research fellowship from CONICET and M. E. L., P. D. G. and V. R. are members of the research career of CONICET.

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Authors and Affiliations

  1. LIGBCM, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Rogue Saenz Peña 180, B1876BXD, Bernal, Buenos Aires, Argentina

    Sandra Elizabeth Goñi, Javier Alonso Iserte, Victor Romanowski, Pablo Daniel Ghiringhelli & Mario Enrique Lozano

  2. Instituto Nacional de Enfermedades Virales Humanas “Dr. Julio I. Maiztegui”,, Monteagudo 2510, 2700, Pergamino, Argentina

    Ana Maria Ambrosio

  3. lnstituto de Bioquimica y Biologia Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 49 y 115, 1900, La Plata, Argentina

    Victor Romanowski

Authors
  1. Sandra Elizabeth Goñi
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  2. Javier Alonso Iserte
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  3. Ana Maria Ambrosio
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  4. Victor Romanowski
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  5. Pablo Daniel Ghiringhelli
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  6. Mario Enrique Lozano
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Corresponding author

Correspondence to Mario Enrique Lozano.

Additional information

The nucleotide sequences data of the of the L RNA of the Junin virus strains reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers: AY819707.

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Goñi, S.E., Iserte, J.A., Ambrosio, A.M. et al. Genomic Features of Attenuated Junín Virus Vaccine Strain Candidate. Virus Genes 32, 37–41 (2006). https://doi.org/10.1007/s11262-005-5843-2

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  • DOI: https://doi.org/10.1007/s11262-005-5843-2

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