Abstract
Shiga toxin-producing Escherichia coli (STEC) are recognized as being responsible for many cases of foodborne diseases worldwide. Cattle are the main reservoir of STEC, shedding the microorganisms in their feces. The serogroup STEC O91 has been associated with hemorrhagic colitis and hemolytic uremic syndrome. Locus of Adhesion and Autoaggregation (LAA) and its hes gene are related to the pathogenicity of STEC and the ability to form biofilms. Considering the frequent isolation of STEC O91, the biofilm-forming ability, and the possible role of hes in the pathogenicity of STEC, we propose to evaluate the ability of STEC to form biofilms and to evaluate the expression of hes before and after of biofilm formation. All strains were classified as strong biofilm-forming. The hes expression showed variability between strains before and after biofilm formation, and this may be due to other genes carried by each strain. This study is the first to report the relationship between biofilm formation, and hes expression and proposes that the analysis and diagnosis of LAA, especially hes as STEC O91 virulence factors, could elucidate these unknown mechanisms. Considering that there is no specific treatment for HUS, only supportive care, it is necessary to know the survival and virulence mechanisms of STEC O91.
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Acknowledgements
The authors thank María Rosa Ortíz for her technical assistance.
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This work was supported by PICT 2015–2666, CIC, and SECAT from Argentina.
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Conceptualization, R. Colello., M.V. Vélez, and N.L. Padola.; Funding acquisition, N.L. Padola.; Investigation, R. Colello. and M.V. Vélez.; Methodology, M.V. Vélez and L. E. Paz.; Supervision, N.L. Padola. and A.I. Etcheverría.; Validation, M.V.Vélez and M.V. Nieto Farias.; Visualization, R. Colello., and N.L. Padola.; Writing-original draft, M.V. Vélez.; Writing-review and editing, R. Colello., M.V. Vélez., M.V. Nieto Farias., R. Vidal., A.I. Etcheverria. and N.L. Padola.
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Vélez, M.V., Colello, R., Nieto, M.V. et al. Transcription levels of hes and their involvement in the biofilm formation of Shiga toxin-producing Escherichia coli O91. Vet Res Commun 48, 1821–1830 (2024). https://doi.org/10.1007/s11259-024-10308-0
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DOI: https://doi.org/10.1007/s11259-024-10308-0